A5018111436- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Neural dynamics and brain function
- Ion channel regulation and function
- Cellular transport and secretion
- Advanced Electron Microscopy Techniques and Applications
- Photoreceptor and optogenetics research
- Cell Image Analysis Techniques
- Pain Mechanisms and Treatments
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Neurogenesis and neuroplasticity mechanisms
- Neurobiology and Insect Physiology Research
- Neuroscience and Neural Engineering
- Retinal Development and Disorders
- Memory and Neural Mechanisms
- Advanced Chemical Sensor Technologies
- Biochemical Analysis and Sensing Techniques
- Neuroinflammation and Neurodegeneration Mechanisms
- Single-cell and spatial transcriptomics
- Nicotinic Acetylcholine Receptors Study
- Advanced Biosensing Techniques and Applications
- Glioma Diagnosis and Treatment
- Anatomy and Medical Technology
Heidelberg University
2016-2025
Heidelberg University
2006-2025
German Center for Lung Research
2020-2024
University Hospital Heidelberg
2006-2022
Institute of Pathology Celle
2018-2021
Karlsruhe Institute of Technology
2021
Max Planck Institute for Medical Research
2004-2019
Max Planck Society
1996-2011
Cold Spring Harbor Laboratory
2011
Duke University
2000-2010
AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid) receptor channels mediate the fast component of excitatory postsynaptic currents in central nervous system. Site-selective nuclear RNA editing controls calcium permeability these channels, and at a second site is shown here to affect kinetic aspects rat brain. In three four subunits (GluR-B, -C, -D), intronic elements determine codon switch (AGA, arginine, GGA, glycine) primary transcripts position termed R/G site, which...
This is the first article in a series of 3 studies that investigate anatomical determinants thalamocortical (TC) input to excitatory neurons cortical column rat primary somatosensory cortex (S1). S1 receives 2 major types TC inputs, lemiscal and paralemniscal. Lemiscal axons arise from ventral posteromedial nucleus (VPM) thalamus, whereas paralemniscal fibers originate (POm). While these projections are largely complementary L4, overlap other layers still matter debate. VPM POm were...
Abstract Nerve injury leads to chronic pain and exaggerated sensitivity gentle touch (allodynia) as well a loss of sensation in the areas which injured non-injured nerves come together 1–3 . The mechanisms that disambiguate these mixed paradoxical symptoms are unknown. Here we longitudinally non-invasively imaged genetically labelled populations fibres sense noxious stimuli (nociceptors) (low-threshold afferents) peripherally skin for longer than 10 months after nerve injury, while...
Humans and old world primates have trichromatic color vision based on three spectral types of cone [long-wavelength (L-), middle-wavelength (M-), short-wavelength (S-) cones]. All other placental mammals are dichromats, their depends the comparison L- S-cone signals; however, cone-selective retinal circuitry is still unknown. Here, we identified S-cone-selective (blue cone) bipolar cells mouse retina. They were labeled in a transgenic expressing Clomeleon, chloride-sensitive fluorescent...
In NMDA receptor channels, subtype-specific differences of Mg2+ block are determined by the NR2 subunits. Channels assembled from NR1-NR2A or NR1-NR2B subunits blocked more strongly than channels formed NR1-NR2C NR1-NR2D subunits, predominantly reflecting a difference in voltage dependence. A determinant common to is located M2 domain (N-site Q/R/N-site). However, subunit-specific suggested that additional structural elements exist. Chimeric were constructed replacing segments least...
The structure of the NMDA receptor channel M2 segment was investigated by probing extracellular and cytoplasmic faces cysteine-substituted NR1-NR2C channels with charged sulfhydryl-specific reagents. pattern accessible positions suggests that forms a channel-lining loop originating ending on side channel, ascending limb in an alpha-helical descending extended structure. A functionally critical asparagine (N-site) is positioned at tip loop, cluster hydrophilic residues limb, adjacent to tip,...
Seizures induce excitatory shifts in the reversal potential for GABA(A)-receptor-mediated responses, which may contribute to intractability of electro-encephalographic seizures and preclude efficacy widely used GABAergic anticonvulsants such as phenobarbital. We now report that, intact hippocampi prepared from neonatal rats transgenic mice expressing Clomeleon, recurrent progressively increase intracellular chloride concentration ([Cl(-)](i)) assayed by Clomeleon imaging invert net effect...
High resolution, three-dimensional (3D) representations of cellular ultrastructure are essential for structure function studies in all areas cell biology. While limited subcellular volumes have been routinely examined using serial section transmission electron microscopy (ssTEM), complete ultrastructural reconstructions large volumes, entire cells or even tissue difficult to achieve ssTEM. Here, we introduce a novel approach combining sectioning with scanning (SEM) conductive silicon wafer...
Chemical synapses contain substantial numbers of neurotransmitter-filled synaptic vesicles, ranging from approximately 100 to many thousands. The vesicles fuse with the plasma membrane release neurotransmitter and are subsequently reformed recycled. Stimulation in vitro generally causes majority neurotransmitter, leading assumption that numerous sustain transmission during high activity. We tested this by an approach we termed cellular ethology, monitoring vesicle function behaving animals...
Abstract Understanding the nano-architecture of protein machines in diverse subcellular compartments remains a challenge despite rapid progress super-resolution microscopy. While single-molecule localization microscopy techniques allow visualization and identification cellular structures with near-molecular resolution, multiplex-labeling tens target proteins within same sample has not yet been achieved routinely. However, single multiplexing is essential to detect patterns that threaten get...