A5027089376- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
- Plant and Biological Electrophysiology Studies
- Cardiac electrophysiology and arrhythmias
- Electrochemical Analysis and Applications
- Erythrocyte Function and Pathophysiology
- Retinal Development and Disorders
- Neurobiology and Insect Physiology Research
- Force Microscopy Techniques and Applications
- Plant Molecular Biology Research
- Mast cells and histamine
- Pancreatic function and diabetes
- Nicotinic Acetylcholine Receptors Study
- Photosynthetic Processes and Mechanisms
- Botulinum Toxin and Related Neurological Disorders
- Microfluidic and Bio-sensing Technologies
- Ion Channels and Receptors
- Protein Kinase Regulation and GTPase Signaling
Macau University of Science and Technology
2020-2024
University of Göttingen
1982-2024
Max Planck Institute for Multidisciplinary Sciences
2022-2024
Multiscale Bioimaging
2021-2024
Max Planck Institute for Biophysical Chemistry
2013-2023
Jawaharlal Nehru Centre for Advanced Scientific Research
2023
National Institute for Materials Science
2023
Shanghai University
2023
Keio University
2023
Utrecht University
2023
The capacitance of the surface membrane small adrenal chromaffin cells was measured with patch-clamp pipettes. Continuous and discrete changes were observed. They interpreted as area connected to exocytotic or endocytotic processes. Most measurements performed in "whole-cell" recording configuration [Hamill, O. P., Marty, A., Neher, E., Sakmann, B. & Sigworth, F. J. (1981) Pflügers Arch. 391, 85-100], which allows intracellular Ca2+ concentration be controlled. With an internal solution...
1. Inward currents in chromaffin cells were studied with the patch‐clamp technique (Hamill, Marty, Neher, Sakmann & Sigworth, 1981). The intracellular solution contained 120 mM‐Cs + and 20 mM‐tetraethylammonium (TEA ). Na after blockade of Ca 2+ channels 1 mM‐Co applied externally. recorded eliminating tetrodotoxin (TTX). current recordings obtained cell‐attached, outside‐out whole‐cell recording configurations (Hamill et al. 2. Single channel measurements gave an elementary amplitude pA...
1. Single channel currents through acetylcholine receptor channels (ACh channels) were recorded at chronically denervated frog muscle extrajunctional membranes in the absence and presence of lidocaine derivatives QX‐222 QX‐314. 2. The current wave forms due to opening closing single ACh (activated by suberyldicholine) normally are square pulses. These pulses appear be chopped into bursts much shorter pulses, when drug is present addition agonist. 3. mean duration comparable or longer than...
1. Bovine chromaffin cells were enzymatically isolated and kept in short term tissue culture. Their electrical properties studied using recent advances of the patch‐clamp technique (Hamill, Marty, Neher, Sakmann & Sigworth, 1981). 2. When a patch pipette was sealed tightly to cell ('cell‐attached configuration') current wave forms due intracellular action potentials could be observed. The frequency altered by changing potential. acetylcholine present solution, acetylcholine‐induced...
1. Digital imaging and photometry were used in conjunction with the fluorescent Ca2+ indicator, Fura‐2, to examine intracellular signals produced by depolarization of single adrenal chromaffin cells. 2. Depolarization a patch pipette radial gradients within cell, concentration highest vicinity plasma membrane. These dissipated few hundred milliseconds when voltage‐gated channels closed. 3. Dialysis Fura‐2 into cell caused concentration‐dependent changes depolarization‐induced signal,...