Abstract In humans, gamma-band oscillations in the primary somatosensory cortex (S1) correlate with subjective pain perception. However, functional contributions to and nature of underlying circuits are unclear. Here we report that gamma oscillations, but not other rhythms, specifically strengthened independently any motor component S1 mice during nociception. Moreover, inflammatory show elevated resting alpha activity increased power response sub-threshold stimuli, association behavioral...

The Animal Study Registry (ASR; www.animalstudyregistry.org) was launched in January 2019 for preregistration of animal studies order to increase transparency and reproducibility bioscience research promote welfare. registry is free charge designed exploratory confirmatory within applied science as well basic preclinical research. registration form helps scientists plan their study thoroughly by asking detailed questions concerning design, methods, statistics. With registration, the...

In addition to analgesia, opioids may also produce paradoxical pain amplification [opioid-induced hyperalgesia (OIH)] either on abrupt withdrawal or during continuous long-term application. Here, we assessed antinociceptive and pronociceptive effects of three clinically used at C-fiber synapses in the rat spinal dorsal horn vivo. During 60 min intravenous infusions remifentanil (450 μg·kg⁻¹·h⁻¹), fentanyl (48 morphine (14 mg·kg⁻¹·h⁻¹), C-fiber-evoked field potentials were depressed...

This report describes a step-by-step guide to the technique of acute intrathecal needle injections in noninvasive manner, i.e. independent catheter implantation. The technical limitation this surgical lies finesse hands. injection is rapid, especially for trained experimenter, and since tissue disruption with minimal, repeated are possible; moreover immune reaction foreign tools (e.g. catheter) does not occur, thereby giving better more specific read out spinal cord modulation. Since...

Translational biomedical research relies on animal experiments and provides the underlying proof of practice for clinical trials, which places an increased duty care translational researchers to derive maximum possible output from every experiment performed. The implementation open science practices has potential initiate a change in culture that could improve transparency quality general, as well increasing audience scientific reach published research. However, become buzzword community can...

Buprenorphine is a commonly used opioid to treat moderate severe pain in mice. Although strain differences regarding basal sensitivity and the analgesic effect of other opioids have been described for mice, data buprenorphine incomplete. Hence, we investigated (0.42, 4.0 mg·kg-1) male C57BL/6J, Balb/cJ 129S1/SvImJ mice using incremental hot plate. Additionally, verified single nucleotide polymorphisms Cytochrome P450 3a (Cyp3a) genes, which encode enzymes that are relevant metabolism,...

Abstract Preregistration of studies is a recognized tool in clinical research to improve the quality and reporting all gained results. In preclinical research, preregistration could boost translation published results into breakthroughs. When rely on animal testing or form basis trials, maximizing validity reliability outcomes becomes addition an ethical obligation. Nevertheless, implementation still slow. However, institutions, funders, publishers start valuing preregistration, thereby...

Opioids are the most powerful analgesics available to date. However, they may also induce adverse effects including paradoxical opioid-induced hyperalgesia. A mechanism that might underlie hyperalgesia is amplification of synaptic strength at spinal C-fibre synapses after withdrawal from systemic opioids such as remifentanil ("opioid-withdrawal long-term potentiation [LTP]"). Here, we show both induction well maintenance opioid-withdrawal LTP were abolished by pharmacological blockade glial...

Science & Society23 December 2019Open Access Rethinking the incentive system in science: animal study registries Preregistering experiments using animals could greatly improve transparency and reliability of biomedical studies welfare Céline Heinl German Federal Institute for Risk Assessment, Centre Protection Laboratory Animals (Bf3R), Berlin, Germany Search more papers by this author Justyna Chmielewska Anastasia Olevska Barbara Grune Gilbert Schönfelder Corresponding Author [email...

This report describes a step-by-step guide to the technique of acute intrathecal needle injections in noninvasive manner, i.e. independent catheter implantation. The technical limitation this surgical lies finesse hands. injection is rapid, especially for trained experimenter, and since tissue disruption with minimal, repeated are possible; moreover immune reaction foreign tools (e.g. catheter) does not occur, thereby giving better more specific read out spinal cord modulation. Since...

The scientific community widely discusses preregistration. main idea of preregistration is being able to untangle the a priori hypothesis from outcome-driven and exploratory analyses once data generated. Researchers can ensure neutrality towards their an objective evaluation study outcomes by cultivating record study, starting with plan. This especially valuable during peer review process assisting publishers in retracing generation analysis. have couple options for sharing methods, plans,...

<ns4:p>The translation of animal-based biomedical research into clinical is often inadequate. Maximizing should be central to animal on human diseases, guiding researchers in study design and model selection. However, practical considerations drive the choice model, which may not always reflect key patient characteristics, such as sex age, impacting disease's course. Despite diseases affecting both sexes, frequently use male mice. To address this imbalance, journals funding agencies have...

Fast and effective anesthesia is the key for refining many invasive procedures in fish gaining reliable data. For as all vertebrates, it also required by European law to reduce pain, suffering, distress unavoidable minimum husbandry experiments. The most often used substance induce zebrafish tricaine (MS-222). When properly prepared dosed, causes rapid loss of mobility, balance reaction touch. These signs are interpreted a stage deep although its effects on central nervous system have not...

The translation of animal-based biomedical research into clinical is often inadequate. Maximizing should be central to animal on human diseases, guiding researchers in study design and model selection. However, practical considerations drive the choice model, which may not always reflect key patient characteristics, such as sex age, impacting disease's course. Despite diseases affecting both sexes, frequently use male mice. To address this imbalance, journals funding agencies have begun...