A5035220432- Alzheimer's disease research and treatments
- Neuroscience and Neuropharmacology Research
- Immunotherapy and Immune Responses
- Tryptophan and brain disorders
- Immune Cell Function and Interaction
- Nuclear Receptors and Signaling
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Neuroinflammation and Neurodegeneration Mechanisms
- Antimicrobial Peptides and Activities
- Olfactory and Sensory Function Studies
- Receptor Mechanisms and Signaling
- Retinal Development and Disorders
- Peroxisome Proliferator-Activated Receptors
- RNA regulation and disease
- Diet and metabolism studies
- Glycosylation and Glycoproteins Research
- Endoplasmic Reticulum Stress and Disease
- Nicotinic Acetylcholine Receptors Study
- Cell Adhesion Molecules Research
- Genetic Syndromes and Imprinting
- Memory and Neural Mechanisms
- Cholinesterase and Neurodegenerative Diseases
- 14-3-3 protein interactions
- Retinal Diseases and Treatments
Navarre Institute of Health Research
2016-2025
Universidad de Navarra
2014-2025
Clinica Universidad de Navarra
2021
Instituto de Investigación Sanitaria del Principado de Asturias
2021
University of Tsukuba
2021
Universitat de Barcelona
2016-2021
Weatherford College
2021
Biomedical Research Networking Center on Neurodegenerative Diseases
2009-2016
Bellvitge University Hospital
2016
Institut d'Investigació Biomédica de Bellvitge
2016
The mitochondrial voltage-dependent anion channel 1 (VDAC1) is involved in the release of apoptotic proteins with possible relevance Alzheimer's disease (AD) neuropathology. Through proteomic analysis followed by Western blotting and immunohistochemical techniques, we have found that VDAC1 overexpressed hippocampus from amyloidogenic AD transgenic mice models. was also postmortem brain tissue patients at an advanced stage disease. Interestingly, amyloid-β (Aβ) soluble oligomers were able to...
Synapse loss occurs early in Alzheimer's disease (AD) and is considered thebest pathological correlate of cognitive decline. Ephrins Eph receptorsare involved regulation excitatory neurotransmission play a rolein cytoskeleton remodeling
Patients with Alzheimer's disease (AD) display altered functioning of cortical networks, including patterns synchronous activity and a serious deficit in cholinergic septohippocampal (SH) innervation. However, the mechanisms underlying these alterations implication GABAergic SH component AD are largely unknown. In addition, pathway (SHP) is believed to regulate hippocampal by controlling interneurons. Here we show, using well-characterized tracing experiments, that innervation SHP decreases...
The etiology of the more common (sporadic) forms Alzheimer's disease (AD) remains unknown, although age is most important risk factor. Nevertheless, interactions between environmental factors and genetic background may also influence
GPR40, the free fatty acid receptor 1, is expressed strongly in primate pancreas and brain. While role of pancreatic GPR40 glucose homeostasis has been extensively studied, absence this G-protein-coupled from brain rodents hampered studies into its central nervous system. However, we found intense mRNA expression by situ hybridization mouse hippocampal motor cortex neurons. Furthermore, a neuroblastoma cell was activated docosahexaenoic selective agonists, yet not palmitic acid....
Pigment epithelium-derived factor (PEDF) was identified from retinal pigment epithelial cells and has been shown to display neurotrophic effects. In addition it found induce a potent inhibition of angiogenesis. this study we have explored whether overexpression PEDF by gene transfer approach can block tumor angiogenesis reduce growth. We that infected with an adenovirus encoding under the control CMV promoter (AdPEDF) secreted protein into medium exhibited strong inhibitory effects on...
Pigment epithelium-derived factor (PEDF) is a multifunctional serpin with antitumorigenic, antimetastatic, and differentiating activities. PEDF found within tissues rich in the glycosaminoglycan hyaluronan (HA), its amino acid sequence contains putative HA-binding motifs. We show that coprecipitation glycosaminoglycans media conditioned by human retinoblastoma Y-79 cells decreased after pretreatments hyaluronidase, implying an association between HA PEDF. Direct binding of recombinant to...
Understanding the mechanisms involved in cognitive resilience Alzheimer’s disease (AD) represents a promising strategy to identify novel treatments for dementia AD. Previous findings from our group revealed that study of aged-Tg2576 resilient individuals is suitable tool this purpose. In present study, we performed transcriptomic analysis using prefrontal cortex demented and Tg2576 transgenic AD mice. We have been able hypothesize pathways inflammation, amyloid degradation, memory function,...
The Tg2576 mouse, which carries the Swedish mutant form of human β-amyloid precursor protein (hAPPswe), develops Alzheimer's Disease (AD)-like phenotype (synaptic pathology, cognitive impairment and β amyloid -Aβ- plaques.) in absence significant neuronal loss. We have analyzed hippocampal proteome Tg2576, focusing on changes at 7 months age, when Aβ levels begin to increase but symptoms are still not evident, 16 months, most AD-like features manifested. Proteins differentially expressed...
Alzheimer's disease (AD) might be conceptualized as a metabolic with progressive impairment of the brain's capacity to utilize glucose. One last glucose transporters discovered is GLUT12. The aim present work was investigate
PEDF, a member of the serpin superfamily proteins related through their highly conserved folded conformation, has neurotrophic properties, including promotion neurite-outgrowth and neuronal survival. Previously, we have purified characterized PEDF protein from extracellular matrixes bovine eyes. Here, show cDNA sequence expression analysis PEDF. Northern RNA retinal pigment epithelium (RPE) neural retina using human fragment reveals gene only for RPE. Sequence clone isolated RPE predicts...
Removal of 3'-azido-3'deoxythymidine (AZT) 3'-azido-3'-deoxythymidine 5'-monophosphate (AZTMP) from the terminated primer mediated by human HIV-1 reverse transcriptase (RT) has been proposed as a relevant mechanism for resistance HIV to AZT. Here we compared wild type and AZT-resistant (D67N/K70R/T215Y/K219Q) RTs their ability unblock AZTMP-terminated phosphorolysis in presence physiological concentrations pyrophosphate or ATP. The enzyme, it previously described, showed an increased...
Synucleinopathies are a group of neurodegenerative diseases without effective treatment characterized by the abnormal aggregation alpha-synuclein (aSyn) protein. Changes in levels or amino acid sequence aSyn (by duplication/triplication gene point mutations encoding region) cause familial cases synucleinopathies. However, specific molecular mechanisms aSyn-dependent toxicity remain unclear. Increased protein pathological may favor protein-protein interactions (PPIs) that could either promote...
Abstract The risk of suffering from Alzheimer’s disease (AD) is higher in individuals AD-affected mothers. purpose this investigation was to study whether maternal transmission might produce AD-related alterations progenies mice that do not have any genotypic alteration. We used cognitively-intact mothers harbouring heterozygosity the transgene for overexpressing Swedish double mutant version human amyloid precursor protein (hAβPPswe). phenotype offspring with or without resulting crossing...
The olfactory bulb (OB) is the first processing station in pathway. Despite smell impairment considered an early event Alzheimer´s disease (AD), little known about initial molecular disturbances that accompany AD development at level. We have interrogated time-dependent OB landscape Tg2576 mice prior to appearance of neuropathological amyloid plaques (2-, and 6-month-old), using combinatorial omics analysis. metabolic modulation induced by human mutated precursor protein (APP) overproduction...
Olfaction is often deregulated in Alzheimer's disease (AD) patients, and also impaired transgenic Tg2576 AD mice, which overexpress the Swedish mutated form of human amyloid precursor protein (APP). However, little known about molecular mechanisms that accompany neurodegeneration olfactory structures aged mice. For that, we have applied proteome- transcriptome-wide approaches to probe disturbances bulb (OB) dissected from mice (18 months age) as compared those age matched wild-type (WT)...
Synthetic peptides with sequences present in extracellular matrix proteins are capable of causing the expression inducible form nitric oxide synthase (iNOS), detected by immunocytochemistry, and release NO human lymphomononuclear cells incubated their presence. Active 15‐mers containing a characteristic 2‐6‐11 motif which amino acid residue at position 2 is Leu, Ile, Val, Gly, Ala or Lys; 6 always Pro; 11 Glu Asp. The induction iNOS monocytes macrophages could be involved cytotoxicity...
Understanding how neural cells handle proteostasis stress in the endoplasmic reticulum (ER) is important to decipher mechanisms that underlie cell death associated with neurodegenerative diseases and design appropriate therapeutic tools. Here we have compared sensitivity of a human neuroblastoma line (SH-SY5H) ER caused by an inhibitor protein glycosylation observed embryonic kidney (HEK-293T) cells. In response stress, SH-SY5H increase expression mRNA encoding downstream effectors sensors...