A5047620994- Virus-based gene therapy research
- Herpesvirus Infections and Treatments
- Nerve injury and regeneration
- Alzheimer's disease research and treatments
- Genetic Neurodegenerative Diseases
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Viral Infectious Diseases and Gene Expression in Insects
- Cellular transport and secretion
- RNA Interference and Gene Delivery
- Biotin and Related Studies
- Neuroscience and Neuropharmacology Research
- Viral Infections and Immunology Research
- Toxin Mechanisms and Immunotoxins
- Mitochondrial Function and Pathology
- Protein Structure and Dynamics
- RNA regulation and disease
- Genetics and Neurodevelopmental Disorders
- Chemokine receptors and signaling
- Hereditary Neurological Disorders
- Wnt/β-catenin signaling in development and cancer
- Microtubule and mitosis dynamics
- Viral gastroenteritis research and epidemiology
- Systemic Lupus Erythematosus Research
- Animal Virus Infections Studies
Singapore General Hospital
2021-2023
Universidad Autónoma de Madrid
2011-2022
Centro de Biología Molecular Severo Ochoa
2006-2022
National University Hospital
2020
National University of Singapore
2010-2020
Brown University
2020
KK Women's and Children's Hospital
2019
Centro de Investigación Biomédica en Red
2008-2012
Centre for Biomedical Network Research on Rare Diseases
2008-2012
California State University, Dominguez Hills
2011
The site and regulation of neurotrophic factor release from neurons is poorly understood. We used a combination model cell lines primary culture systems to study the polarity BDNF sorting its hippocampal neurons. Transfection expression human cDNA in mouse pituitary line, AtT20, resulted colocalization with secretory granule marker, chromogranin A. Furthermore, stimulation these cells 56 mM KCl or 5 8-bromo-cAMP increased approximately 10-to 15-fold within 30 min. To cultures neurons, were...
Herpes simplex virus type 1 (HSV-1) plasmid vectors have promise for genetic intervention in the brain, but several problems caused by helper compromised their utility. To develop a virus-free packaging system these vectors, DNA cleavage/packaging signals were deleted from set of cosmids that represents HSV-1 genome. Following cotransfection into cells, this modified cosmid supported replication and vector DNA. However, absence signals, genome was not packaged, consequently stocks free...
Today´s Net Generation university students multitask more than any prior generation, primarily using electronic communication tools (Carrier et al., 2009). In addition, studies report that many text during class (Tindell & Bohlander, 2011). This research examines the impact of receiving and sending messages a classroom lecture. Recent laboratory (Ophir, 2009) reported multitasking impaired performance, particularly among heavy multitaskers. Further, experimental has shown "technologically...
Abstract We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other in three dimensions. Transplantation is being trialled repair the paralysed spinal cord, promising but variable results and thus therapy needs improving. To date, studies OEC behaviour multicellular environment have been hampered by lack suitable cell culture models. Here, we exploit floating liquid marble, droplet coated...
Tau protein hyperphosphorylation and aggregation into neurofibrillary tangles are characteristic features of several neurodegenerative disorders referred to as tauopathies. Among them, frontotemporal dementia Parkinsonism linked chromosome 17
A key feature of polarized epithelial cells is the ability to maintain specific biochemical composition apical and basolateral plasma membrane domains while selectively allowing transport proteins lipids from one pole opposite by transcytosis. The small GTPase, rab17, a member rab family regulators intracellular transport, specifically induced during cell polarization in developing kidney. We here examined its distribution function both nonpolarized cells. By confocal immunofluorescence...
Vectors based on herpes simplex virus type 1 (HSV-1) show promise for gene transfer into mammalian cells because of their wide host range, efficient infection and ability to deliver genes nondividing cells. Defective HSV-1 vectors, or amplicons, are plasmid vectors which unable propagate own but contain specific sequences that, in the presence helper virus, support DNA replication subsequent packaging particles. We compared three replication-incompetent mutants (KOS strain 5dll.2, 17 D30EBA,...
The nucleotide sequence of the yeast pyruvate carboxylase gene has been determined from a cloned fragment genomic DNA. deduced translation product codes for polypeptide 1178 amino acids, having calculated molecular weight 130,100. protein shows strong homology to specific regions other biotin carboxylases, lipoamide transferases, and carbamyl phosphate synthetases. homologous suggest presence three subsites in enzyme: attachment site, keto acid-binding an ATP-binding site. Partial...
Tau pathology, associated with Alzheimer's disease, is characterized by the presence of phosphorylated and aggregated tau. Phosphorylation tau takes place mainly in vicinity tubulin-binding region molecule its self aggregation also mediated via this region. phosphorylation have been related conformational changes protein. These could be regulated chaperones such as heat shock proteins, since one these, protein 90 (Hsp90), has already described a putative tau-binding In work, we confirmed...
One of the promising strategies for neural repair therapies is transplantation olfactory ensheathing cells (OECs) which are glial system. We evaluated effects curcumin on behaviour mouse OECs to determine if it could be use further enhance therapeutic potential OECs. Curcumin, a natural polyphenol compound found in spice turmeric, known its anti-cancer properties at doses over 10 µM, and often 50 exerts cancer part by activation MAP kinases. In contrast, we that low-dose (0.5 µM) applied...
Mitochondrial alterations are detected in most neurodegenerative disorders and may contribute to the dysfunction demise of neuronal cells. Because glycogen synthase kinase-3 (GSK-3) is considered be a critical factor regulating cell survival death, we studied effects modulating GSK-3 activity cultured neurons treated with mitochondrial inhibitor, rotenone. Interestingly, chronic inhibition protects against rotenone-induced apoptosis In an attempt elucidate molecular mechanisms underlying...
Friedreich's ataxia (FA) is the most common recessive ataxia, affecting 1-2 in 50,000 Caucasians, and there currently no effective cure or treatment. FA results from a deficiency of mitochondrial protein frataxin brought about by repeat expansion intron 1 FRDA gene. The main areas affected are central nervous system (particularly spinocerebellar system) cardiac tissue. Therapies aimed at alleviating neurological degeneration have proved unsuccessful to date. Here, we describe construction...
It has been extensively described that neuronal differentiation involves the signalling through neurotrophin receptors to a Ras‐dependent mitogen‐activated protein kinase (MAPK) cascade. However, pathways from other neuritogenic factors have not well established. reported cAMP may activate (PKA), and it shown PKA‐mediated stimulation of MAPK pathway regulates only neuritogenesis but also survival. extracellular regulated kinases (ERKs) mediated are sufficient explain all processes which...
Abstract Gene transfer is a powerful tool for functional gene analysis in human cells. In this respect, there need to develop experimental models that involve homogeneous cultures of neuron‐like cells susceptible transduction and are easy handle. Here we describe an optimized reproducible procedure differentiate SH‐SY5Y neuroblastoma into population The fully differentiated postmitotic resemble primary cultured neurons terms their cytoskeletal polarity. Notably, far more by herpes simplex...
There is currently no effective treatment for Friedreich's ataxia (FA), the most common of hereditary ataxias. The disease caused by mutations in FRDA that drastically reduce expression levels mitochondrial protein frataxin. In FA animal models, a key difficulty obtaining precise frataxin appropriate tissues to provoke pathology without early lethality. To develop strategies circumvent these problems, conditional transgenic mice have been generated. We now show can be eliminated neurons from...
Abstract: The recent finding that several point mutations in the gene encoding for microtubule‐binding protein tau correlate with neurological disorders has heightened interest mechanisms of destabilization this protein. In study functional consequences mutation R406W on interaction microtubules have been analyzed. Mutated is less phosphorylated than its normal counterpart at serines 396 and 404. Furthermore, mutant unable to bind microtubules, and, as a consequence, assembled after...