A5047777069- Photoreceptor and optogenetics research
- Neurogenesis and neuroplasticity mechanisms
- Neural dynamics and brain function
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Nerve injury and regeneration
- RNA and protein synthesis mechanisms
- Neuroscience and Neural Engineering
- Cell Image Analysis Techniques
- Neuroscience and Neuropharmacology Research
- Research Data Management Practices
- Functional Brain Connectivity Studies
- Chemical Synthesis and Analysis
- Light effects on plants
- Scientific Computing and Data Management
- RNA Interference and Gene Delivery
- Memory and Neural Mechanisms
- Optical Coherence Tomography Applications
- Zebrafish Biomedical Research Applications
- Advanced Electron Microscopy Techniques and Applications
- Neurobiology and Insect Physiology Research
- Click Chemistry and Applications
- Protein Degradation and Inhibitors
- RNA Research and Splicing
The Nature Conservancy
2015-2024
Springer Nature (United States)
2020-2021
Columbia University
2009-2014
Columbia University Irving Medical Center
2011
Consejo Superior de Investigaciones Científicas
2006
Universidad Autónoma de Madrid
2006
Centro de Biología Molecular Severo Ochoa
2006
The ability to prospectively isolate adult neural stem cells and their progeny is crucial study biology therapeutic potential. Stem in mammalian neurogenic niches are a subset of astrocytes. A major limitation the field has been inability distinguish cell astrocytes from niche Here, we show that epidermal growth factor receptor (EGFR)-positive subventricular-zone (SVZ) activated eliminated by antimitotic treatment. We developed simple strategy simultaneously purify at different stages SVZ...
Abstract We describe a novel protocol for three-dimensional culturing of olfactory ensheathing cells (OECs), which can be used to understand how OECs interact with other in three dimensions. Transplantation is being trialled repair the paralysed spinal cord, promising but variable results and thus therapy needs improving. To date, studies OEC behaviour multicellular environment have been hampered by lack suitable cell culture models. Here, we exploit floating liquid marble, droplet coated...
<ns4:p>Software is as integral a research paper, monograph, or dataset in terms of facilitating the full understanding and dissemination research. This article provides broadly applicable guidance on software citation for communities institutions publishing academic journals conference proceedings. We expect those to produce versions this document with examples styles that are appropriate their intended audience. (and community-specific versions) aimed at authors citing software, including...
The molecular mechanisms used by olfactory ensheathing cells (OECs) to promote repair in the damaged adult mammalian CNS remain unknown. Thus, we microarrays analyze three OEC populations with different capacities axonal regeneration cultured rat retinal neurons. Gene expression “long-term OECs” that do not stimulate outgrowth was compared of “primary cells” and immortalized cell line TEG3. In this way, identified a number candidate genes might play role promoting regeneration. Among these...
We investigated the expression and role of dopamine receptor 3 (D3R) in postnatal mouse subventricular zone (SVZ). In situ hybridization detected selective D3R mRNA SVZ. Fluorescence activated cell sorting (FACS) adult SVZ subtypes using hGFAP-GFP Dcx-GFP mice showed that transit amplifying progenitor cells niche astrocytes expressed whereas stem cell-like neuroblasts did not. To determine D3R's neurogenesis, we administered U-99194A, a preferential antagonist, bromodeoxyuridine mice. vivo...
One of the promising strategies for neural repair therapies is transplantation olfactory ensheathing cells (OECs) which are glial system. We evaluated effects curcumin on behaviour mouse OECs to determine if it could be use further enhance therapeutic potential OECs. Curcumin, a natural polyphenol compound found in spice turmeric, known its anti-cancer properties at doses over 10 µM, and often 50 exerts cancer part by activation MAP kinases. In contrast, we that low-dose (0.5 µM) applied...
Immortalized cell lines of olfactory ensheathing glia (OEG) that maintain the proregenerative properties primary cultures provide an unlimited source OEG for both basic and applied studies. Indeed, one specific immortalized rat clonal line (TEG3) proved to be as good in promoting neuritogenesis axon regeneration culture models. Thus, we examined capacity TEG3 promote axonal repair animal model spinal cord injury, dorsal column crush. cells can acquire astrocyte-like or Schwann cell-like...
Olfactory ensheathing glia (OEG) cells are known to facilitate repair following axotomy of adult neurons, although the molecular mechanisms involved not fully understood. We previously identified plasminogen activator inhibitor-1 (PAI-1), proteinase-activated receptor-1 (PAR-1), and thrombomodulin (TM) as candidates regulate rat OEG-dependent axonal regeneration. In this study, we have validated involvement these proteins in promoting regeneration by immortalized human OEGs. studied effect...
<ns3:p>Software is as integral a research paper, monograph, or dataset in terms of facilitating the full understanding and dissemination research. This article provides broadly applicable guidance on software citation for communities institutions publishing academic journals conference proceedings. We expect those to produce versions this document with examples styles that are appropriate their intended audience. (and community-specific versions) aimed at authors citing software, including...