Herpes simplex virus type 1 (HSV-1) plasmid vectors have promise for genetic intervention in the brain, but several problems caused by helper compromised their utility. To develop a virus-free packaging system these vectors, DNA cleavage/packaging signals were deleted from set of cosmids that represents HSV-1 genome. Following cotransfection into cells, this modified cosmid supported replication and vector DNA. However, absence signals, genome was not packaged, consequently stocks free...

Vectors based on herpes simplex virus type 1 (HSV-1) show promise for gene transfer into mammalian cells because of their wide host range, efficient infection and ability to deliver genes nondividing cells. Defective HSV-1 vectors, or amplicons, are plasmid vectors which unable propagate own but contain specific sequences that, in the presence helper virus, support DNA replication subsequent packaging particles. We compared three replication-incompetent mutants (KOS strain 5dll.2, 17 D30EBA,...

Abstract: A defective herpes simplex virus type one (HSV‐1) vector that contains a 6.8‐kb fragment of the rat tyrosine hydroxylase promoter (pTHlac‐7kb) was examined for its capability to target catecholaminergic cell type‐specific expression in CNS. Cell assessed by comparison with control (pHSVlac) uses HSV‐1 immediate early 4/5 support multiple types. In initial experiments comparing and noncatecholaminergic lines, pTHlac‐7kb supported seven‐ 20‐fold increase reporter gene lines. Four...

Understanding the factors that influence human papillomavirus (HPV) vaccination uptake is critically important to design of effective programmes. In Switzerland, HPV (≥1 dose) by age 16 years among women ranges from 31% 80% across 26 cantons (states). Our objective was identify are associated with spatial variation in uptake. We used cross-sectional data Swiss National Vaccination Coverage Survey 2009-2016 on status 14-17-year-old girls, their municipality residence and nationality for 21...

To estimate effectiveness of tickborne encephalitis (TBE) vaccination by time interval (<5, 5-10 and 10+years) postvaccination.A retrospective, matched case-control study PARTICIPANTS: Cases-all adult (age 18-79) TBE cases in Switzerland reported via the national mandatory disease reporting surveillance system from 2006 to 2020 (final n=1868). Controls-community controls a database randomly selected adults participating 2018 cross-sectional n=4625).For controls, number vaccine doses received...

Overall incidence and geographic range of Tick-borne Encephalitis (TBE), a vaccine preventable infection, have steadily increased in Switzerland over the last 50 years. While fully subsidized vaccination has been recommended many areas for well decade, coverage variables associated with compliance among Swiss adults are poorly understood. In 2018 we conducted national, cross-sectional survey cards evaluating TBE (18-79) Switzerland. Nationwide was 41.7% (range 14.3% to 60.3%) 1 dose 32.9%...

Tick-borne Encephalitis (TBE) is a severe disease of the Central Nervous System (CNS) caused by tick-borne encephalitis virus (TBEV). The generation protective immunity after TBEV infection or TBE vaccination relies on integrated responses many distinct cell types at physical locations. While long-lasting memory immune responses, in particular, form basis for correlates protection against diseases, these have not yet been clearly defined TBE. This review addresses control and to vaccination....

BackgroundTick-borne encephalitis (TBE) is a severe, vaccine-preventable viral infection of the central nervous system. Symptoms are generally milder in children and adolescents than adults, though severe disease does occur. A better understanding burden duration vaccine-mediated protection important for vaccination recommendations.AimTo estimate TBE coverage, severity vaccine effectiveness (VE) among individuals aged 0-17 years Switzerland.MethodsVaccination coverage between 2005 2022 was...

Objectives. We described the results from Swiss National Vaccination Coverage Survey (SNVCS) 2005–2007, a survey designed to monitor immunization coverage of children and adolescents residing in Switzerland each canton within three-year period. Methods. The SNVCS is cross-sectional using two-stage sampling design targeting aged 2, 8, 16 years. Families selected were contacted by mail telephone. was determined via vaccination cards or summary forms. Results. A total 25 out 26 cantons...

Streptococcus pneumoniae , or pneumococcus, is a common, opportunistic pathogen which can cause severe disease, particularly in adults 65+. In Switzerland, vaccination recommended for children under 5 and with health predispositions; of healthy 65+ not recommended. 2020 we conducted nationwide, cross-sectional survey records to evaluate pneumococcal coverage factors affecting uptake among 18–85. We found that nationwide was 4.5% without significant regional differences. Coverage comparable...

Objectives: As no data are available regarding the influenza vaccination status of Swiss healthcare workers (HCW) in ambulatory setting, this study aims to investigate their behaviours. Methods: We conducted an online survey using a four-item, semi-structured questionnaire assess HCWs coverage and behaviour. Associations between status, age language as well recommendation behaviour reasons for were assessed descriptive statistics logistic regression analyses. Results: Of 1057 completed...

Pneumococcal conjugate vaccines (PCVs) have lowered the incidence of invasive pneumococcal disease (IPD) worldwide. However, influence regional vaccine uptake differences on changing epidemiology IPD remains unclear. We aimed to examine overall impact both seven- and 13-valent PCVs (PCV7 PCV13) in Switzerland. Three-year periods from 2005–2010 2011–2019 were considered, respectively, as (early late) PCV7 eras (early, mid PCV13 eras. Vaccine coverage was estimated a nationwide survey...

Genetic intervention is becoming increasingly useful in elucidating the molecular basis of various biological processes, including those brain. Many genes have now been isolated which encode key regulatory molecules such as signal transduction components or transcription factors, but relatively little known about their effects on neuronal physiology higher order brain function. Gene transfer experiments offer potential to study highly specific alterations gene products normal environment. In...

In Switzerland, the implementation of national health and prevention strategies is regulated at cantonal level. To date, no in-depth inventory outlining activities legal frameworks for influenza vaccination exists.To assess during 2019/20 season across all 26 Swiss cantons.Survey using a structured closed-ended questionnaire.The activities' range cantons broad from 11 in canton Vaud to one Grisons Jura. French/Italian-speaking conduct 1.5 times more on average than German-speaking cantons....

Seasonal influenza may cause serious illness, especially in high-risk populations such as older adults and individuals suffering from non-communicable diseases (NCD) be prevented by a vaccination. However, an assessment of the impact Swiss legal frameworks number health activities on vaccination coverage population at cantonal level is lacking.Two participating healthcare insurers sent out 25,000 semi-structured questionnaires to their subscribers aged 60-85 five cantons selected according...

The incidence of influenza and influenza-like illnesses in Switzerland is generally high. Although related direct medical costs can be substantial, especially if hospitalisations occur, several studies suggested that indirect due to the loss productivity may represent an even higher economic burden. aim this study was assess arising from lost Switzerland.Analyses were based on data collected 2016 2017 by Swiss Sentinel Surveillance Network Federal Office Public Health (SFOPH). available...

The goal of this study was to evaluate timeliness Tick-borne Encephalitis vaccination uptake among adults in Switzerland. In cross-sectional survey, we collected records from randomly selected 18–79 throughout Of 4,626 participants, data individuals receiving at least 1 TBE (n = 1875) were evaluated. We determined year and age first vaccine compliance, evaluating dose timeliness. Participants considered “on time” if they received doses according the recommended schedule ± a 15% tolerance...