A5042037323- Cancer-related Molecular Pathways
- Caveolin-1 and cellular processes
- Microbial Metabolites in Food Biotechnology
- Sarcoma Diagnosis and Treatment
- Biofuel production and bioconversion
- Cancer, Lipids, and Metabolism
- PARP inhibition in cancer therapy
- Cell death mechanisms and regulation
- Enzyme Production and Characterization
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- RNA Research and Splicing
- Fungal and yeast genetics research
- RNA modifications and cancer
- Gut microbiota and health
- Genomics and Chromatin Dynamics
- Molecular Biology Techniques and Applications
- Biomarkers in Disease Mechanisms
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Retinal Diseases and Treatments
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Glycosylation and Glycoproteins Research
- Metal complexes synthesis and properties
Comprehensive Blood & Cancer Center
2023
U-M Rogel Cancer Center
2023
Georgetown University
2010-2022
Georgetown University Medical Center
2010-2022
Molecular Oncology (United States)
2013
CIBBIM-Nanomedicine
2010
St. Jude Children's Research Hospital
2010
Institut d'Investigació Biomédica de Bellvitge
2010
Georgetown Lombardi Comprehensive Cancer Center
2010
Centre National de la Recherche Scientifique
2009
Pigment epithelium-derived factor (PEDF), a neurite-promoting factor, has an amino acid primary structure that is related to members of the serine protease inhibitor (serpin) family. Controlled proteolysis native PEDF (50 kDa) with either trypsin, chymotrypsin, elastase, or subtilisin yields in each case one major limited product 46 kDa as analyzed by SDS-polyacrylamide gel electrophoresis. N-terminal sequence analysis isolated 46-kDa products indicates favored cleavage region located toward...
Gastrin releasing peptide is mitogenic for mouse Swiss 3T3 fibroblasts and certain human small cell lung carcinoma (SCLC) cells but not Balb/c fibroblasts. To identify new molecules associated with the gastrin peptide-responsive phenotype, clones isolated from a differential cDNA library between were used to screen their expression in SCLC lines. Using this approach, we have characterized encoding novel protein. This protein putative transmembrane belonging epidermal growth factor-like...
Abstract Tumors of the Ewing's sarcoma family (ESFT), such as (EWS) and primitive neuroectodermal tumors (PNET), are highly aggressive malignancies predominantly affecting children young adults. ESFT express chimeric transcription factors encoded by hybrid genes fusing EWS gene with several ETS genes, most commonly FLI-1. EWS/FLI-1 proteins responsible for malignant phenotype ESFT, but only few their transcriptional targets known. Using antisense short hairpin RNA–mediated expression...
The coding region of a human beta-polymerase cDNA, predicting 335 amino acid protein, was subcloned in the Escherichia coli expression plasmid pRC23. After induction transformed cells, crude soluble extract found to contain new protein immunoreactive with antibody and corresponding size deduced from cDNA. This purified yield 1-2 mg/50 g cells. recombinant had about same DNA polymerase specific activity as mammalian tissues, template-primer specificity immunological properties were similar...
Abstract Protein kinases represent promising anticancer drug targets. We describe here the meriolins, a new family of inhibitors cyclin-dependent (CDK). Meriolins chemical structural hybrid between meridianins and variolins, two families kinase extracted from various marine invertebrates. Variolin B is currently in preclinical evaluation as an antitumor agent. A selectivity study done on 32 showed that, compared with variolin B, meriolins display enhanced specificity toward CDKs, marked...
The H-ras gene of the BALB murine sarcoma virus (BALB-MSV) was placed under transcriptional control tightly regulated PL promoter bacteriophage lambda in expression vectors pEV-vrf-1 and pRC23. Upon derepression promoter, large amounts (10-20% total cellular protein) product p21 are synthesized Escherichia coli. We constructed three vectors, designated pJCL-H5, pJCL-E30, pJCL-33. pJCL-H5 directs synthesis p21, a fusion protein whose four amino-terminal residues replaced by eight amino acids...
Pharmacological inhibitors of cyclin-dependent kinases (CDKs) have a wide therapeutic potential. Among the CDK currently under clinical trials, 2,6,9-trisubstituted purine (R)-roscovitine displays rather high selectivity, low toxicity, and promising antitumor activity. In an effort to improve this structure, we synthesized several bioisosteres roscovitine. Surprisingly, one them, pyrazolo[1,5-a]-1,3,5-triazine 7a (N-&-N1, GP0210), displayed significantly higher potency, compared...
Pigment epithelium-derived factor (PEDF) is a neurotrophic protein present in low amounts conditioned medium of cultured fetal human retinal pigment epithelial cells. Recently, the PEDF cDNA has been cloned from library, and its derived amino acid sequence identified it as member serine protease inhibitor (serpin) supergene family (Steele, F. R., Chader, G. J., Johnson, L. V., Tombran-Tink, J. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 1526-1530). We have prepared recombinant expression...
Pigment epithelium-derived factor (PEDF) is a neurotrophic protein and member of the serine protease inhibitor superfamily. Here we describe identification PEDF in bovine eyes optimization its purification from this natural source. We have developed polyclonal antibody to recombinant human PEDF, Ab-rPEDF, that immunoreacts specific, sensitive, linear fashion with protein, furthermore, blocks activity. show Ab-rPEDF specifically recognizes 49,500-M(r) polypeptide on Western transfers wash...
Abstract The Ewing's sarcoma family of tumors (ESFT) comprises several well-characterized malignant neoplasms with particularly aggressive behavior. Despite recent progress in the use multimodal therapeutic approaches and local control measures, a substantial proportion patients die because disease progression. Furthermore, this outcome has not changed significantly over last 15 to 20 years. Consequently, new, more effective options are sorely needed for treatment ESFT. Because ESFT cells...
Abstract Metastasis is the final stage of tumor progression and thought to be responsible for up 90% deaths associated with solid tumors. Caveolin-1 (CAV1) regulates multiple cancer-associated processes related malignant progression. In present study, we tested hypothesis that CAV1 modulates metastatic ability cells from Ewing's sarcoma family tumors (ESFT). First, analyzed expression by immunostaining a tissue microarray containing 43 paraffin-embedded ESFT known EWS translocations. Even...
beta-Glucanase present in cell-free extracts from Candida utilis was isolated and purified 562-fold by procedures that include adsorption on DEAE-Sephadex A-50 filtration through columns of Sephadex G-50, G-100 G-200, Bio-Gel P-10, Concanavalin A-Sepharose 4B. The enzyme appeared homogeneous polyacrylamide-gel electrophoresis ultracentrifugation studies (S20,w = 1.74S). behaved as an acidic glycoprotein (pI4.1) with 68% carbohydrate a high content amino acids. mol.wt. estimated to be 20000...
Despite increased use of early detection methods and more aggressive treatment strategies, the worldwide incidence colorectal cancer is still on rise. Consequently, it remains urgent to identify novel agents with enhanced efficacy in prevention and/or therapeutic protocols. Our studies focused Plumbagin, a natural phytochemical that showed promising results against other tumor types, determine its effectiveness blocking proliferation survival colon cells experimental protocols mimicking...
Abstract Purpose: To show the efficacy of targeting EWS/FLI-1 expression with a combination specific antisense oligonucleotides and rapamycin for control Ewing's sarcoma (EWS) cell proliferation in vitro treatment mouse tumor xenografts vivo. Experimental Design: EWS cells were simultaneously exposed to EWS/FLI-1–specific various time periods. After treatment, following end points monitored evaluated: levels protein, proliferation, cycle distribution, apoptotic death, caspase activation,...