A5101698704- Wound Healing and Treatments
- Pharmacological Effects of Natural Compounds
- Ovarian function and disorders
- Retinal Diseases and Treatments
- Inflammatory Bowel Disease
- RNA modifications and cancer
- Diabetic Foot Ulcer Assessment and Management
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Connexins and lens biology
- Proteoglycans and glycosaminoglycans research
- Electrospun Nanofibers in Biomedical Applications
- Immune Response and Inflammation
- Adipose Tissue and Metabolism
- Retinal Development and Disorders
- Peptidase Inhibition and Analysis
- Reproductive Biology and Fertility
- Ginseng Biological Effects and Applications
- Clusterin in disease pathology
- Barrier Structure and Function Studies
- Curcumin's Biomedical Applications
- Pressure Ulcer Prevention and Management
- Cancer Mechanisms and Therapy
- Biochemical and Structural Characterization
Mudanjiang Medical University
2022-2024
First Affiliated Hospital of Jiangxi Medical College
2024
Nanchang University
2019-2024
Fujian Medical University
2022-2023
Yantai University
2022-2023
Medical University of South Carolina
2020-2023
Guangzhou Center for Disease Control and Prevention
2023
Jinan Central Hospital
2023
Shandong First Medical University
2023
Union Hospital
2022
The application of gelatin hydrogels is hampered by their poor mechanical properties, which are unfavorable for debridement and diabetic wound healing. Herein, bovine serum albumin (BSA)-bridged biopotentiated bone marrow mesenchymal stem cell (BMSC)-derived nanovesicles (NV), named [email protected], were fabricated reinforcement with genipin BSA in a one-pot, low-cost green approach based on amide bonding-guided crosslinking. Genipin introduction endowed the crosslinking weak properties...
3T3-L1 preadipocytes have been shown to exhibit a transient increase in poly(ADP-ribose) polymerase (PARP) protein and activity, as well an association of PARP with DNA α, within 12−24 h exposure inducers differentiation, whereas cells expressing antisense RNA showed no are unable complete the round replication required for differentiation into adipocytes. The role differentiation-linked has now further clarified at both cellular enzymological levels. Flow cytometric analysis revealed that...
Diabetic wound healing remains a worldwide challenge for both clinicians and researchers. The high expression of matrix metalloproteinase 9 (MMP9) inflammatory response are indicative poor diabetic healing. H8, curcumin analogue, is able to treat diabetes anti-inflammatory, our pretest showed that it has the potential However, H8 highly expressed in organs such as liver kidney, resulting its unfocused use targeting. (These data were not published, see Table S1 Supporting Information.)...
Stem cell injection is an effective approach for treating diabetic wounds; however, shear stress during injections can negatively affect their stemness and growth. Cell-laden porous microspheres provide shelter bone mesenchymal stem cells (BMSC). Herein, curcumin-loaded flower-like (CFPM) are designed by combining phase inversion emulsification with thermally induced separation-guided four-arm poly (l-lactic acid) (B-PLLA). Notably, the CFPM shows a well-defined surface topography inner...
Pigment epithelium-derived factor (PEDF) is a neurotrophic protein and member of the serine protease inhibitor superfamily. Here we describe identification PEDF in bovine eyes optimization its purification from this natural source. We have developed polyclonal antibody to recombinant human PEDF, Ab-rPEDF, that immunoreacts specific, sensitive, linear fashion with protein, furthermore, blocks activity. show Ab-rPEDF specifically recognizes 49,500-M(r) polypeptide on Western transfers wash...
Per- and polyfluoroalkyl substances (PFAS) can disrupt liver homeostasis. Studies have shown that a single exposure to PFAS may provoke abnormal function; however, few studies investigated the overall effect of mixtures. We aimed investigate associations between mixtures function indices explore relevant mechanisms. This study included 278 adult males from Guangzhou, China. Serum metabolite profiles were analyzed using untargeted metabolomics. applied weighted quantile sum (WQS) regression...
Sepsis-associated encephalopathy (SAE) is characterized by acute and diffuse brain dysfunction correlates with long-term cognitive impairments no targeted therapy. We used a mouse model of sepsis-related impairment to examine the role lncRNA nuclear enriched abundant transcript 1 (Neat1) in SAE. observed that Neat1 expression was increased neuronal cells from septic mice it directly interacts hemoglobin subunit beta (Hbb), preventing its degradation. The Neat1/Hbb axis suppressed...
Abstract Stress has been shown to enhance pain sensitivity resulting in stress-induced hyperalgesia. However, the underlying mechanisms have yet be elucidated. Using single-prolonged stress combined with Complete Freund’s Adjuvant injection model, we explored reciprocal regulatory relationship between neurons and microglia, which is critical for maintenance of posttraumatic disorder (PTSD)-induced In our assay, significant mechanical allodynia was observed. Additionally, activated spinal...
Abstract We have identified and purified a multiprotein form of DNA polymerase from the murine mammary carcinoma cell line (FM3A) using series centrifugation, polyethylene glycol precipitation, ion‐exchange chromatography steps. Proteins enzymatic activities associated with this mouse include polymerases α δ, primase, proliferating nuclear antigen (PCNA), ligase I, helicase, topoisomerases I II. The sedimentation coefficient is 17S, as determined by sucrose density gradient analysis....
Colchicine (COL) is a well-known plant alkaloid long used for medical purposes due to the selective anti-inflammatory effect on acute gouty arthritis. It also kind of mitosis toxin with strong inhibitory effects cell division and therefore being applied treatment various cancers. However, this product shows variety adverse that are significantly correlated dosage have attracted much attention. For first time, present work obtained new insight into gastrointestinal toxicity colchicine...
Multiple myeloma (MM) is an incurable plasma cell malignancy. Aberrant activation of the Hedgehog (Hh) and NF-κB signaling pathways observed in MM plays a pivotal role development by promoting growth, survival, drug resistance. In this study, we found that Sonic Hh (SHh) ligand bone marrow microenvironment responsible for enhancement activity lines NCI-H929 U266. Notably, discovered regulates through its classical pathway (SHh/PTCH1/SMO/GLI1) cells. Meanwhile, non-classical SMO recruitment...
Sepsis is a life-threatening systemic disease with severe microvascular dysfunction. Pericytes preserve vascular homeostasis. To our knowledge, the potential roles of microRNAs in sepsis-induced pericyte dysfunction have not been explored.We determined lung expression miR-145a cecal ligation and puncture (CLP)-induced sepsis. Mouse pericytes were isolated transfected mimic, followed by stimulation lipopolysaccharide (LPS). We measured inflammatory cytokine levels. assess functions vivo, we...
CD44 is a transmembrane glycoprotein expressed in various tissues including the skin. Previous studies indicated that required for epidermal permeability barrier homeostasis and keratinocyte differentiation. Yet, while some have demonstrated critical development of inflammation, others shown not essential cutaneous inflammation. In this study, we evaluated changes expression variety skin inflammatory models determined whether Inflammatory responses were compared KO versus wild-type mice...
N-(Cyanoacetyl)-L-phenylalanine (compound 1) and N-(3-chloropropionyl)-L-phenylalanine 2) were studied as the first peptidic mechanism-based inactivators (suicide substrates) for zinc protease carboxypeptidase A (CPA). crucial deprotonation on methylene alpha to amide carbonyl of 1 2 has been suggested lead transient formation a ketenimine an alpha, beta-unsaturated amide, respectively. Subsequently, it is proposed that these key intermediates trap active site nucleophile, resulting in...
The aim of this study was to observe the therapeutic effect curcumin chitosan microspheres (CCM) on ulcerative colitis (UC) in rats. Rat UC model prepared by 2,4,6-trinitrobenzenesulfonic acid (TNBS) method. All animals were divided into blank control group (BCG), (MCG), mesalazine enteric-coated tablets (MECT), microsphere low (CCML), medium (CCMM) and high dose groups (CCMH). Starting from third day after establishment, rats intra-gastrically administered for 10 days. On 14th experiment,...
Ulcerative colitis (UC) is a commonly recurrent inflammatory bowel disease. T helper 17 (Th17)/regulatory (Treg) cell balance plays an essential role in UC progression. However, it unknown whether curcumin chitosan microspheres (CCM) regulate the Th17/Treg balance.