A5083888813- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Photoreceptor and optogenetics research
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Advanced Memory and Neural Computing
- Functional Brain Connectivity Studies
- Marine Invertebrate Physiology and Ecology
- EEG and Brain-Computer Interfaces
- Neurogenesis and neuroplasticity mechanisms
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Axon Guidance and Neuronal Signaling
- Neuroethics, Human Enhancement, Biomedical Innovations
- Receptor Mechanisms and Signaling
- Neurobiology and Insect Physiology Research
- Photochromic and Fluorescence Chemistry
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Visual perception and processing mechanisms
- Single-cell and spatial transcriptomics
- Neural Networks and Applications
- Digital Holography and Microscopy
- Ion channel regulation and function
Columbia University
2016-2025
Columbia University Irving Medical Center
2024
Neurotech (United States)
2016-2023
Marine Biological Laboratory
2019-2023
Donostia International Physics Center
2020-2022
Howard Hughes Medical Institute
2004-2013
University of Southern California
2013
Newcastle University
2009
Massachusetts Institute of Technology
2006
Instituto Cajal
2005
How can neural activity propagate through cortical networks built with weak, stochastic synapses? We find precise repetitions of spontaneous patterns synaptic inputs in neocortical neurons vivo and vitro. These repeat after minutes, maintaining millisecond accuracy. Calcium imaging slices reveals reactivation sequences cells during the occurrence repeated intracellular patterns. The drifts time, engaging different cells. Sequences active have distinct spatial structures are same order over...
GABAergic interneurons play a major role in the function of mammalian neocortex, but their circuit connectivity is still poorly understood. We used two-photon RuBi-Glutamate uncaging to optically map how largest population cortical interneurons, parvalbumin-positive cells (PV+), are connected pyramidal (PCs) mouse neocortex. found locally dense from PV+ onto PCs across areas and layers. In many experiments, all nearby were every local PC sampled. agreement with this, we no evidence for...
The mammalian neocortex consists of a mosaic columnar units whose development is poorly understood. Optical recordings brain slices labeled with the fluorescent calcium indicator fura-2 revealed that neonatal rat cortex was partitioned into distinct domains spontaneously coactive neurons. In tangential slices, these were 50 to 120 micrometers in diameter; coronal they spanned several cortical layers and resembled columns found adult cortex. developing somatosensory cortex, smaller than,...
Calcium imaging for observing spiking activity from large populations of neurons are quickly gaining popularity. While the raw data fluorescence movies, underlying spike trains interest. This work presents a fast non-negative deconvolution filter to infer approximately most likely train each neuron, given observations. algorithm outperforms optimal linear (Wiener filtering) on both simulated and biological data. The performance gains come restricting inferred be positive (using an...
The function of dendritic spines, postsynaptic sites excitatory input in the mammalian central nervous system (CNS), is still not well understood. Although changes spine morphology may mediate synaptic plasticity, extent basal motility and its regulation remains controversial. We investigated three principal neurons mouse CNS: cerebellar Purkinje cells, cortical hippocampal pyramidal neurons. Motility was assayed with time-lapse imaging by using two-photon microscopy green fluorescent...
The location and trajectory of seizure activity is great importance, yet our ability to map such remains primitive. Recently, the development multi-electrode arrays for use in humans has provided new levels temporal spatial resolution recording seizures. Here, we show that there a sharp delineation between areas showing intense, hypersynchronous firing indicative recruitment seizure, adjacent territories where only low-level, unstructured firing. Thus, core territory recruited neurons...
The role of astrocytes in neuronal function has received increasing recognition, but disagreement remains about their at the circuit level. Here we use vivo two-photon calcium imaging neocortical while monitoring activity state local electrophysiologically and optically. We find that astrocytic precedes spontaneous shifts to slow-oscillation-dominated state, a rhythm characterized by synchronized firing important for sleep memory. Further, show optogenetic activation switches this...