A5041323310- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Advanced Fluorescence Microscopy Techniques
- EEG and Brain-Computer Interfaces
- Receptor Mechanisms and Signaling
- Memory and Neural Mechanisms
- Visual perception and processing mechanisms
- Neurogenesis and neuroplasticity mechanisms
- Retinal Development and Disorders
- Optical Coherence Tomography Applications
- Cell Image Analysis Techniques
- Adaptive optics and wavefront sensing
- Advanced Memory and Neural Computing
- Neural Networks and Applications
- Cellular Automata and Applications
- Genetics and Neurodevelopmental Disorders
- Pain Management and Placebo Effect
- Neurobiology and Insect Physiology Research
- Hemispheric Asymmetry in Neuroscience
- Traumatic Brain Injury Research
- Transcranial Magnetic Stimulation Studies
- Pain Mechanisms and Treatments
- Photoacoustic and Ultrasonic Imaging
University of Oxford
2019-2025
University College London
2013-2024
Wolfson Foundation
2012-2024
Norwegian University of Science and Technology
2024
European Research Council
2022
Biotechnology and Biological Sciences Research Council
2022
Wellcome Trust
2022
Royal Society
2022
Pediatrics and Genetics
2022
Columbia University
2009-2012
GABAergic interneurons play a major role in the function of mammalian neocortex, but their circuit connectivity is still poorly understood. We used two-photon RuBi-Glutamate uncaging to optically map how largest population cortical interneurons, parvalbumin-positive cells (PV+), are connected pyramidal (PCs) mouse neocortex. found locally dense from PV+ onto PCs across areas and layers. In many experiments, all nearby were every local PC sampled. agreement with this, we no evidence for...
Calcium imaging for observing spiking activity from large populations of neurons are quickly gaining popularity. While the raw data fluorescence movies, underlying spike trains interest. This work presents a fast non-negative deconvolution filter to infer approximately most likely train each neuron, given observations. algorithm outperforms optimal linear (Wiener filtering) on both simulated and biological data. The performance gains come restricting inferred be positive (using an...
Many theories of brain function propose that activity in sparse subsets neurons underlies perception and action. To place a lower bound on the amount neural can be perceived, we used an all-optical approach to drive behaviour with targeted two-photon optogenetic activation small ensembles L2/3 pyramidal mouse barrel cortex while simultaneously recording local network calcium imaging. By precisely titrating number stimulated, demonstrate for cortical is ~14 neurons. We find steep sigmoidal...
Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CLCX), this has yet be fully accomplished. The CLCX is generally considered comprise two major subdivisions, (CL) and dorsal endopiriform nucleus (DEn), regional boundaries these areas debated. To address this, we conducted multifaceted analysis of fiber- cytoarchitecture, genetic marker expression, connectivity using mice both sexes, create comprehensive...
Abstract The mechanistic link between neural circuit activity and behavior remains unclear. While manipulating cortical can bias certain behaviors elicit artificial percepts, some tasks still be solved when cortex is silenced or removed. Here, mice were trained to perform a visual detection task during which we selectively targeted groups of visually responsive co-tuned neurons in L2/3 primary (V1) for two-photon photostimulation. influence photostimulation was conditional on two key...
Abstract Brains are composed of anatomically and functionally distinct regions performing specialized tasks, but do not operate in isolation. Orchestration complex behaviors requires communication between brain regions, how neural dynamics organized to facilitate reliable transmission is well understood. Here we studied this process directly by generating activity that propagates drives behavior, assessing populations sensory cortex cooperate transmit information. We achieved imaging two...
Abstract The resolution and contrast of microscope imaging is often affected by aberrations introduced imperfect optical systems inhomogeneous refractive structures in specimens. Adaptive optics (AO) compensates these restores diffraction limited performance. A wide range AO solutions have been introduced, tailored to a specific type or application. Until now, universal solution – one that can be readily transferred between modalities has not deployed. We propose versatile fast aberration...
Neocortical GABAergic interneurons have important roles in the normal and pathological states of circuit. Recent work has revealed that somatostatin-positive (SOM) parvalbumin-positive (PV) connect promiscuously to pyramidal cells (PCs). We investigated whether Peters' rule, is, spatial overlap axons dendrites, could explain this unspecific connectivity. reconstructed morphologies P11–17 mouse SOM PV their PC targets, performed Monte Carlo simulations build maps predicted connectivity based...
The claustrum is thought to be one of the most highly interconnected forebrain structures but its organizing principles have yet fully explored at level single neurons. Here, we investigated identity, connectivity, and activity identified neurons understand how structure's unique convergence input divergence output support binding information streams. We found that in communicate with each other across efferent projection-defined modules which were differentially innervated by sensory...
Recent advances in experimental stimulation methods have raised the following important computational question: how can we choose a stimulus that will drive neuron to output target spike train with optimal precision, given physiological constraints? Here adopt an approach based on models describe stimulating agent (such as injected electrical current or laser light interacting caged neurotransmitters photosensitive ion channels) affects spiking activity of neurons. Based these models, solve...
Our understanding of the link between neural activity and perception remains incomplete. Microstimulation optogenetic experiments have shown that manipulating cortical can influence sensory-guided behaviour or elicit artificial percepts. And yet, some perceptual tasks still be solved when sensory cortex is silenced removed, suggesting may not always essential. Reconciling these findings, providing a quantitative framework linking behaviour, requires knowledge identity cells being activated...