A5037720828- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Neuroendocrine regulation and behavior
- Memory and Neural Mechanisms
- Molecular Communication and Nanonetworks
- Cellular transport and secretion
- Advanced Fluorescence Microscopy Techniques
- Lipid Membrane Structure and Behavior
- Synthesis of Tetrazole Derivatives
- Retinal Development and Disorders
- Stress Responses and Cortisol
- Protein Kinase Regulation and GTPase Signaling
- Plant and Biological Electrophysiology Studies
- Circadian rhythm and melatonin
- Light effects on plants
- Cell death mechanisms and regulation
- Photosynthetic Processes and Mechanisms
- Electrochemical Analysis and Applications
- Sleep and Wakefulness Research
- Olfactory and Sensory Function Studies
- Calcium signaling and nucleotide metabolism
- Receptor Mechanisms and Signaling
- Ion channel regulation and function
Weizmann Institute of Science
2015-2024
Stanford University
2008-2017
Howard Hughes Medical Institute
2011-2013
Bioengineering Center
2012
Tel Aviv University
2004-2010
Hebrew University of Jerusalem
2005
Saarland University
2004
Abstract Optogenetic silencing allows time-resolved functional interrogation of defined neuronal populations. However, the limitations inhibitory optogenetic tools impose stringent constraints on experimental paradigms. The high light power requirement light-driven ion pumps and their effects intracellular homeostasis pose unique challenges, particularly in experiments that demand inhibition a widespread population vivo. Guillardia theta anion-conducting channelrhodopsins (GtACRs) are...
In vivo experimental evidence indicates that acute neuronal activation increases Aβ release from presynaptic terminals, whereas long-term effects of chronic synaptic on pathology remain unclear. To address this issue, we adopted optogenetics and transduced stabilized step-function opsin, a channelrhodopsin engineered to elicit long-lasting hyperexcitability, into the hippocampal perforant pathway APP transgenic mice. microdialysis revealed ∼24% increase in interstitial fluid Aβ42 levels...
A defining feature of sleep is reduced responsiveness to external stimuli, but the mechanisms mediating sensory-evoked arousal remain unclear. We hypothesized that locus coeruleus (LC) norepinephrine (NE) activity during mediates unresponsiveness, and its action promotes awakenings. tested this using electrophysiological, behavioral, pharmacological, optogenetic techniques alongside auditory stimulation in freely behaving rats. found systemic reduction NE signaling lowered probability...