A5004459696- Photoreceptor and optogenetics research
- Neural dynamics and brain function
- Neuroscience and Neural Engineering
- Neuroscience and Neuropharmacology Research
- Molecular Communication and Nanonetworks
- Tryptophan and brain disorders
- Stress Responses and Cortisol
- Neuroendocrine regulation and behavior
- Advanced Fluorescence Microscopy Techniques
Weizmann Institute of Science
2016-2023
Abstract Optogenetic silencing allows time-resolved functional interrogation of defined neuronal populations. However, the limitations inhibitory optogenetic tools impose stringent constraints on experimental paradigms. The high light power requirement light-driven ion pumps and their effects intracellular homeostasis pose unique challenges, particularly in experiments that demand inhibition a widespread population vivo. Guillardia theta anion-conducting channelrhodopsins (GtACRs) are...
Abstract The medial prefrontal cortex (mPFC) mediates a variety of complex cognitive functions via its vast and diverse connections with cortical subcortical structures. Understanding the patterns synaptic connectivity that comprise mPFC local network is crucial for deciphering how this circuit processes information relays it to downstream To elucidate organization mPFC, we developed high-throughput optogenetic method mapping large-scale functional in acute brain slices. We show male mice,...
The bed nucleus of the stria terminalis (BNST) is critical in mediating states anxiety, and its dysfunction has been linked to stress-related mental disease. Although anxiety-related role distinct subregions anterior BNST was recently reported, little known about contribution posterior (pBNST) behavioral neuroendocrine responses stress. Previously, we observed abnormal expression corticotropin-releasing factor receptor type 2 (CRFR2) be associated with post-traumatic stress disorder...
All-optical methods for imaging and manipulating brain networks with high spatial resolution are fundamental to study how neuronal ensembles drive behavior. Stimulation of using two-photon holographic techniques requires high-sensitivity actuators avoid photodamage heating. Moreover, two-photon-excitable opsins should be insensitive light at wavelengths used imaging. To achieve this goal, we developed a novel soma-targeted variant the large-conductance blue-light-sensitive opsin CoChR...
Abstract Optogenetic silencing allows time-resolved functional interrogation of defined neuronal populations. However, the limitations inhibitory optogenetic tools impose stringent constraints on experimental paradigms. The high light power requirement light-driven ion pumps and their effects intracellular homeostasis pose unique challenges, particularly in experiments that demand inhibition a widespread population vivo. Guillardia theta anion-conducting channelrhodopsins (GtACRs) are...
Abstract The medial prefrontal cortex (mPFC) mediates a variety of complex cognitive functions via its vast and diverse connections with cortical subcortical structures. Understanding the patterns synaptic connectivity that comprise mPFC local network is crucial for deciphering how this circuit processes information relays it to downstream To elucidate organization mPFC, we developed high-throughput optogenetic method mapping large-scale functional connectivity. We show neurons project...
We performed two-photon holographic stimulation of blue light-sensitive excitatory opsins using high- and low-repetition rate lasers we demonstrate high-efficiency neural with few mW average power per cell in the intact mouse cortex.