A5020884076- Photoreceptor and optogenetics research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Optical Imaging and Spectroscopy Techniques
- Optical Coherence Tomography Applications
- Advanced Fluorescence Microscopy Techniques
- Photonic and Optical Devices
- Molecular Communication and Nanonetworks
- Medical Imaging Techniques and Applications
- Photorefractive and Nonlinear Optics
- Mechanical and Optical Resonators
- Electrical and Bioimpedance Tomography
- Traumatic Brain Injury and Neurovascular Disturbances
- Photoacoustic and Ultrasonic Imaging
- Advanced Memory and Neural Computing
- Advanced X-ray and CT Imaging
- Glaucoma and retinal disorders
- Advanced Fiber Laser Technologies
- Spaceflight effects on biology
- Advanced Optical Imaging Technologies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Quantum Chemical Studies
- Optical and Acousto-Optic Technologies
- Neural Networks and Applications
- Quantum optics and atomic interactions
Florida Atlantic University
2021-2024
University of Wisconsin–Milwaukee
2011-2020
Marquette University
2014
Stanford University
2009-2011
University of Pennsylvania
2006-2007
California University of Pennsylvania
2007
Philadelphia University
2007
University of Pennsylvania Health System
2006
Abstract Neural micro-electrode arrays that are transparent over a broad wavelength spectrum from ultraviolet to infrared could allow for simultaneous electrophysiology and optical imaging, as well optogenetic modulation of the underlying brain tissue. The long-term biocompatibility reliability neural micro-electrodes also require their mechanical flexibility compliance with soft tissues. Here we present graphene-based, carbon-layered electrode array (CLEAR) device, which can be implanted on...
Electrical stimulation using implantable electrodes is widely used to treat various neuronal disorders such as Parkinson's disease and epilepsy a research tool in neuroscience studies. However, date, devices that help better understand the mechanisms of electrical neural tissues have been limited opaque electrodes. Imaging spatiotemporal responses with minimal artifact could allow for studies are impossible existing Here, we demonstrate brain simultaneous optical monitoring underlying...
Predicting the distribution of light inside any turbid media, such as biological tissue, requires detailed information about optical properties medium, including absorption and scattering coefficients anisotropy factor. Particularly, in biophotonic applications where photons directly interact with this translates to system design optimization, precision delivery, minimization unintended consequences, phototoxicity or photobleaching. In recent years, optogenetics has opened up a new area deep...
There is great interest in designing implantable neural electrode arrays that maximize function while minimizing tissue effects and damage. Although it has been shown substrate geometry plays a key role the response to intracortically implanted, penetrating interfaces, there minimal investigation into effect of footprint on surface arrays. This study investigates micro-electrocorticography (micro-ECoG) device longitudinal response.The meningeal two micro-ECoG devices with differing...
Spatial localization of neural activity from within the brain with electrocorticography (ECoG) and electroencephalography remains a challenge in clinical research settings, while microfabricated ECoG (micro-ECoG) array technology continues to improve, complementary methods simultaneously modulate cortical recording are needed.We developed interface utilizing optogenetics, cranial windowing, micro-ECoG arrays fabricated on transparent polymer. This approach enabled us directly at known...
Optimizing light delivery for optogenetics is critical in order to accurately stimulate the neurons of interest while reducing nonspecific effects such as tissue heating or photodamage. Light distribution typically predicted using assumption homogeneity, which oversimplifies transport heterogeneous brain. Here, we present an open-source 3D simulation platform, OptogenSIM, eliminates this assumption. This platform integrates a voxel-based Monte Carlo model, generic optical property models...
This paper presents a new approach for implementation of closed-loop brain-machine interface algorithms by combining optogenetic neural stimulation with electrocorticography and fluorescence microscopy. We used generation microfabricated (micro-ECoG) devices in which electrode arrays are embedded within an optically transparent biocompatible substrate that provides optical access to the brain tissue during electrophysiology recording. An setup was designed capable projecting arbitrary...
We have developed a fiber-optic-based probe for precise delivery of stimulation/excitation light pulses and detection faint fluorescence signals applications in neuroscience optogenetics. In this design, thin multimode fiber serves as the head to be inserted into brain. This is used deliver region interest guide sample emission signal back detectors. The major tradeoff design such system decrease size intensity input minimize physical damage avoid photobleaching/phototoxicity but keep...
Digital optical phase conjugation (DOPC) has proven to be a promising technique in deep tissue fluorescence imaging. Nonetheless, DOPC setups require precise alignment of all components accurately read the wavefront scattered light turbid medium and playback conjugated beam toward sample. Minor misalignments possible imperfections arrangement or structure significantly reduce performance method. In this paper, calibration procedure based on orthogonal rectangular polynomials is introduced...
In this article, spectral domain optical coherence tomography is used to measure the hemodynamic response induced by optogenetic stimulation in somatosensory cortex of transgenic mice. By analyzing 3-D angiograms and Doppler measurements produced tomography, we observed significant increase blood flow as a result increased vessel diameter velocity following cortical neurons. Such distinct responses were not control experiments where brain wild-type mice exposed same light pulses.
In this Letter, the impact of blood vessels on light distribution during photostimulation cortical tissue in small rodents is investigated. Brain optical properties were extracted using a double-integrating sphere setup, and coherence tomography was used to image capillaries generate three-dimensional angiogram cortex. By combining these two datasets, complete volumetric structure developed linked Monte Carlo code which simulates propagation inhomogeneous illustrates effect penetration depth...
The hemodynamic and metabolic response of the cortex depends spatially temporally on activity multiple cell types. Optogenetics enables specific types to be modulated with high temporal precision is therefore an emerging method for studying neurovascular neurometabolic coupling. Going beyond investigations, we developed a microprojection system apply spatial photostimulus patterns in vivo. We monitored vascular fluorescence signals after photostimulation Thy1-channelrhodopsin-2 mice....
Combining an optical coherence tomography (OCT) scanner with other techniques such as optogenetic neurostimulation or fluorescence imaging requires integrating auxiliary components into the path of setup. Due to short scanning distance most OCT objectives, adding scan and tube lenses in device is essential open space between back-focal-plane objective center mass mirrors galvanometer. The effect on focal spot size using off-the-shelf are theoretically explored for three different designs...
Tomography is widely used in medical imaging or industrial non-destructive testing applications. One costly and time consuming operation any form of tomography the process data acquisition where a large number measurements are made collected for image reconstruction. Data can slow down to point that scanner cannot catch up with speed changes medium under test. By optimizing information content each measurement, we reduce needed achieve target precision. Development algorithms optimize main...
In this paper, a new model seeking to emulate the way visual cortex processes information and interacts with subcortical areas produce higher level brain functions is described. We developed macroscopic approach that incorporates salient attributes of based on combining tools nonlinear dynamics, theory, known organizational anatomical features cortex. Justifications for demonstration its effectiveness are presented. also demonstrate certain capabilities in producing efficient sparse...
In the past decades, spectral-domain optical coherence tomography (SD-OCT) has transformed into a widely popular imaging technology which is used in many research and clinical applications. Despite such fast growth field, not been readily accessible to laboratories either due cost or inflexibility of commercially available systems lack essential knowledge field optics develop custom-made scanners that suit specific This paper aims provide detailed discussion on design development process...
We introduce an engineering approach to study spatiotemporal correlations between vasodynamics and the nearby neural activity in open-loop closed-loop paradigms.We integrated optogenetic technology with optical coherence tomography apply patterns of neurostimulation cortex transgenic mice measure blood flow-rate, velocity, diameter changes selected middle cerebral artery branches.The characteristics vessel responses localized light pulses were measured. It was observed that location...
A thin film of electron-trapping material (ETM), when combined with suitable optical bistability, is considered as a medium for implementation bioinspired neural nets. The mechanism ETM under blue light and near-infrared exposure has the inherent ability at level to mimic crucial components stylized Hodgkin-Huxley model biological neurons. Combining this unique property high-resolution capability ETM, dense network neurons can be realized in infrared stimulable storage phosphor. When...
Exploiting salient features in the photodynamics of specific types light sensitive materials, a new approach is presented for realization parallel nonlinear operations with optics. We briefly review quantum structure and mathematical models offered two multiwavelength doped crystals lithium niobate thick layers bacteriorhodopsin. Next, special mode these dynamics each material investigated graphical design procedure to produce highly optical responses that can be dynamically reshaped via...
One of the main issues related with wind energy systems is unpredictable and fluctuating nature extracted power. The combinations different types storage control strategies have been proposed in literature order to improve power quality coming from turbines. In current paper, a novel neural network based system for entire farm distributed system. Storage integrated every turbine (WT) reduces total output fluctuations by using available among turbines cooperative manner. For verification...
We present our effort in implementing a fluorescence laminar optical tomography scanner which is specifically designed for noninvasive three-dimensional imaging of proteins the brains small rodents. A laser beam, after passing through cylindrical lens, scans brain tissue from surface while emission signal captured by epi-fluorescence optics and recorded using an electron multiplication CCD sensor. Image reconstruction algorithms are developed based on Monte Carlo simulation to model...