A5088165194- Neurobiology and Insect Physiology Research
- Photoreceptor and optogenetics research
- Receptor Mechanisms and Signaling
- Circadian rhythm and melatonin
- Animal Behavior and Reproduction
- Retinal Development and Disorders
- Neural dynamics and brain function
- Sleep and Wakefulness Research
- Molecular Communication and Nanonetworks
- Insect and Arachnid Ecology and Behavior
- Neuroscience and Neural Engineering
University Medical Center Hamburg-Eppendorf
2020-2023
Universität Hamburg
2020-2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2023
University of Bonn
2021-2023
Abstract Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control allowing both excitation inhibition the same neurons a single experiment is desired. This requires low spectral overlap between opsin, matched photocurrent amplitudes fixed expression ratio. Moreover, independent activation two distinct populations with different optogenetic...
Neuromodulatory signaling via G protein-coupled receptors (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. The recent development of optogenetic tools to induce protein-mediated provides the promise acute cell type-specific manipulation neuromodulatory signals. However, designing deploying optogenetically functionalized GPCRs (optoXRs) with accurate specificity activity mimic endogenous vivo remains challenging. Here we optimize design optoXRs by...
Abstract Animals display a plethora of escape behaviors when faced with environmental threats. Selection the appropriate response by underlying neuronal network is key to maximize chances survival. We uncovered somatosensory in Drosophila larvae that encodes two through input-specific neuropeptide action. Sensory neurons required for avoidance noxious light and harsh touch, each converge on discrete domains same neuromodulatory hub neurons. These gate touch responses via short Neuropeptide...
Abstract Information is carried between brain regions through neurotransmitter release from axonal presynaptic terminals. Understanding the functional roles of defined neuronal projection pathways in cognitive and behavioral processes requires temporally precise manipulation their activity vivo . However, existing optogenetic tools have low efficacy off-target effects when applied to terminals, while chemogenetic are difficult control space time. Here, we show that a targeting-enhanced...
Abstract Neuromodulatory signaling via G protein-coupled receptor (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. Recent efforts have led to the development of optogenetic tools induce protein-mediated signaling, with promise acute cell type-specific manipulation neuromodulatory signals. However, designing deploying optogenetically functionalized GPCRs (optoXRs) accurate specificity activity mimic endogenous vivo remains challenging. Here we optimized...
Two-choice assays allow assessing of different behaviors including light avoidance in Drosophila larvae. Typically, the readout is limited to a preference index at specific end point. We provide detailed protocol set up and map temporal distribution larvae based on analysis larval intensities. describe assay setup implementation scripts for analysis, which can be easily adapted other two-choice model organisms. For complete details use execution this protocol, please refer Imambocus et al. (2022).