A5103261061- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Mass Spectrometry Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
- Neuropeptides and Animal Physiology
- Neurotransmitter Receptor Influence on Behavior
- Drug Transport and Resistance Mechanisms
- Molecular Communication and Nanonetworks
- Amino Acid Enzymes and Metabolism
- RNA modifications and cancer
- Trace Elements in Health
- Pain Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Ion channel regulation and function
- Olfactory and Sensory Function Studies
- PI3K/AKT/mTOR signaling in cancer
- Biochemical Analysis and Sensing Techniques
- bioluminescence and chemiluminescence research
- Neurobiology and Insect Physiology Research
- Enzyme Structure and Function
- Neuroscience and Neural Engineering
- Cancer, Hypoxia, and Metabolism
- Antioxidant Activity and Oxidative Stress
The University of Tokyo
2013-2025
Japan Science and Technology Agency
2021-2025
Tokyo University of the Arts
2022-2025
Shizuoka University
2009-2023
Nagoya City University
2007-2022
Stanford University
2014-2022
Mizuho (Japan)
2016-2022
Futaba (Japan)
2014-2020
Shionogi (Japan)
2014-2020
International University of Health and Welfare
2013-2018
Perceptual experiences may arise from neuronal activity patterns in mammalian neocortex. We probed mouse neocortex during visual discrimination using a red-shifted channelrhodopsin (ChRmine, discovered through structure-guided genome mining) alongside multiplexed multiphoton-holography (MultiSLM), achieving control of individually specified neurons spanning large cortical volumes with millisecond precision. Stimulating critical number stimulus-orientation-selective drove widespread...
ChRmine, a recently discovered pump-like cation-conducting channelrhodopsin, exhibits puzzling properties (large photocurrents, red-shifted spectrum, and extreme light sensitivity) that have created new opportunities in optogenetics. ChRmine its homologs function as ion channels but, by primary sequence, more closely resemble pump rhodopsins; mechanisms for passive channel conduction this family remained mysterious. Here, we present the 2.0 Å resolution cryo-EM structure of revealing...
The aim of the present study was to examine DNA methylation and histone modification changes in hepatocellular carcinomas (HCC).DNA P16, RASSF1a, progesterone receptor (PGR) estrogen alpha (ERalpha) promoters determined by quantitative bisulfite-pyrosequencing technique HCC patients. Histone H3-lysine (K) 4, H3-K9 H3-K27 modifications all these four genes were examined chromatin immunoprecipitation (ChIP) assay cell lines. Expression two methyltransferases (DNMT1 DNMT3b) three (SUV39H1, G9a...
Proton-dependent oligopeptide transporters (POTs) are major facilitator superfamily (MFS) proteins that mediate the uptake of peptides and peptide-like molecules, using inwardly directed H(+) gradient across membrane. The human POT family transporter peptide 1 is present in brush border membrane small intestine involved nutrient drug molecules such as β-lactam antibiotics. Although previous studies have provided insight into overall structure transporters, question how transport coupled to...
Abstract In vertebrates, the iron exporter ferroportin releases Fe 2+ from cells into plasma, thereby maintaining homeostasis. The transport activity of is suppressed by peptide hormone hepcidin, which exhibits upregulated expression in chronic inflammation, causing iron-restrictive anaemia. However, due to lack structural information about ferroportin, mechanisms its and hepcidin-mediated regulation remain largely elusive. Here we report crystal structures a putative bacterial homologue...
Abstract Microbial opsins with a bound chromophore function as photosensitive ion transporters and have been employed in optogenetics for the optical control of neuronal activity. Molecular engineering has utilized to create colour variants functional augmentation tools, but was limited by complexity protein–chromophore interactions. Here we report development blue-shifted rational design at atomic resolution, achieved through accurate hybrid molecular simulations, electrophysiology X-ray...
KCR channelrhodopsins (K
Objective— Apelin is an endogenous ligand for the G protein-coupled receptor, APJ, and participates in multiple physiological processes. To identify roles of apelin, we investigated phenotype apelin-deficient (apelin-KO) mice. Methods Results— Apelin-KO mice showed impaired retinal vascularization ocular development, which were analyzed by histology, immunohistochemistry, real-time polymerase chain reaction, mouse corneal micropocket assay. significantly early postnatal period. Retinal...
Endogenous parathyroid hormone (PTH) and PTH-related peptide (PTHrP) bind to the receptor 1 (PTH1R) activate stimulatory G-protein (Gs) signaling pathway. Intriguingly, two ligands have distinct physiological properties: PTH evokes prolonged Gs activation, whereas PTHrP transient activation with reduced bone-resorption effects. The molecular actions are ascribed differences in ligand recognition dissociation kinetics. Here, we report cryoelectron microscopic structures of six forms human...
Human cytomegalovirus (HCMV) encodes G protein–coupled receptors (GPCRs) US28 and US27 , which facilitate viral pathogenesis through engagement of host proteins. Here we report cryo–electron microscopy structures forming nonproductive productive complexes with Gi Gq, respectively, exhibiting unusual features functional implications. The “orphan” GPCR lacks a ligand-binding pocket has captured guanosine diphosphate–bound inactive tenuous interaction. docking modes CX3CL1-US28 to favor...
A phylogenetic analysis, using the open reading frame I sequence of 93 TT viruses (TTV) obtained from various geographical areas, indicated that virus could be classified into six different genotypes including three hitherto unreported genotypes. The high reliability clusters was confirmed by bootstrap analysis. On basis these data, a new simple genotyping assay based on restriction fragment length polymorphism TTV developed. Using enzymes NdeI and PstI, followed cleavage with NlaIII or...
Channelrhodopsin (ChR) functions as a light-gated ion channel in Chlamydomonas reinhardtii. Passive transport of cations by ChR is fundamentally different from the active light-driven pumps such archaerhodopsin, bacteriorhodopsin, and halorhodopsin. These microbial rhodopsins are important tools for optogenetics, where used to activate neurons light, while neural silencing. Ion-transport these strongly depend on specific hydrogen-bonding networks containing water near retinal chromophore. In...