A5068104880- Receptor Mechanisms and Signaling
- Neuropeptides and Animal Physiology
- Mass Spectrometry Techniques and Applications
- Lipid Membrane Structure and Behavior
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Ion channel regulation and function
- Photoreceptor and optogenetics research
- Diabetes Treatment and Management
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Chemokine receptors and signaling
- Chemical Synthesis and Analysis
- Ion Channels and Receptors
- Sphingolipid Metabolism and Signaling
- Glycosylation and Glycoproteins Research
- Heat shock proteins research
- Viral Infections and Vectors
- Advanced MEMS and NEMS Technologies
- Immunotherapy and Immune Responses
- Plant tissue culture and regeneration
- Cardiac electrophysiology and arrhythmias
- Advanced Electron Microscopy Techniques and Applications
- Retinal Development and Disorders
- Erythrocyte Function and Pathophysiology
Chinese University of Hong Kong, Shenzhen
2020-2025
Chinese University of Hong Kong
2022
Stanford University
2018-2020
State Key Laboratory of Oral Diseases
2020
Sichuan University
2020
Peking University
2010-2020
University of Michigan
2017
Ann Arbor Center for Independent Living
2017
New York State Department of Health
2015-2016
Stomatology Hospital
2016
Abstract Single-particle cryo-electron microscopy (cryo-EM) has recently enabled high-resolution structure determination of numerous biological macromolecular complexes. Despite this progress, the application cryo-EM to G protein coupled receptors (GPCRs) in complex with heterotrimeric proteins remains challenging, owning both relative small size and limited stability these assemblies. Here we describe development antibody fragments that bind stabilize GPCR-G complexes for cryo-EM. One...
Family B heterotrimeric guanine nucleotide-binding protein (G protein)-coupled receptors (GPCRs) play important roles in carbohydrate metabolism. Recent structures of family GPCR-Gs complexes reveal a disruption the α-helix transmembrane segment 6 (TM6) not observed A GPCRs. To investigate functional impact this structural difference, we compared structure and function glucagon receptor (GCGR; B) with β2 adrenergic (β2AR; A). We determined GCGR-Gs complex by means cryo-electron microscopy at...
As a critical sphingolipid metabolite, sphingosine-1-phosphate (S1P) plays an essential role in immune and vascular systems. There are five S1P receptors, designated as S1PR1 to S1PR5, encoded the human genome, their activities governed by endogenous S1P, lipid-like mimics, or nonlipid-like therapeutic molecules. Among S1PRs, stands out due its nonredundant functions, such egress of T B cells from thymus secondary lymphoid tissues, making it potential target. However, structural basis...
The chemotaxis of CD4
Flaviviruses encode a conserved, membrane-associated nonstructural protein 1 (NS1) with replication and immune evasion functions. The current knowledge of secreted NS1 (sNS1) oligomers is based on several low-resolution structures, thus hindering the development drugs vaccines against flaviviruses. Here, we revealed that recombinant sNS1 from flaviviruses exists in dynamic equilibrium dimer-tetramer-hexamer states. Two DENV4 hexameric structures tetrameric multiple were solved at atomic...
Abstract The formyl peptide receptor 1 (FPR1) is primarily responsible for detection of short peptides bearing N-formylated methionine (fMet) that are characteristic protein synthesis in bacteria and mitochondria. As a result, FPR1 critical to phagocyte migration activation bacterial infection, tissue injury inflammation. How distinguishes between non-formyl remains elusive. Here we report cryo-EM structures human FPR1-Gi complex bound S. aureus -derived fMet-Ile-Phe-Leu (fMIFL) E. coli...
Abstract Small heat shock proteins (sHSPs) are molecular chaperones ubiquitously present in all forms of life, but their function mechanisms remain controversial. Here we show by cryo-electron microscopy and single particle 3D reconstruction that, at the low temperatures (4–25°C), CeHSP17 (a sHSP from Caenorhabditis elegans ) exists as a 24-subunit spherical oligomer with tetrahedral symmetry. Our studies demonstrate that large sheet-like super-molecular assemblies (SMAs) high (45–60°C) such...
Human jaw bone marrow mesenchymal stem cells (h-JBMMSCs) are multipotent progenitor with osteogenic differentiation potential. The relationship between adiponectin (APN) and the metabolism of h-JBMMSCs has not been fully elucidated, underlying mechanism remains unclear. aim study was to investigate effect APN on h-JBMMSC metabolism. were obtained from primary culture human bones treated or without (1 µg/mL). Osteogenesis-related gene expression evaluated by real-time polymerase chain...
Calcitonin gene-related peptide (CGRP) plays a crucial role in the modulation of orofacial pain, and we hypothesized that CGRP mediated neuron-glia crosstalk pain. The objective this study was to elucidate mechanisms whereby trigeminal modulating Orofacial pain elicited by ligating closed-coil springs between incisors molars. Trigeminal neurons satellite glial cells (SGCs) were cultured for mechanistic exploration. Gene protein expression determined through immunostaining, polymerase chain...
Mas-related G-protein-coupled receptors X1-X4 (MRGPRX1-X4) are 4 primate-specific that recently reported to be responsible for many biological processes, including itch sensation, pain transmission, and inflammatory reactions. MRGPRX1 is the first identified human MRGPR, its expression restricted primary sensory neurons. Due dual roles in signaling pathways, has been regarded as a promising target remission inhibition. Here, we cryo-electron microscopy (cryo-EM) structure of Gq-coupled...