A5077151053- Advanced biosensing and bioanalysis techniques
- CRISPR and Genetic Engineering
- DNA and Nucleic Acid Chemistry
- RNA Interference and Gene Delivery
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Salmonella and Campylobacter epidemiology
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Biochemical and Molecular Research
- DNA Repair Mechanisms
- Bacterial Genetics and Biotechnology
- Protein purification and stability
- Monoclonal and Polyclonal Antibodies Research
- Forensic and Genetic Research
- Blood groups and transfusion
- Erythrocyte Function and Pathophysiology
- HIV/AIDS drug development and treatment
- RNA Research and Splicing
- Escherichia coli research studies
- Photoreceptor and optogenetics research
- Cystic Fibrosis Research Advances
- Salivary Gland Disorders and Functions
- Enzyme Production and Characterization
- Neuroscience and Neuropharmacology Research
The University of Tokyo
1980-2023
International University of Health and Welfare
2015
Gunma University
1998-2007
Jichi Medical University
1987-2005
University of Fukui
1988-2004
University of Occupational and Environmental Health Japan
2004
Tohoku Medical and Pharmaceutical University
2004
Gunma Prefectural Cardiovascular Center
2004
National Research Institute of Police Science
1975-1983
Chiba University
1981-1982
ChRmine, a recently discovered pump-like cation-conducting channelrhodopsin, exhibits puzzling properties (large photocurrents, red-shifted spectrum, and extreme light sensitivity) that have created new opportunities in optogenetics. ChRmine its homologs function as ion channels but, by primary sequence, more closely resemble pump rhodopsins; mechanisms for passive channel conduction this family remained mysterious. Here, we present the 2.0 Å resolution cryo-EM structure of revealing...
In the single radial enzyme-diffusion (SRED) method for assay of deoxyribonuclease I, a precisely measured volume enzyme solution is dispensed into circular well in an agarose gel layer which DNA and ethidium bromide are uniformly distributed. A dark zone formed as diffuses from radially digests substrate DNA. The diameter circle hydrolyzed increases size with time correlates linearly amount applied to well. Thus, SRED can be used quantitation I limit detection 2 x 10(-6) unit. This...
KCR channelrhodopsins (K
A deoxyribonuclease I was purified from the urine of a 46-year-old male (a single individual) by using series column chromatographies to homogeneous state as judged sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The enzyme found be glycoprotein, containing 1 fucose, 7 galactose, 10 mannose, 6 glucosamine, and 2 sialic acid residues per molecule. N-terminal amino sequence up 27th residue similar that pancreatic bovine other species. catalytic properties derived individual closely...
We have observed three calmodulin mRNA species in rat tissues. In order to know from how many expressed genes they are derived, we investigated the genomic organization of genome. From a brain cDNA library, obtained two kinds cDNAs (pRCM1 and pRCM3) encoding authentic calmodulin. DNA sequence analysis these clones revealed substitutions nucleotides at 73 positions 450 coding region, although amino acid sequences calmodulins exactly same. 5' 3' noncoding regions quite different between cDNAs....
A rapid amplification of cDNA ends method, using degenerate oligonucleotides based upon the N-terminal amino acid sequence human hepatic deoxyribonuclease II (DNase II), allowed a novel encoding DNase to be constructed from thyroid gland RNA. The composite nucleotide (1593 bases) included an open reading frame 1080 bases, which encoded single polypeptide 360 acids (signal peptide, 16; propeptide, 91; mature protein, 253). Although showed no significant homology other mammalian proteins, its...
Summary The objectives of this study were to elucidate the structural organization gene for human deoxyribonuclease I (DNase I) and identify mutation site underlying its classical genetic polymorphism. In order determine gene, we utilized a combination direct polymerase chain reaction (PCR)‐amplification genomic DNA isolation overlapping clones from cosmid library. Restriction endonuclease mapping, Southern blotting sequencing showed that DNase was approximately 3·2 kilobases long, it...
Background— The delayed release of serum cardiac markers such as creatine kinase isoenzyme MB and equivocal early electrocardiographic changes have hampered a diagnosis acute myocardial infarction (AMI) in the phase after its onset. Therefore, reliable biochemical marker for AMI very is desirable. Methods Results— Serum samples were collected from patients with AMI, unstable angina pectoris, stable other diseases. Levels deoxyribonuclease I (DNase I) activity determined. An abrupt elevation...
Polymorphism of α2-HS-glycoprotein (AHSG) was revealed in human urine by isoelectric focusing and immunoblotting on polyacrylamide gels. More than 200 samples were examined this manner correct AHSG typing the achieved, comparison with results direct grouping for plasma. Three phenotypes, 1,2–1 2, observed found to be determined two common alleles, AHSG*! AHSG*2. The frequencies AHSG*l AHSG*2 calculated a Japanese population 0.7637 0.2363, respectively.
DNase I from rabbit urine was purified approx. 3600-fold to apparent homogeneity with a 41% yield by affinity chromatography utilizing DNA–cellulose; the purity of final preparation assessed SDS/PAGE, lack contamination other nucleases and production monospecific antibody against enzyme. Although proteochemical enzymological properties enzyme resembled those mammalian DNases I, enzymic activity less efficiently inhibited monomeric actin than that human probably due substitution an amino acid...
Abstract Mouse urinary deoxyribonuclease I (DNase I) resembles rat and human DNase Is in terms of its proteochemical enzymological properties. Furthermore, mouse was demonstrated to be immunologically closer the than enzyme. A 1176 bp full length cDNA encoding constructed from RNA obtained kidney parotid glands. The amino acid sequence up 45th residue N‐terminal mature enzyme identical that deduced sequence. This distributed most densely glands standpoint both activity gene transcript levels.
M-LP (Mpv17-like protein) has been identified as a new protein that high sequence homology with Mpv17 protein, peroxisomal membrane involved in the development of early onset glomerulosclerosis. In this study, we verified localization by performing dual-color confocal analysis COS-7 cells cotransfected green fluorescent protein-tagged and DsRED2-PTS1, red marker. To characterize targeting signal, examined intracellular localizations several deletion mutants demonstrated that, three...
We have recently reported that serum deoxyribonuclease I (DNase I) activity, which may be involved in apoptosis, increases abruptly the early phase of acute myocardial infarction (MI) [Kawai Y, Yoshida M, Arakawa K, Kumamoto T, Morikawa N, Masamura Tada H, Ito S, Hoshizaki Oshima Taniguchi Terasawa Miyamori I, Kishi Yasuda T. Diagnostic use activity as a novel early-phase marker infarction. Circulation 2004;109:2398-2400]. Death vascular smooth muscle cells, part because is postulated to...