A5015588106- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Receptor Mechanisms and Signaling
- Molecular Communication and Nanonetworks
- Memory and Neural Mechanisms
- Neuroendocrine regulation and behavior
- Neurotransmitter Receptor Influence on Behavior
- Circadian rhythm and melatonin
- Sleep and Wakefulness Research
- Neurobiology and Insect Physiology Research
- Light effects on plants
- CRISPR and Genetic Engineering
- Nicotinic Acetylcholine Receptors Study
- Photosynthetic Processes and Mechanisms
- Regulation of Appetite and Obesity
- Gene Regulatory Network Analysis
- Neuroscience of respiration and sleep
- Genetics, Aging, and Longevity in Model Organisms
- Plant and Biological Electrophysiology Studies
- Single-cell and spatial transcriptomics
- Advanced Fluorescence Microscopy Techniques
- Pluripotent Stem Cells Research
- Transcranial Magnetic Stimulation Studies
The University of Texas at Austin
2022-2025
Stanford University
2015-2024
Stanford Medicine
2022
Howard Hughes Medical Institute
2011-2021
Bioengineering Center
2009-2014
Society for Neuroscience
2014
Harvard Stem Cell Institute
2008
Massachusetts General Hospital
2008
Stowers Institute for Medical Research
2007
Water deprivation produces a drive to seek and consume water. How neural activity creates this motivation remains poorly understood. We used activity-dependent genetic labeling characterize neurons activated by water in the hypothalamic median preoptic nucleus (MnPO). Single-cell transcriptional profiling revealed that dehydration-activated MnPO consist of single excitatory cell type. After optogenetic activation these neurons, mice drank performed an operant lever-pressing task for reward...
Parkinson's disease (PD) is an incurable age-related neurodegenerative disorder affecting both the central and peripheral nervous systems. Although common, etiology of PD remains poorly understood. Genetic studies infer that results from a complex interaction between genetics environment there growing evidence may represent constellation diseases with overlapping yet distinct underlying mechanisms. Novel clinical approaches will require better understanding mechanisms at work within...
Alterations in the balance between neuronal excitation and inhibition (E:I balance) have been implicated neural circuit activity-based processes that contribute to autism phenotypes. We investigated whether acutely reducing E:I mouse brain could correct deficits social behavior. used mice lacking CNTNAP2 gene, which has autism, achieved a temporally precise reduction medial prefrontal cortex (mPFC) either by optogenetically increasing excitability of inhibitory parvalbumin (PV) neurons or...
In vivo experimental evidence indicates that acute neuronal activation increases Aβ release from presynaptic terminals, whereas long-term effects of chronic synaptic on pathology remain unclear. To address this issue, we adopted optogenetics and transduced stabilized step-function opsin, a channelrhodopsin engineered to elicit long-lasting hyperexcitability, into the hippocampal perforant pathway APP transgenic mice. microdialysis revealed ∼24% increase in interstitial fluid Aβ42 levels...
Highlights•nRT PV, but not SOM, neurons exhibit intrinsic rhythmogenic properties•nRT PV and SOM segregate into separate thalamocortical circuits•nRT differentially modulate oscillations in somatosensory cortex•nRT cells are preferentially engaged behavior seizuresSummaryIntegrative brain functions depend on widely distributed, rhythmically coordinated computations. Through its long-ranging connections with cortex most senses, the thalamus orchestrates flow of cognitive sensory information....
Significance Stroke is the leading cause of disability in United States and has very limited treatment options. Brain stimulation techniques that promote recovery after stroke are a promising area research; however, current nonspecifically activate/inhibit target area, which not only leads to undesired side effects but also makes it difficult understand cell types mechanisms drive recovery. We used optogenetic technique specifically stimulate neurons demonstrate selective neuronal...
The structural and functional complexity of multicellular biological systems, such as the brain, are beyond reach human design or assembly capabilities. Cells in living organisms may be recruited to construct synthetic materials structures if treated anatomically defined compartments for specific chemistry, harnessing biology complex structures. By integrating engineered-enzyme targeting polymer we genetically instructed neurons guide chemical synthesis electrically (conductive insulating)...
Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry neuron-subtype specificity. However, due limited tissue penetration light, invasive craniotomy and intracranial implantation tethered optical fibers are usually required in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local sources brain when triggered by...
Encoding and predicting aversive events are critical functions of circuits that support survival emotional well-being. Maladaptive circuit changes in valence processing can underlie the pathophysiology affective disorders. The lateral habenula (LHb) has been linked to aversion mood regulation through modulation dopamine serotonin systems. We have defined identity function glutamatergic (Vglut2) control LHb, comparing role inputs originating globus pallidus internal segment (GPi),...
Abstract Dopamine neurons are characterized by their response to unexpected rewards, but they also fire during movement and aversive stimuli. neuron diversity has been observed based on molecular expression profiles; however, whether different functions map onto such genetic subtypes remains unclear. In this study, we established that three dopamine within the substantia nigra pars compacta, of Slc17a6 ( Vglut2 ), Calb1 Anxa1 , each have a unique set responses stimuli accelerations...