A5101935888- Cell Image Analysis Techniques
- Functional Brain Connectivity Studies
- Advanced Fluorescence Microscopy Techniques
- Gene Regulatory Network Analysis
- Single-cell and spatial transcriptomics
- Genomics and Phylogenetic Studies
- Bioinformatics and Genomic Networks
- Medical Imaging Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Advanced MRI Techniques and Applications
- RNA and protein synthesis mechanisms
- Craniofacial Disorders and Treatments
- Cleft Lip and Palate Research
- Gene expression and cancer classification
- Bacterial Genetics and Biotechnology
- Neuroscience and Neuropharmacology Research
- Algal biology and biofuel production
- Neuroendocrine regulation and behavior
- Infant Health and Development
- Female Genital Mutilation/Cutting Issues
- Engineering Technology and Methodologies
- Photosynthetic Processes and Mechanisms
- Memory and Neural Mechanisms
- Neuroinflammation and Neurodegeneration Mechanisms
- Genomics and Chromatin Dynamics
Weill Cornell Medicine
2023
Cornell University
2023
Stanford University
2016-2021
Columbia University
2021
Columbia University Irving Medical Center
2021
Creative Commons
2019
University of Southern California
2019
Howard Hughes Medical Institute
2017
Harvard University
2002-2011
Imaging Center
2008
Transcriptome mapping in the 3D brain RNA sequencing samples entire transcriptome but lacks anatomical information. In situ hybridization, on other hand, can only profile a small number of transcripts. technologies address these shortcomings face challenge dense, complex tissue environments. Wang et al. combined an efficient approach with hydrogel-tissue chemistry to develop multidisciplinary technology for three-dimensional (3D) intact-tissue (see Perspective by Knöpfel). More than 1000...
Brain circuits that modulate sociability Understanding the neural mechanisms mediate social reward has important societal and clinical implications. Hung et al. found release of neuropeptide oxytocin in ventral tegmental area brain increased prosocial behaviors mice (see Perspective by Preston). Optogenetic manipulation influenced a context-dependent manner. Oxytocin activity dopamine cells project to nucleus accumbens, another key node circuitry brain. Science , this issue p. 1406 ; see also 1353
Alterations in the balance between neuronal excitation and inhibition (E:I balance) have been implicated neural circuit activity-based processes that contribute to autism phenotypes. We investigated whether acutely reducing E:I mouse brain could correct deficits social behavior. used mice lacking CNTNAP2 gene, which has autism, achieved a temporally precise reduction medial prefrontal cortex (mPFC) either by optogenetically increasing excitability of inhibitory parvalbumin (PV) neurons or...
The marine cyanobacterium Prochlorococcus MED4 has the smallest genome and cell size of all known photosynthetic organisms. Like phototrophs at temperate latitudes, it experiences predictable daily variation in available light energy which leads to temporal regulation partitioning key cellular processes. To better understand tempo choreography this minimal phototroph, we studied entire transcriptome over a simulated light-dark cycle, placed context diagnostic physiological cycle parameters....
Chromosomes are compacted hundreds of times to fit in the cell, packaged into dynamic folds whose structures largely unknown. Here, we examine patterns gene locations infer large-scale features bacterial chromosomes. Specifically, analyzed >100 genomes and identified thousands pairs that display two types evolutionary correlations: a tendency co-occur be located close together many genomes. We then detailed distribution these Escherichia coli found genes pair tend separated by integral...
Significant advances in system-level modeling of cellular behavior can be achieved based on constraints derived from genomic information and optimality hypotheses. For steady-state models metabolic networks, mass conservation reaction stoichiometry impose linear fluxes. Different objectives, such as maximization growth rate or minimization flux distance a reference state, tested different organisms conditions. In particular, we have suggested that the properties mutant bacterial strains are...
Whole-brain fluorescence images require several stages of computational processing to fully reveal the neuron morphology and connectivity information they contain. However, these tools are rarely part an integrated pipeline. Here we present BrainLine, open-source pipeline that interfaces with existing software provide registration, axon segmentation, soma detection, visualization analysis results. By implementing a feedback based training paradigm were able use single learning algorithm...
Primary cleft rhinoplasty has become widely accepted owing to evidence of improved outcomes and need for fewer revisions. Several techniques have been described, but few surgeons reported long-term repairs performed via a single method. The present study examines surgeon's experience over 22 years using the same primary technique.All consecutive cases by senior author at time unilateral lip repair from 1996 2018 were selected retrospective review. Patients older than 3 or lacking documented...
Abstract Quantifying terabyte-scale multi-modal human and animal imaging data requires scalable analysis tools. We developed CloudReg, an open-source, automatic, terabyte-scale, cloud-based image pipeline that pre-processes registers cross-modal volumetric datasets with artifacts via spatially-varying polynomial intensity transform. CloudReg accurately the following to their respective atlases: in vivo ex macaque brain magnetic resonance imaging, mouse micro-computed tomography, cleared...
Big imaging data is becoming more prominent in brain sciences across spatiotemporal scales and phylogenies. We have developed a computational ecosystem that enables storage, visualization, analysis of these the cloud, thusfar spanning 20+ publications 100+ terabytes including nanoscale ultrastructure, microscale synaptogenetic diversity, mesoscale whole connectivity, making NeuroData largest most diverse open repository data.