A5025099730- Single-cell and spatial transcriptomics
- RNA modifications and cancer
- RNA Research and Splicing
- Integrated Circuits and Semiconductor Failure Analysis
- Cell Image Analysis Techniques
- Advanced biosensing and bioanalysis techniques
- Gene Regulatory Network Analysis
- Molecular Biology Techniques and Applications
- Ion-surface interactions and analysis
- Liver Disease Diagnosis and Treatment
- Genomics and Chromatin Dynamics
- Liver Disease and Transplantation
- Cancer-related gene regulation
- bioluminescence and chemiluminescence research
- Cancer-related molecular mechanisms research
- Liver physiology and pathology
- Acute Lymphoblastic Leukemia research
- Neurogenetic and Muscular Disorders Research
- Genetic factors in colorectal cancer
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Electron Microscopy Techniques and Applications
- Wnt/β-catenin signaling in development and cancer
- RNA and protein synthesis mechanisms
- Fibroblast Growth Factor Research
- Immune Cell Function and Interaction
Baxter (United States)
2016-2023
Stanford University
2016-2023
Institute for Research in Biomedicine
2012-2015
Azienda Ospedaliera Sant'Andrea
2009
Transcriptome mapping in the 3D brain RNA sequencing samples entire transcriptome but lacks anatomical information. In situ hybridization, on other hand, can only profile a small number of transcripts. technologies address these shortcomings face challenge dense, complex tissue environments. Wang et al. combined an efficient approach with hydrogel-tissue chemistry to develop multidisciplinary technology for three-dimensional (3D) intact-tissue (see Perspective by Knöpfel). More than 1000...
Abstract Simultaneous visualization of the relationship between multiple biomolecules and their ligands or small molecules at nanometer scale in cells will enable greater understanding how biological processes operate. We present here high-definition multiplex ion beam imaging (HD-MIBI), a secondary mass spectrometry approach capable high-parameter 3D targeted entities exogenously added structurally-unmodified molecules. With this technology, atomic constituents themselves can be used our...
Abstract The effects exerted by the keratinocyte growth factor (KGF) on intestinal epithelial cells cultured in vitro are influenced cell confluence and differentiation through modulation of receptor (KGFR) expression. In order to better define contribution KGF proliferation, here we developed a coculture model, able mimick epithelial–mesenchymal interactions bowel. consequence its ability produce KGF, demonstrated real‐time PCR Western blot analysis, human colon fibroblast line CCD‐18 has...
Abstract Resistance to glucocorticoids (GC) is associated with an increased risk of relapse in B-cell progenitor acute lymphoblastic leukemia (BCP-ALL). Performing transcriptomic and single-cell proteomic studies healthy progenitors, we herein identify coordination between the glucocorticoid receptor pathway developmental pathways. Healthy pro-B cells most highly express receptor, this expression conserved primary BCP-ALL from patients at diagnosis relapse. In-vitro vivo treatment...
CPEB (Cytoplasmic Polyadenylation Element Binding) proteins are a family of four RNA-binding that regulate the translation maternal mRNAs controlling meiotic cell cycle progression. But CPEBs not limited to transcriptionally silent germline; they also expressed, in various combinations, somatic cells, yet their role regulation mitosis-related gene expression is largely unknown. Deregulation CPEB1 and CPEB4 have been linked tumor development. However, systematic analysis addressing...
Over a decade ago, the Atacama humanoid skeleton (Ata) was discovered in region of Chile. The Ata specimen carried strange phenotype—6-in stature, fewer than expected ribs, elongated cranium, and accelerated bone age—leading to speculation that this preserved nonhuman primate, human fetus harboring genetic mutations, or even an extraterrestrial. We previously reported it by DNA analysis with estimated age about 6–8 yr at time demise. To determine possible drivers observed morphology, from...
Abstract Single-cell omics provide insight into cellular heterogeneity and function. Recent technological advances have accelerated single-cell analyses, but workflows remain expensive complex. We present a method enabling simultaneous, ultra-high throughput barcoding of millions cells for targeted analysis proteins RNAs. Quantum (QBC) avoids isolation single by building cell-specific oligo barcodes dynamically within each cell. With minimal instrumentation (four 96-well plates multichannel...
Abstract To quantify visual and spatial information in single cells with a throughput of thousands per second, we developed Subcellular Localization Assay (SLA). This adaptation Proximity Ligation expands the capabilities flow cytometry to include data relating localization proteins within organelles. We used SLA detect nuclear import transcription factors across cell subsets complex samples. further measured intranuclear re‐localization target cycle upon DNA damage induction. combines...
Abstract Technologies that visualize multiple biomolecules at the nanometer scale in cells will enable deeper understanding of biological processes proceed molecular scale. Current fluorescence-based methods for microscopy are constrained by a combination spatial resolution limitations, limited parameters per experiment, and detector systems wide variety found cells. We present here super-resolution ion beam imaging (srIBI), secondary mass spectrometry approach capable high-parameter 3D...
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