A5039737999- Galectins and Cancer Biology
- Pancreatic and Hepatic Oncology Research
- Wnt/β-catenin signaling in development and cancer
- Glycosylation and Glycoproteins Research
- Signaling Pathways in Disease
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- S100 Proteins and Annexins
- Cancer Research and Treatments
- Phagocytosis and Immune Regulation
- Cancer Cells and Metastasis
- Cancer-related gene regulation
- Kruppel-like factors research
- Skin and Cellular Biology Research
- Peptidase Inhibition and Analysis
- Cancer Immunotherapy and Biomarkers
- Pancreatitis Pathology and Treatment
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Blood Coagulation and Thrombosis Mechanisms
- Cancer Mechanisms and Therapy
- Biomarkers in Disease Mechanisms
- Rheumatoid Arthritis Research and Therapies
- Dermatologic Treatments and Research
Hospital Del Mar
2016-2025
Institut d'Investigacions Biomèdiques de Barcelona
2017-2025
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2020-2025
Hospital del Mar Research Institute
2015-2024
Hospital Universitario de Fuenlabrada
2019-2024
Hospital de Sant Joan Despí Moisès Broggi
2024
Centro de Investigación Biomédica en Red
2024
Universitat Pompeu Fabra
2024
Consejo Superior de Investigaciones Científicas
1994-2022
Luhansk State Medical University
2020
Abstract A substantial proportion of cancer patients do not benefit from platinum-based chemotherapy (CT) due to the emergence drug resistance. Here, we apply elemental imaging mapping CT biodistribution after therapy in residual colorectal and achieve a comprehensive analysis genetic program induced by oxaliplatin-based tumor microenvironment. We show that oxaliplatin is largely retained cancer-associated fibroblasts (CAFs) long time treatment ceased. determine accumulation CAFs intensifies...
The two major cadherins of endothelial cells are neural (N)-cadherin and vascular (VE)- cadherin. Despite similar level protein expression only VE-cadherin is located at cell–cell contacts, whereas N-cadherin distributed over the whole cell membrane. Cotransfection in CHO resulted same distribution as that observed indicating behavior was not specific but related to their structural characteristics. Similar amounts α- β-catenins plakoglobin were associated VE- N-cadherins, p120 higher...
Human vascular endothelial cadherin (VE-cadherin, 7B4/cadherin-5) is an endothelial-specific localized at the intercellular junctions. To directly investigate functional role of this molecule we cloned full-length cDNA from human cells and transfected its coding region into Chinese hamster ovary cells. The product had same molecular weight as natural VE-cadherin in cells, reacted with several mouse monoclonal antibodies. Furthermore, it selectively concentrated junctions, where codistributed...
The expression of the cell-cell adhesion molecules E- and P-cadherin has been analyzed in seven mouse epidermal keratinocyte cell lines representative different stages carcinogenesis. An inverse correlation between amount E-cadherin protein tumorigenicity found, together with a complete absence mRNA three carcinoma (the epithelioid HaCa4 fibroblastoid CarB CarC cells). A similar result detected tumors induced nude mice by lines, where induction takes place moderately differentiated squamous...
Abstract —Endothelial adherens junctions (AJ) promote intercellular adhesion and may contribute to the control of vascular permeability. These structures are formed by a transmembrane cell-specific adhesive protein, endothelial (VE)-cadherin, which is linked its cytoplasmic tail intracellular proteins called catenins (α-catenin, β-catenin, plakoglobin) actin cytoskeleton. Little known about functional regulation AJ in cells. In this study, we analyzed effect histamine on organization...
Vascular endothelial cadherin (VE-cadherin, cadherin-5, or 7B4) is an specific that regulates cell to junction organization in this type. Cadherin linkage intracellular catenins was found be required for their adhesive properties and localization at junctions. We constructed a mutant form of VE-cadherin lacking the last 82 amino acids cytoplasmic domain. Surprisingly, despite any detectable association truncated catenin-cytoskeletal complex, molecule able cluster cell-cell contacts manner...
Endothelial cell proliferation is inhibited by the establishment of to contacts. Adhesive molecules at junctions could therefore play a role in transferring negative growth signals. The transmembrane protein VE-cadherin (vascular endothelial cadherin/cadherin-S) selectively expressed intercellular clefts endothelium. intracellular domain interacts with cytoplasmic proteins called catenins that transmit adhesion signal and contribute anchorage actin cytoskeleton. Transfection both Chinese...
Pancreatic ductal adenocarcinoma (PDA) remains one of the most lethal tumor types, with extremely low survival rates due to late diagnosis and resistance standard therapies. A more comprehensive understanding complexity PDA pathobiology, especially role microenvironment in disease progression, should pave way for therapies improve patient response rates. In this study, we identify galectin-1 (Gal1), a glycan-binding protein that is highly overexpressed stroma, as major driver pancreatic...
Despite some advances, pancreatic ductal adenocarcinoma (PDAC) remains generally refractory to current treatments. Desmoplastic stroma, a consistent hallmark of PDAC, has emerged as major source therapeutic resistance and thus potentially promising targets for improved treatment. The glycan-binding protein galectin-1 (Gal1) is highly expressed in PDAC but its roles there have not been studied. Here we report functions molecular pathways Gal1 that mediate oncogenic properties this setting....
CD69 is involved in immune cell homeostasis, regulating the T cell-mediated response through control of Th17 differentiation. However, natural ligands for have not yet been described. Using recombinant fusion proteins containing extracellular domain CD69, we detected presence a ligand(s) on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses CD69-binding moieties DCs identified galectin-1 as counterreceptor. Surface plasmon resonance and anti-CD69 blocking...
ABSTRACT Vascular endothelial cells assemble adhesive intercellular junctions comprising a unique cadherin, VE-cadherin, which is coupled to the actin cytoskeleton through cytoplasmic interactions with plakoglobin, β-catenin and α-catenin. However, potential linkage between VE-cadherin vimentin intermediate filament not well characterized. Recent evidence indicates that lymphatic vascular express desmoplakin, desmosomal protein attaches filaments plasma membrane in epithelial cells. In...
High levels of S100A6 have been associated with poor outcome in pancreatic cancer patients. The functional role is, however, poorly understood. Immunoprecipitation followed by two-dimensional gel electrophoresis and mass spectrometry were undertaken to identify interacting proteins cells. Immunohistochemistry coimmunofluorescence performed examine expression or colocalisation proteins. siRNA was used deplete specific effects on motility measured using Boyden Chamber wound healing assays. Our...
Abstract Background A role for the innate immune system in driving autoimmune T cell cascade psoriasis has been proposed. Toll‐like receptors‐(TLR)‐2 and ‐4 play a inflammation, atherosclerosis, their specific remains unclear. Objective To evaluate TLR2 TLR4 gene expression levels peripheral blood mononuclear cells from psoriatic patients. Methods Changes TLR2/4 expressions were evaluated using quantitative real‐time reverse transcription polymerase chain reaction cells, twenty‐one patients...
The impact of RNA structures in coding sequences (CDS) within mRNAs is poorly understood. Here, we identify a novel and highly conserved mechanism translational control involving the DEAD-box helicase Dhh1. Using yeast genetics genome-wide ribosome profiling analyses, show that this mechanism, initially derived from studies Brome Mosaic virus genome, extends to human enriched membrane secreted proteins. All Dhh1-dependent mRNAs, viral cellular, share key common features. First, they contain...