A5021465541- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immunotherapy and Immune Responses
- Galectins and Cancer Biology
- Psoriasis: Treatment and Pathogenesis
- COVID-19 Clinical Research Studies
- Dermatology and Skin Diseases
- SARS-CoV-2 and COVID-19 Research
- Cell Adhesion Molecules Research
- Asthma and respiratory diseases
- Pneumonia and Respiratory Infections
- Atherosclerosis and Cardiovascular Diseases
- MicroRNA in disease regulation
- Signaling Pathways in Disease
- Immune Response and Inflammation
- IL-33, ST2, and ILC Pathways
- Long-Term Effects of COVID-19
- Toxin Mechanisms and Immunotoxins
- Extracellular vesicles in disease
- Inflammasome and immune disorders
- Pneumocystis jirovecii pneumonia detection and treatment
- Systemic Lupus Erythematosus Research
- Nuclear Receptors and Signaling
- Rheumatoid Arthritis Research and Therapies
- Cardiac Fibrosis and Remodeling
Hospital Universitario de La Princesa
2016-2025
Centro de Investigación en Red en Enfermedades Cardiovasculares
2018-2024
Universidad Autónoma de Madrid
2013-2023
Instituto de Salud Carlos III
2016-2023
Spanish National Centre for Cardiovascular Research
2012-2023
Centro de Investigación Biomédica en Red
2017-2022
Trakya University
2016
Autonomous University of San Luis Potosí
1998-2003
Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán
2000-2001
California Polytechnic State University
2000
SARS-CoV-2 is responsible for the development of coronavirus disease 2019 (COVID-19) in infected individuals, who can either exhibit mild symptoms or progress toward a life-threatening acute respiratory distress syndrome (ARDS). Exacerbated inflammation and dysregulated immune responses involving T myeloid cells occur COVID-19 patients with severe clinical progression. However, differential contribution specific subsets dendritic monocytes to ARDS still poorly understood. In addition, role...
The diagnosis of acute myocarditis typically requires either endomyocardial biopsy (which is invasive) or cardiovascular magnetic resonance imaging not universally available). Additional approaches to are desirable. We sought identify a novel microRNA for the myocarditis.To specific myocarditis, we performed microarray analyses and quantitative polymerase-chain-reaction (qPCR) assays in sorted CD4+ T cells type 17 helper (Th17) after inducing experimental autoimmune myocardial infarction...
T regulatory cells have a key role in the pathogenesis of autoimmune diseases different animal models. However, less information is available regarding these human thyroid (AITD).The objective study was to analyze cell subsets patients with AITD.We studied by flow cytometry and immunohistochemistry peripheral blood mononuclear (PBMCs) infiltrates from 20 AITD. In addition, function T(REG) lymphocytes assessed proliferation assays. Finally, TGF-beta mRNA tissue its vitro synthesis (TMCs)...
Extracellular vesicles (EVs) are emerging as potent non-invasive biomarkers. However, current methodologies time consuming and difficult to translate clinical practice. To analyse EV-encapsulated circulating miRNA, we searched for a quick, easy economic method enrich frozen human serum samples EV. We compared the efficiency of several protocols commercial kits isolate EVs. Different methods based on precipitation, columns or filter systems were tested with ultracentrifugation, which is most...
The increase and persistence of inflammation in community-acquired pneumonia (CAP) patients can lead to higher mortality. Biomarkers capable measuring this inadequate inflammatory response are likely candidates be related with a bad outcome. We investigated the association between concentrations several markers mortality CAP patients.This was prospective study hospitalised Spanish university hospital. Blood tests upon admittance early-stage evolution (72-120 hours) were carried out, where...
Coronavirus Disease 2019 (COVID-19) pneumonia is a life-threatening infectious disease, especially for elderly patients with multiple comorbidities. Despite enormous efforts to understand its underlying etiopathogenic mechanisms, most of them remain elusive. In this study, we compared differential plasma miRNAs and cytokines profiles between COVID-19 other community-acquired pneumonias (CAP). A first screening subsequent validation assays in an independent cohort revealed signature 15...
CD69 is induced after activation of leukocytes at inflammatory sites, but its physiological role during inflammation remains unknown. We explored the in autoimmune reactivity by analyzing a model collagen-induced arthritis (CIA) WT and CD69-deficient mice. CD69–/– mice showed higher incidence severity CIA, with exacerbated T B cell immune responses to type II collagen. Levels TGF-β1 TGF-β2, which act as protective agents were reduced foci, correlating increase proinflammatory cytokines IL-1β...
CD69 is induced after activation of leukocytes at inflammatory sites, but its physiological role during inflammation remains unknown. We explored the in autoimmune reactivity by analyzing a model collagen-induced arthritis (CIA) WT and CD69-deficient mice. CD69–/– mice showed higher incidence severity CIA, with exacerbated T B cell immune responses to type II collagen. Levels TGF-β1 TGF-β2, which act as protective agents were reduced foci, correlating increase proinflammatory cytokines IL-1β...
CD69 is involved in immune cell homeostasis, regulating the T cell-mediated response through control of Th17 differentiation. However, natural ligands for have not yet been described. Using recombinant fusion proteins containing extracellular domain CD69, we detected presence a ligand(s) on human dendritic cells (DCs). Pulldown followed by mass spectrometry analyses CD69-binding moieties DCs identified galectin-1 as counterreceptor. Surface plasmon resonance and anti-CD69 blocking...
Antigen-specific cognate interaction of T lymphocytes with antigen-presenting cells (APCs) drives major morphological and functional changes in cells, including actin rearrangements at the immune synapse (IS) formed cell-cell contact area. Here we show, using cell lines as well primary that clathrin, a protein involved endocytic processes, accumulation IS. Clathrin is recruited towards IS parallel kinetics to actin. Knockdown clathrin prevents proteins polymerization, such dynamin-2, Arp2/3...
BackgroundPsoriasis is a frequent inflammatory skin disease that mainly mediated by IL-23, IL-1β, and IL-17 cytokines. Although psoriasis hyperproliferative disorder, the possible role of amino acid transporters has remained unexplored.ObjectiveWe sought to investigate essential transporter L-type (LAT) 1 (SLC7A5) in psoriasis.MethodsLAT1 floxed mice were crossed Cre-expressing mouse strains under control keratin 5, CD4, retinoic receptor–related orphan receptor γ. We produced models...
Increasing evidence has pointed to the important function of T cells in controlling immune homeostasis and pathogenesis after myocardial infarction (MI), although underlying molecular mechanisms remain elusive. In this study, a broad analysis markers 283 patients revealed significant CD69 overexpression on Tregs MI. Our results mice showed that expression increased survival left anterior descending (LAD) coronary artery ligation. Cd69–/– developed strong IL-17+ γδT cell responses ischemia...
SUMMARY The aim of this work was to investigate the effect cadmium, lead and arsenic on apoptosis human immune cells. Peripheral blood mononuclear cells (MNC) were incubated with increasing concentrations these metals then cellular determined by flow cytometry DNA electrophoresis. We found that induced a significant level at 15 μm after 48h incubation. Cadmium had similar effect, but higher (65 μm). In addition, cadmium exerted cytotoxic MNC seemed be independent induction apoptosis....
We have explored the therapeutic potential of statins in patients with different inflammatory rheumatic diseases refractory to conventional therapy. found that simvastatin (80mg o.d. for eight days) induced a rapid and significant reduction proteinuria levels three systemic lupus erythematosus (SLE) patients. A similar kind therapy had marked beneficial effect patient Wegener's granulomatosis erythema nodosum. On other hand, five rheumatoid arthritis (RA) who received atorvastatin days...
Abstract Dendritic cells (DCs) have a key role in both the generation of immune response and induction tolerance to self-Ags. In this work, possible P-selectin glycoprotein ligand 1 (PSGL-1) on tolerogenic activity human DCs was explored. We found that engagement PSGL-1 by induced expression c-Fos, IDO, IL-10, TGF-β genes. Remarkably, stimulation through with enhanced their capability generate CD4+CD25+Foxp3+ regulatory T cells, which expressed high levels TGF-β1 mRNA, synthesized suppressed...
Although the role of Th17 and regulatory T cells in progression atherosclerosis has been highlighted recent years, their molecular mediators remain elusive. We aimed to evaluate association between CD69 receptor, a regulator Th17/regulatory cell immunity, development animal models patients with subclinical disease.Low-density lipoprotein receptor-deficient chimeric mice expressing or not on either myeloid lymphoid were subjected high fat diet. In vitro functional assays human performed...
The aim of this study was to identify a novel immunological indicator useful for the early diagnosis (through rapid and single determination) neonatal sepsis (NS). Peripheral blood samples were taken from 63 neonates, who classified into four groups: proven NS (n = 17); clinical 14); disease without infection healthy newborns 15). Neutrophil expression CD64, CD43, CD44, CD50, CD62L Mac‐1, plasma levels interleukin (IL)‐1β, IL‐6, tumor necrosis factor‐α (TNF‐α) soluble L ‐selectin (sCD62L),...