A5004962196- Metabolomics and Mass Spectrometry Studies
- Liver Disease Diagnosis and Treatment
- Bioinformatics and Genomic Networks
- Cancer, Hypoxia, and Metabolism
- Mitochondrial Function and Pathology
- Cancer, Lipids, and Metabolism
- Advanced Proteomics Techniques and Applications
- Chronic Obstructive Pulmonary Disease (COPD) Research
- RNA modifications and cancer
- Chronic Lymphocytic Leukemia Research
- Psoriasis: Treatment and Pathogenesis
- Microbial Metabolic Engineering and Bioproduction
- Dermatology and Skin Diseases
- Congenital heart defects research
- Sphingolipid Metabolism and Signaling
- IL-33, ST2, and ILC Pathways
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Caveolin-1 and cellular processes
- Medical and Biological Ozone Research
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Angiogenesis and VEGF in Cancer
- Extracellular vesicles in disease
- Analytical Chemistry and Sensors
- Obstructive Sleep Apnea Research
Spanish National Centre for Cardiovascular Research
2016-2025
Georgetown University
2015-2019
Georgetown University Medical Center
2015-2019
Universidad San Pablo CEU
2010-2017
University of Modena and Reggio Emilia
2010
The composition and physical properties of the extracellular matrix (ECM) critically influence tumor progression, but molecular mechanisms underlying ECM layering are poorly understood. Tumor–stroma interaction depends on cell communication mediated by exosomes, small vesicles generated within multivesicular bodies (MVBs). We show that caveolin-1 (Cav1) centrally regulates exosome biogenesis exosomal protein cargo sorting through control cholesterol content at endosomal compartment/MVBs....
This study evaluates changes in metabolite levels hepatocellular carcinoma (HCC) cases vs. patients with liver cirrhosis by analysis of human blood plasma using gas chromatography coupled mass spectrometry (GC-MS). Untargeted metabolomic samples from participants recruited Egypt was performed two GC-MS platforms: a GC to single quadruple spectrometer (GC-qMS) and time-of-flight (GC-TOFMS). Analytes that showed statistically significant ion intensities were selected ANOVA models. These...
Cardiac steatosis and apoptosis are key processes in diabetic cardiomyopathy, but the underlying mechanisms have not been elucidated, leading to a lack of effective therapy. The mineralocorticoid receptor blocker, eplerenone, has demonstrated anti-fibrotic actions heart. However, its effects on fatty-acid accumulation apoptotic responses revealed.Non-hypertensive Zucker Diabetic Fatty (ZDF) rats received eplerenone (25 mg/kg) or vehicle. Lean (ZL) were used as control (n = 10, each group)....
Hepatocellular carcinoma (HCC) causes more than half a million annual deaths worldwide. Understanding the mechanisms contributing to HCC development is highly desirable for improved surveillance, diagnosis, and treatment. Liver tissue metabolomics has potential reflect physiological changes behind development. Also, it allows identification of biomarker candidates future evaluation in biofluids investigation racial disparities HCC. Tumor nontumor tissues from 40 patients were analyzed by...
Abstract Background: Metabolomics plays an important role in providing insight into the etiology and mechanisms of hepatocellular carcinoma (HCC). This is accomplished by a comprehensive analysis patterns involved metabolic alterations human specimens. study compares levels plasma metabolites HCC cases versus cirrhotic patients evaluates ability candidate distinguishing two groups. Also, it investigates combined use clinical covariates for detection with liver cirrhosis. Methods: Untargeted...
Ozonated autohemotherapy (O3-AHT) is a medical approach during which blood obtained from the patient ozonated and injected back into body. Despite an increasing number of evidence that O3-AHT safe, this type therapy remains controversial. To extend knowledge about changes in evoked by O3-AHT, LC–MS- GC–MS-based metabolic fingerprinting was used to compare plasma samples before after treatment with potentially therapeutic concentrations ozone. The procedure performed PVC bags utilized for...
Aims: A variety of vessels, such as resistance pulmonary arteries (PA) and fetoplacental the ductus arteriosus (DA) are specialized in sensing responding to changes oxygen tension. Despite opposite stimuli, normoxic DA contraction hypoxic PA vasoconstriction share some mechanistic features. Activation neutral sphingomyelinase (nSMase) subsequent ceramide production has been involved (HPV). Herein we aimed study possible role nSMase-derived a common factor acute oxygen-sensing function...
Sleep apnea and hypopnea syndrome ( SAHS ) is a multicomponent disorder, with associated cardiovascular metabolic alterations, second in order of frequency among respiratory disorders. diagnosed an overnight sleep test called polysomnogram, which requires having the patient hospital. In addition, more clear classification patients according to mild severe presentations would be desirable. The aim present study was assess relative changes identify new potential biomarkers for diagnosis, able...
Background Cardiovascular (CV) disease is the leading cause of death in women. Although 80% CV events can be prevented, mortality projected to increase, particularly young Objectives To promote health women and encourage appropriate lifestyle changes by increasing awareness through vascular ultrasound imaging. Methods WAKE UP a prospective case-control study target population 720 asymptomatic women, aged 40–70 years with ≥1 major risk factor (RF). Participants will attend baseline visit...
Disparities in hepatocellular carcinoma (HCC) incidence and survival have been observed between ethnic groups including African-Americans (AA) European-Americans (EA). The evaluation of the changes levels metabolites samples stratified by race could provide a snapshot ethnically diverse disease related pathways identify reliable biomarkers. In this study, we considered AA EA to investigate that may be associated with HCC race-specific manner. 46 plasma samples, collected from patients...
HIF1-alpha expression defines metabolic compartments in the developing heart, promoting glycolytic program compact myocardium and mitochondrial enrichment trabeculae. Nonetheless, its role cardiogenesis is debated. To assess importance of during heart development influence glycolysis ventricular chamber formation, herein we generated conditional knockout models Hif1a Nkx2.5 cardiac progenitors cardiomyocytes. Deletion impairs embryonic without influencing cardiomyocyte proliferation results...
Cell proteostasis includes gene transcription, protein translation, folding of de novo proteins, post-translational modifications, secretion, degradation and recycling. By profiling the proteome extracellular vesicles (EVs) from T cells, we have found chaperonin complex CCT, involved in correct particular proteins. limiting CCT cell-content by siRNA, cells undergo altered lipid composition metabolic rewiring towards a lipid-dependent metabolism, with increased activity peroxisomes...
In chronic lymphocytic leukaemia (CLL), the clinical course of patients is heterogeneous. Some present an aggressive disease onset and require immediate therapy, while others remain without treatment for years. Current staging systems developed by Rai Binet may be useful in forecasting patient survival time, but do not discriminate between stable progressive forms early stages. Recently ample attention has been directed towards identifying new prognostic markers capable predicting...
Acute respiratory distress syndrome (ARDS) is a serious complication of influenza A (H1N1) virus infection. Its pathogenesis unknown and biomarkers are lacking. Untargeted metabolomics allows the analysis whole metabolome in biological compartment, identifying patterns associated with specific conditions. We hypothesized that LC-MS could help identify discriminant metabolites able to define metabolic alterations occurring patients infection developed ARDS. Serum samples from diagnosed 2009...
Microvascular dysfunction and resulting tissue hypoxia is a major contributor to the pathogenesis evolution of cardiovascular diseases (CVD). Diverse gene cell therapies have been proposed preserve microvasculature or boost angiogenesis in CVD, with moderate benefit. This study tested vivo impact sequential delivery by bone-marrow (BM) cells pro-angiogenic factors vascular endothelial growth factor (VEGFA) sphingosine-1-phosphate (S1P) myocardial infarction model. For that, mouse BM were...
The threat of Hepatocellular Carcinoma (HCC) is a growing problem, with incidence rates anticipated to near double over the next two decades. increasing burden makes discovery novel diagnostic, prognostic, and therapeutic biomarkers distinguishing HCC from underlying cirrhosis significant focus. In this study, we analyzed tissue serum samples 40 cases 25 patients liver (CIRR) better understand mechanistic differences between CIRR. Through pathway network analysis, are able take systems...
Untargeted metabolomics aims at measuring the entire set of metabolites in a wide range biological samples. However, due to high chemical diversity that from small large and more complex molecules (i.e., amino acids/carbohydrates vs. phospholipids/gangliosides), identification characterization metabolome remain major bottleneck. The first step this process consists searching experimental monoisotopic mass against databases, thus resulting highly redundant/complex list candidates. Despite...
Multi-omic approaches offer the opportunity to characterize complex diseases such as cancer at various molecular levels. In this paper, we present transcriptomic, proteomic/glycoproteomic, glycomic, and metabolomic (TPGM) data acquired by analysis of liver tissues from hepatocellular carcinoma (HCC) cases patients with cirrhosis. We evaluated changes in levels transcripts, proteins, glycans, metabolites between tumor cirrhotic statistical methods. demonstrated potential multi-omic network...