A5032626714- Cardiac Valve Diseases and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Aortic Disease and Treatment Approaches
- Atrial Fibrillation Management and Outcomes
- Venous Thromboembolism Diagnosis and Management
- Electrospun Nanofibers in Biomedical Applications
- Wound Healing and Treatments
- Mesenchymal stem cell research
- Lipoproteins and Cardiovascular Health
- Angiogenesis and VEGF in Cancer
- Tissue Engineering and Regenerative Medicine
- Lipid metabolism and disorders
- Adipokines, Inflammation, and Metabolic Diseases
- Coronary Interventions and Diagnostics
- Peripheral Artery Disease Management
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Galectins and Cancer Biology
- IL-33, ST2, and ILC Pathways
- Hormonal Regulation and Hypertension
- Infective Endocarditis Diagnosis and Management
- Muscle metabolism and nutrition
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Diabetes Treatment and Management
- Dermatological and Skeletal Disorders
- Peptidase Inhibition and Analysis
Universidad Publica de Navarra
2020-2025
Navarrabiomed
2020-2025
Navarre Institute of Health Research
2020-2025
Complejo Hospitalario Universitario de Toledo
2025
University of Bristol
2017-2024
Centre Hospitalier Régional et Universitaire de Nancy
2024
Bristol Royal Infirmary
2017-2024
Complejo Hospitalario de Navarra
2020-2022
Bristol Regional Medical Center
2020
Yale University
1988-2019
Background. Keratinocyte cultures have been used for the treatment of severe burn patients. Here, we describe a new cultured bioengineered skin based on (1) keratinocytes and fibroblasts obtained from single biopsy (2) dermal matrix human plasma. A high expansion capacity achieved by grown this plasma-based is reported. In addition, results successful preclinical clinical tests are presented. Methods. Keratinocytes were double enzymatic digestion (trypsin collagenase, respectively). setting,...
Vascular smooth muscle cells (VSMCs) transdifferentiate into osteoblast-like during vascular cal-cification, inducing active remodeling and calcification of the extracellular matrix (ECM). Intracellular enzymes, such as lysyl hydroxylase 1 (PLOD1) oxidase (LOX), contribute to ECM maturation stabilization. We assessed contribution these enzymes hyperphosphatemia-induced calcification. Human murine VSMCs were differentiated functional by high-phosphate medium (HPM) conditioning. HPM promoted...
Two previous uncontrolled studies have suggested that noninvasive mechanical ventilation (NIMV) in patients with hypercapnic chronic obstructive pulmonary disease (COPD) improves arterial blood gas tensions by decreasing lung hyperinflation the consequent reduction inspiratory loads and changes ventilatory pattern. The aim of this randomised placebo-controlled study was to determine whether these mechanisms play a pivotal role effects NIMV on gases. Thirty-six stable COPD were randomly...
Abstract Remodelling in the atria could appear as a result of hypertension, diabetes or ischaemic heart disease. Galectin-3 (Gal-3) is mediator profibrotic pathways and potential biomarker cardiac remodelling. We prospectively recruited consecutive patients undergoing elective surgery. Preoperative Gal-3 levels were determined from serum samples, presence fibrosis was assessed atrial appendage tissue samples obtained during included 100 with aortic valve diseases 15 controls permanent AF....
Graphene represents one of the most interesting additions to tissue engineering toolbox. Novel graphene-based composites are required improve beneficial graphene properties in terms tridimensional polymeric structure, conferring a higher mechanical strength and favoring differentiation human mesenchymal stem cells. Here, we have demonstrated wide range composite combinations, successful use silk-fibroin constructs for future bioengineering applications field clinical regenerative dentistry...
The Ewing family of tumors harbors chromosomal translocations that join the N-terminal region EWS gene with C-terminal several transcription factors ETS family, mainly FLI1, resulting in chimeric play a pivotal role pathogenesis tumors. To identify downstream targets EWS/FLI1 fusion protein, we established 293 cells expressing constitutively either or wild type FLI1 proteins and used cDNA arrays to genes differentially regulated by EWS/FLI1. DAX1 (NR0B1), an unusual orphan nuclear receptor...
Background and Purpose— Oral anticoagulation is highly effective in reducing stroke mortality atrial fibrillation (AF). Several risk stratification schemes have been developed using clinical characteristics. Elevated levels of N-terminal pro–B-type natriuretic peptide (NT-proBNP) are important markers increased morbidity congestive heart failure general community population. The aim our study was to assess the predictive value NT-proBNP an unselected real-world cohort anticoagulated patients...
Background: Vascular endothelial cells activation and dysfunction mediate inflammation abnormal coagulation in COVID-19 patients. Mineralocorticoid receptor (MR) signaling its downstream target Galectin-3 (Gal-3) are known to cardiovascular might be involved the pathogenesis of complications. Accordingly, we aimed investigate potential beneficial effects MR antagonism Gal-3 inhibition on inflammatory response induced by SARS-CoV-2 Spike protein human aortic (HAECs). Methods: HAECs were...
We aim to analyse sex-specific differences in aortic valves (AVs) and valve interstitial cells (VICs) from stenosis (AS) patients.238 patients with severe AS undergoing surgical replacement were recruited. Two hundred two AVs (39.1% women) used for ex vivo analyses 36 (33.3% vitro experiments. men presented increased levels of the inflammatory molecules interleukin (IL)-1β, IL-6, Rantes, CD45. Oxidative stress (eNOS, myeloperoxidase, malondialdehyde nitrotyrosine) was upregulated male AVs....
Background: Aortic regurgitation (AR) is more prevalent in male, although cellular and molecular mechanisms underlying the sex differences prevalence pathophysiology are unknown. Objectives: This study evaluates impact of on aortic valve (AV) inflammation remodeling as well valvular interstitial cells (VICs) endothelial (VECs) patients with AR. Methods: A total 144 (27.5% female) severe chronic AR were included. AVs analyzed by imaging, histological biology techniques (ELISA, RT-PCR). VICs...
Diabetes mellitus (DM) increases the risk of aortic stenosis (AS) and worsens its pathophysiology in a sex-specific manner. Aldosterone/mineralocorticoid receptor (Aldo/MR) pathway participates early stages AS other diabetic-related cardiovascular complications. We aim to identify new Aldo/MR targets complicated with DM. performed discovery studies using Olink Proteomics® technology 87 patient-derived valves (AVs) (N=28 N=19 non-diabetic diabetic men; N=32 N=8 women, respectively) human...
Background Cardiovascular (CV) disease is the leading cause of death in women. Although 80% CV events can be prevented, mortality projected to increase, particularly young Objectives To promote health women and encourage appropriate lifestyle changes by increasing awareness through vascular ultrasound imaging. Methods WAKE UP a prospective case-control study target population 720 asymptomatic women, aged 40–70 years with ≥1 major risk factor (RF). Participants will attend baseline visit...
Adipose tissue is present in aortic valves (AVs). Valve interstitial cells (VICs) could differentiate into adipogenic lineages. We here characterize whether the presence of adipose AV influences inflammation and extracellular matrix (ECM) composition patients with regurgitation (AR). A total 144 AVs were analyzed by histological molecular techniques. performed discovery studies using Olink Proteomics® technology 40 (N = 16 without N 24 tissue). In vitro, human white adipocytes (HWAs) or VICs...
Using a recently described skin-humanized model based on the engraftment of human bioengineered skin equivalents onto immunodeficient mice, we compared efficacy different in vivo gene transfer strategies aimed at delivering growth factors to promote wound healing. The approaches involving transient delivery keratinocyte factor (KGF) wounds performed engrafted included (1) KGF by intradermal adenoviral injection; (2) vector immobilized fibrin carrier; and (3) KGF-adenoviral gene-transferred...
Abstract Cutaneous diabetic wounds greatly affect the quality of life patients, causing a substantial economic impact on healthcare system. The limited clinical success conventional treatments is mainly attributed to lack knowledge pathogenic mechanisms related chronic ulceration. Therefore, management ulcers remains challenging issue. Within this context, reliable animal models that recapitulate situations impaired wound healing have become essential. In study, we established new in vivo...
MicroRNAs regulate endothelial function and angiogenesis, but their implication in pericyte biology remains undetermined. A PCR array, covering a panel of 379 human microRNAs, showed microRNA-532-5p to be one the most differentially modulated by hypoxia, which was confirmed qPCR both skeletal muscle adventitial pericytes. Furthermore, upregulated murine muscular pericytes early after experimentally induced ischemia, decreasing below baseline reperfusion. Transfection with anti-microRNA,...