A5079162489- Endodontics and Root Canal Treatments
- Bone Tissue Engineering Materials
- Advanced Drug Delivery Systems
- Dental materials and restorations
- Ionic liquids properties and applications
- Analytical Chemistry and Chromatography
- Periodontal Regeneration and Treatments
- Nanocomposite Films for Food Packaging
- Aquaculture disease management and microbiota
- Electrochemical Analysis and Applications
- Polymer Surface Interaction Studies
- Hydrogels: synthesis, properties, applications
- Invertebrate Immune Response Mechanisms
- Nanoparticles: synthesis and applications
- Aquaculture Nutrition and Growth
- Mesenchymal stem cell research
- Dental Trauma and Treatments
- Sirtuins and Resveratrol in Medicine
- Dental Erosion and Treatment
- Polysaccharides and Plant Cell Walls
- Silk-based biomaterials and applications
- Chemical and Physical Properties in Aqueous Solutions
- Salivary Gland Disorders and Functions
- Cancer-related Molecular Pathways
- Coagulation and Flocculation Studies
Universidad de Murcia
2012-2025
University of Leeds
2019-2023
University of Coimbra
2020-2023
Universitat de València
2019
Hospital Universitario Virgen de la Arrixaca
2019
Instituto Murciano de Investigación Biosanitaria
2018
Abstract Aims To investigate the cytotoxicity and bioactivity of several pulpotomy materials: Biodentine (Septodont, Saint‐Maur‐des‐Fosses, France) MTA (Angelus, Londrina, PR , Brazil), Theracal LC (Bisco Inc., Schamburg, IL USA ) IRM (Dentsply DeTrey GmbH, Konstanz, Germany), after contact with stem cells isolated from human exfoliated primary teeth ( SHED s). Methodology s were cultured in presence eluates various materials for 24, 48 72 h. Cell viability was determined by mitochondrial...
Abstract Aim To evaluate the biological effects in vitro of MTA‐Angelus (MTA‐Ang; Angelus, Londrina, PR, Brazil), MTA Repair HP (MTA‐HP; Angelus) and NeoMTA Plus (NeoMTA‐P; Avalon Biomed Inc, Bradenton, FL, USA) on human dental pulp stem cells ( h DPSCs). Methodology Cell viability cell migration assays were performed using eluates each material. morphology attachment to different materials, hDPSCs directly seeded onto material surfaces analysed by immunocytofluorescence scanning electron...
To evaluate the biocompatibility of three calcium silicate-based endodontic sealers, Bioroot BC Sealer (Septodont, Saint-Maur-des-Fosses, France), Endoseal MTA (EndoSeal, Maruchi, Seoul, Korea) and Nano-ceramic (B&L Biotech, Fairfax, VA, USA) (NCS), on human periodontal ligament stem cells (hPDLSCs).Human were cultured in presence various sealer eluates for 24 h. Cell viability was determined using MTT assay. death changes phenotype induced by set evaluated through flow cytometry. Also, an...
Graphene represents one of the most interesting additions to tissue engineering toolbox. Novel graphene-based composites are required improve beneficial graphene properties in terms tridimensional polymeric structure, conferring a higher mechanical strength and favoring differentiation human mesenchymal stem cells. Here, we have demonstrated wide range composite combinations, successful use silk-fibroin constructs for future bioengineering applications field clinical regenerative dentistry...
Size-exclusion chromatography (SEC) is presently a widely used and very informative technique for the characterization of macromolecules in solution. Beyond first implementations SEC-which required cumbersome column calibrations were mainly intended determination molecular weights-the modern SEC approach involving multiple detectors (md-SEC) based on solution properties such as intrinsic viscosity light scattering. Thus, md-SEC enables direct more efficient weights, well relationships...
Abstract Multiple side effects related to bleaching were found occur in the dental pulp tissue, including decreased cell metabolism and viability. In this work we evaluated vitro diffusion capacity, cytotoxicity biocompatibility of four commercial products on stem cells from human ( h DPSCs). Two gels hydrogen peroxide-based (HP), Norblanc Office 37.5% (Nor-HP) Opalescence Boost 40% (Opal-HP) applied for 30 min enamel/dentine discs. Another two same manufacturers, 16% carbamide (CP), Home...
Fumonisin B1 (FB1), a mycotoxin classified as group 2B hazard, is of high importance due to its abundance and occurrence in varied crops. Conventional methods for detection are sensitive selective; however, they also convey disadvantages such long assay times, expensive equipment instrumentation, complex procedures, sample pretreatment unfeasibility on-site analysis. Therefore, there need quick, simple affordable quantification methods. On that note, aptamers (ssDNA) good alternative...
The study of mucosal surfaces, and in particular the fish skin its secreted mucus, has been great interest recently among immunologists. Measurement viscosity other physico-chemical parameters (protein concentration, pH, conductivity, redox potential, osmolality density) mucus can help to understand biological functions. We have used five marine species teleost: gilthead seabream (Sparus aurata L.), European sea bass (Dicentrarchus labrax shi drum (Umbrina cirrosa common dentex (Dentex L.)...
Abstract Aim To analyse in vitro changes ion release and biological properties of Endocem‐ MTA (Maruchi, Wonju, Korea) Neo ‐Plus (Avalon Biomed Inc, Bradenton, FL , USA ) exposed to acidic or neutral environment on human dental periodontal ligament stem cells ( h PDLSC s). Methodology Cell viability wound healing assays were performed using eluates each material. death phenotype induced by the set endodontic sealer evaluated through flow cytometry. evaluate cell attachment different...
Polyelectrolyte nanocomposites rarely reach a stable state and aggregation often occurs. Here, we report the synthesis of for oral delivery insulin composed alginate, dextran sulfate, poly-(ethylene glycol) 4000, poloxamer 188, chitosan, bovine serum albumin. The were obtained by Ca2+-induced gelation alginate followed an electrostatic-interaction process among polyelectrolytes. Chitosan seemed to be essential final size there was optimal content that led 400-600 nm hydrodynamic size....