A5024142652- Polysaccharides Composition and Applications
- Proteins in Food Systems
- Advanced Drug Delivery Systems
- Polysaccharides and Plant Cell Walls
- Nanocomposite Films for Food Packaging
- Surfactants and Colloidal Systems
- Hydrogels: synthesis, properties, applications
- Polymer Surface Interaction Studies
- Bacterial biofilms and quorum sensing
- Advanced biosensing and bioanalysis techniques
- Protein Hydrolysis and Bioactive Peptides
- Biosensors and Analytical Detection
- Seaweed-derived Bioactive Compounds
- Antimicrobial Peptides and Activities
- Lipid Membrane Structure and Behavior
- RNA Interference and Gene Delivery
- Microfluidic and Bio-sensing Technologies
- Botanical Research and Applications
- Microbial Metabolites in Food Biotechnology
- Food composition and properties
- Algal biology and biofuel production
- Helicobacter pylori-related gastroenterology studies
- Nanoparticle-Based Drug Delivery
- Electrostatics and Colloid Interactions
- biodegradable polymer synthesis and properties
University of Leeds
2016-2024
Universidad de Murcia
2023-2024
University of Münster
2013-2022
Universidade de Santiago de Compostela
2007-2016
Institute of Plant Biology and Biotechnology
2016
Centro de Investigación en Alimentación y Desarrollo
2002-2015
CeNTech
2015
Universidad de Hermosillo
2011-2015
Universidad de Sonora
2003-2014
Universidad Complutense de Madrid
2007-2011
Chitosan nanoparticles, produced by ionic gelation, are among the most intensely studied nanosystems for drug delivery. However, a lack of inter-laboratory reproducibility and poor physicochemical understanding process particle formation have been slowing their potential market applications. To address these shortcomings, current study presents systematic analysis main polymer factors affecting nanoparticle driven an initial screening using statistical Design Experiments (DoE). In summary,...
Nanoparticles intended for use in the transmucosal delivery of macromolecules were prepared by ionic gelation chitosan (CS) hydrochloride with pentasodium tripolyphosphate (TPP) and concomitant complexation sodium alginate (ALG). The incorporation a small proportion ALG increasing molecular weight (M(w); from 4 to 74 kDa) into nanoparticles led monotonic increase colloidal size ∼260 ∼525 nm. This was regarded as consequence formation gradually more expanded structures. Insulin, taken model...
Abstract BACKGROUND: Chitosan can form antimicrobial, semi‐permeable barriers that limit gas exchange and reduces water loss in fruits. Consumer interest fresh‐cut papaya fruit is leading to increasing demand because of its sensorial antioxidant properties. However, a highly perishable product prone weight, firmness microbial attack. The aim this study was evaluate the effect chitosan coatings on overall quality papaya. low (LMWC), medium (MMWC) high (HMWC) molecular weights, at...
To evaluate the antibacterial and free-radical scavenging (FRS) activities of propolis collected from three different areas Sonoran Desert in northwestern Mexico [Pueblo de Alamos (PAP), Ures (UP) Caborca (CP)].The FRS were determined by broth microdilution method DPPH (1,1-diphenyl-2-picrylhydracyl) assay, respectively. Propolis samples had activity against only Gram-positive bacteria. The UP sample showed highest Staphylococcus aureus [minimal inhibitory concentration (MIC) 100 microg...
Synthetic and natural mucoadhesive biomaterials in optimized galenical formulations are potentially useful for the transmucosal delivery of active ingredients to improve their localized prolonged effects. Chitosans (CS) have potent characteristics, but exact mechanisms underpinning such interactions at molecular level role specific structural properties CS remain elusive. In present study we used a combination microviscosimetry, zeta potential analysis, isothermal titration calorimetry (ITC)...
Abstract Cancer gene therapy requires the design of non-viral vectors that carry genetic material and selectively deliver it with minimal toxicity. Non-viral based on cationic natural polymers can form electrostatic complexes negatively-charged polynucleotides such as microRNAs (miRNAs). Here we investigated physicochemical/biophysical properties chitosan–hsa-miRNA-145 (CS–miRNA) nanocomplexes biological responses MCF-7 breast cancer cells cultured in vitro . Self-assembled CS–miRNA were...
A polyelectrolyte complex system of chitosan-pectin nano- and microparticles was developed to encapsulate the hormone insulin. The aim this work obtain small particles for oral insulin delivery without chemical crosslinkers based on natural biodegradable polysaccharides. were using chitosans (with different degrees acetylation: 15.0% 28.8%) pectin solutions at various charge ratios (n⁺/n- given by chitosan/pectin mass ratio) total charge. Nano- characterized regarding particle size, zeta...
Mucoadhesive materials adhere persistently to mucosal surfaces. A mucoadhesive delivery system could therefore facilitate the controlled release of drugs and optimize their bioavailability in tissues. Polysaccharides are most versatile class natural polymers for transmucosal drug delivery. We used microviscosimetry explore mucoadhesion a library polysaccharide families with diverse structural characteristics as first step toward rational design polysaccharide-based nanoformulations. Here we...
Abstract Capsaicin has known pharmacological effects including the ability to reversibly open cellular tight junctions, among others. The aim of this study was develop a strategy enhance paracellular transport substance with low permeability (FITC-dextran) across an epithelial cell monolayer via reversible opening junctions using nanosystem comprised by capsaicin and chitosan. We compared biophysical properties free capsaicin-loaded chitosan nanocapsules, their cytotoxicity towards MDCK-C7...
This study was aimed to understand the physical processes underlying nanoprecipitation of chitosan, their influence on characteristics nanoparticles and assess capacity as drug nanocarrier.