A5080663499- Protease and Inhibitor Mechanisms
- Cardiac Fibrosis and Remodeling
- Cell Adhesion Molecules Research
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Signaling Pathways in Disease
- Cardiovascular Disease and Adiposity
- Inflammatory Bowel Disease
- Congenital heart defects research
- Power Line Communications and Noise
- MicroRNA in disease regulation
- Atherosclerosis and Cardiovascular Diseases
- Cell Image Analysis Techniques
- Wastewater Treatment and Nitrogen Removal
- Inflammatory Biomarkers in Disease Prognosis
- Radiomics and Machine Learning in Medical Imaging
- Blood Coagulation and Thrombosis Mechanisms
- Spanish Literature and Culture Studies
- Connective tissue disorders research
- Zebrafish Biomedical Research Applications
- Phagocytosis and Immune Regulation
- Cardiac Imaging and Diagnostics
- Historical and Modern Theater Studies
Centro de Investigaciones Biológicas Margarita Salas
2019-2023
Spanish National Centre for Cardiovascular Research
2012-2022
Centro de Investigaciones Energéticas, Medioambientales y Tecnológicas
2007
Consejo Superior de Investigaciones Científicas
2007
Macrophages (Mφs) produce factors that participate in cardiac repair and remodeling after myocardial infarction (MI); however, how these crosstalk with other cell types mediating is not fully understood. Here we demonstrated Mφs increased the expression of Mmp14 (MT1-MMP) 7 days post-MI. We selectively inactivated gene using a genetic strategy ( f/f : Lyz2 -Cre). This conditional KO (MAC-Mmp14 KO) resulted attenuated post-MI dysfunction, reduced fibrosis, preserved capillary network....
Sewage sludge obtained by a conventional aerobic activated process (CSS), P-rich sewage from an enhanced biological P removal (PRS), and struvite (MgNH 4PO 4 x 6H 2O) recovered anaerobic digester supernatant using low-grade MgO byproduct the calcination of natural magnesite as Mg source (STR) were evaluated sources for plant growth. For this purpose, greenhouse pot experiment was conducted P-deficient loamy sand soil perennial ryegrass ( Lolium perenne L.) test crop. The applied at rates...
Angiogenesis, the formation of new blood vessels from pre-existing ones, occurs in pathophysiological contexts such as wound healing, cancer, and chronic inflammatory disease. During sprouting angiogenesis, endothelial tip stalk cells coordinately remodel their cell-cell junctions to allow collective migration extension sprout while maintaining barrier integrity. All these processes require energy, predominant ATP generation route is glycolysis. However, it remains unclear how reaches plasma...
Matrix metalloproteinases are involved in vascular remodeling. Little is known about their immune regulatory role atherosclerosis. Here we show that mice deficient for MT4-MMP have increased adherence of macrophages to inflamed peritonea, and larger lipid deposits macrophage burden atherosclerotic plaques. We also demonstrate deficiency results higher numbers patrolling monocytes crawling adhered endothelia, the accumulation Mafb+ apoptosis inhibitor (AIM)+ at incipient lesions mice....
Heterogeneity and functional specialization among skin-resident macrophages are incompletely understood. In this study, we describe a novel subset of murine dermal perivascular that extend protrusions across the endothelial junctions in steady-state capture blood-borne macromolecules. Unlike other reconstituted by bone marrow-derived progenitors after genotoxic insult, these cells replenished an extramedullary radio-resistant UV-sensitive Bmi1+ progenitor. Furthermore, they possess...
Article3 December 2019Open Access Source DataTransparent process Endothelial MT1-MMP targeting limits intussusceptive angiogenesis and colitis via TSP1/nitric oxide axis Sergio Esteban Vascular Pathophysiology Area, Centro Nacional de Investigaciones Cardiovasculares (CNIC), Madrid, Spain Search for more papers by this author Cristina Clemente Biológicas (CIB-CSIC), Agnieszka Koziol Pilar Gonzalo Rius CIBER Enfermedades (CIBER-CV), Fernando Martínez Bioinformatics Unit, Pablo M Linares...
The microvasculature continuously adapts in response to pathophysiological conditions meet tissue demands. Quantitative assessment of the dynamic changes coronary is therefore crucial enhancing our knowledge regarding impact cardiovascular diseases perfusion and developing efficient angiotherapies. Using confocal microscopy thick sections, we developed a 3D fully automated pipeline that allows precisely reconstruct extract parameters quantify all its major features, relation smooth muscle...
Abstract Vascular smooth muscle cell (VSMC) proliferation is essential for arteriogenesis to restore blood flow after artery occlusion, but the mechanisms underlying this response remain unclear. Based on our previous findings showing increased VSMC in neonatal aorta of mice lacking protease MT4-MMP, we aimed at discovering new players process. We demonstrate that MT4-MMP absence boosted vitro PDGF-BB a cell-autonomous manner through enhanced p38 MAPK activity. Increased phospho-p38 basal...
Matrix metalloproteinases (MMPs) constitute a large group of endoproteases that play important functions during embryonic development, tumor metastasis and angiogenesis by degrading components the extracellular matrix. Within this family, we focused our study on Mt4-mmp (also called Mmp17) belongs to distinct subset is anchored cell surface via glycosylphosphatidylinositol (GPI) moiety with catalytic site exposed space. Information about its function substrates very limited date, little has...
Microvascular dysfunction and resulting tissue hypoxia is a major contributor to the pathogenesis evolution of cardiovascular diseases (CVD). Diverse gene cell therapies have been proposed preserve microvasculature or boost angiogenesis in CVD, with moderate benefit. This study tested vivo impact sequential delivery by bone-marrow (BM) cells pro-angiogenic factors vascular endothelial growth factor (VEGFA) sphingosine-1-phosphate (S1P) myocardial infarction model. For that, mouse BM were...
Introduction: Removal of poorly perfused capillaries by pruning contributes to remodeling the microvasculature optimize oxygen and nutrient delivery. Blood flow drives this process promoting intravascular migration endothelial cells in developing networks, such as yolk sac, zebrafish brain or postnatal mouse retina. Methods: In study, we have implemented innovative tools recognize capillary complex 3D coronary heart. We also experimentally tested impact decreasing on structure function...
ABSTRACT Macrophages produce factors that participate in cardiac repair and remodeling after myocardial infarction (MI); however, how these crosstalk with other cell types mediating is not fully understood. In this study, we demonstrated macrophages increased expression of Mmp14 (MT1-MMP) 7 days post-MI. Specific macrophage-targeting MT1-MMP (MT1-MMP ΔLysM mice) attenuates post-MI dysfunction, reduces fibrosis, preserves the capillary network. Mechanistically, showed activates latent TGFβ1...