A5057650956- Advanced Proteomics Techniques and Applications
- Adipose Tissue and Metabolism
- Celiac Disease Research and Management
- Aortic aneurysm repair treatments
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Osteoarthritis Treatment and Mechanisms
- Ubiquitin and proteasome pathways
- Aortic Disease and Treatment Approaches
- Adipokines, Inflammation, and Metabolic Diseases
- Monoclonal and Polyclonal Antibodies Research
- Cardiovascular Function and Risk Factors
- Toxin Mechanisms and Immunotoxins
- Cardiovascular Effects of Exercise
- Mitochondrial Function and Pathology
- Cell Adhesion Molecules Research
- Protease and Inhibitor Mechanisms
- RNA Research and Splicing
- Microscopic Colitis
- Immunotherapy and Immune Responses
- interferon and immune responses
- Fungal and yeast genetics research
- Mesenchymal stem cell research
- Glycosylation and Glycoproteins Research
- Metabolism, Diabetes, and Cancer
Centro de Investigación en Red en Enfermedades Cardiovasculares
2017-2024
Spanish National Centre for Cardiovascular Research
2015-2024
Centro de Investigación Biomédica en Red
2009-2024
Instituto de Salud Carlos III
2005-2024
Universidad Autónoma de Madrid
2005-2015
Tokyo University of Science
2015
The University of Texas Health Science Center at Houston
2015
Rutgers, The State University of New Jersey
2015
The University of Tokyo
2015
Centro de Biología Molecular Severo Ochoa
2014
ABSTRACT The Pseudomonas aeruginosa genome contains several different multidrug resistance (MDR) efflux pumps. Overproduction of these pumps reduces susceptibility to a variety antibiotics. Some recently published works have analyzed the effect overproduction MDR on bacterial virulence. Here we studied MexAB-OprM, MexCD-OprJ, MexEF-OprN, and MexXY type III secretion (T3S) in P. . system (T3SS) is used by deliver toxins directly into cytoplasm host cell. Our data indicate that overexpression...
The coordinated behavior of proteins is central to systems biology. However, the underlying mechanisms are poorly known and methods analyze coordination by conventional quantitative proteomics still lacking. We present Systems Biology Triangle (SBT), a new algorithm that allows study protein pairwise proteomics. detected statistically significant in diverse biological models very different nature subjected kinds perturbations. also revealed with unprecedented molecular detail an array...
The transcriptome of the endometrium throughout menstrual cycle has been described in recent years. However, proteomic window implantation remains unknown. aim this study was to compare proteome human pre-receptive phase versus receptive by identifying and quantifying proteins differentially expressed using differential in-gel electrophoresis (DIGE) mass spectometry (MS). Endometrial biopsies were collected at days 2 (pre-receptive) 7 (receptive) after urinary luteal hormone surge same from...
Our aim was to study the protein expression profiles of omental adipose tissue biopsies obtained from morbidly obese women with or without polycystic ovary syndrome (PCOS) at time bariatric surgery evaluate possible involvement visceral adiposity in development PCOS. Ten PCOS patients and nine control samples were included. We used two-dimensional difference gel electrophoresis (2D-DIGE) followed by in-gel digestion, mass spectrometry (MS) selected spots. The 2D-DIGE technology allowed...
Obesity is increasing exponentially in developed countries and constitutes a public health problem by enhancing the risk for metabolic disorder cardiovascular disease. Differences gene expression profiles biochemical properties have been well-described between omental subcutaneous adipose tissue humans. Because has strongly associated with development of insulin resistance, type 2 diabetes disease, we searched proteins differentially expressed these two fat depots using two-dimensional...
Objective— In the search of novel biomarkers abdominal aortic aneurysm (AAA) progression, proteins released by intraluminal thrombus (ILT) were analyzed a differential proteomic approach. Methods and Results— Different layers (luminal/abluminal) ILT AAA incubated, 2-dimensional difference in-gel electrophoresis. Several differentially expressed involved in main pathological mechanisms (proteolysis, oxidative stress, thrombosis) identified mass spectrometry. Among identified, peroxiredoxin-1...
The interferon (IFN)-stimulated gene 15 (ISG15) encodes one of the most abundant proteins induced by interferon, and its expression is associated with antiviral immunity. To identify protein components implicated in IFN ISG15 signaling, we compared proteomes ISG15-/- ISG15+/+ bone marrow derived macrophages (BMDM) after vaccinia virus (VACV) infection. results this analysis revealed that mitochondrial dysfunction oxidative phosphorylation (OXPHOS) were pathways altered BMDM treated IFN....
RBPs (RNA binding proteins) play critical roles in the cell by regulating mRNA transport, splicing, editing, and stability. The RBP SRSF3 (serine/arginine-rich splicing factor 3) is essential for blastocyst formation proper liver development function. However, its role heart has not been explored.To investigate of cardiac function.Cardiac expression was high at mid gestation decreased during late embryonic development. Mice lacking showed impaired cardiomyocyte proliferation died utero. In...
The mechanisms underlying early atherosclerotic plaque formation are not completely understood. Moreover, plasma biomarkers of subclinical atherosclerosis lacking. purpose this study was to analyze the temporal and topologically resolved protein changes taking place in human aortas with find new potential diagnostic and/or therapeutic targets. composition healthy (media layer) or atheroma (fatty streak fibrolipidic, media intima layers) analyzed by deep quantitative multiplexed proteomics....
As a global approach to gain better understanding of the mechanisms involved in pea resistance Erysiphe pisi, changes leaf proteome two genotypes differing their phenotype were analyzed by combination 2-DE and MALDI-TOF/TOF MS. Leaf proteins from control non-inoculated inoculated susceptible (Messire) resistant (JI2480) plants resolved 2-DE, with IEF 5-8 pH range SDS-PAGE on 12% gels. CBB-stained gels revealed existence quantitative qualitative differences between extracts from: (i) leaves...
Changes in the outer membrane subproteome of Escherichia coli along transition to viable but nonculturable state (VBNC) were studied. The VBNC was triggered by exposure E. cells adverse conditions such as aquatic systems, starvation, suboptimal temperature, visible light irradiation and seawater. subproteome, obtained according Molloy et al., analysed at beginning (inoculum, phase 1), after a variable time (95% population culturable, 2) when populations mainly VBNC, 3). Proteome changes...
Polymorphonuclear neutrophils (PMNs) play a main role in abdominal aortic aneurysm (AAA) progression. We have analyzed circulating PMNs isolated from AAA patients and controls by proteomic approach to identify proteins potentially involved pathogenesis.PMNs 8 (4 large >5 cm 4 small 3-5 cm) were 2D differential in-gel electrophoresis. Among differentially expressed spots, several redox balance identified mass spectrometry (eg, cyclophilin, thioredoxin reductase, catalase). Diminished catalase...
With the completion of human genome sequence, biomedical sciences have entered in "omics" era, mainly due to high-throughput genomics techniques and recent application mass spectrometry proteomics analyses. However, there is still a time lag between these technological advances their clinical setting. Our work designed build bridges high-performance routine. Protein extracts were obtained from fresh frozen normal lung non-small cell cancer samples. We applied phosphopeptide enrichment...
Rationale: Aortic dissection or rupture resulting from aneurysm causes 1% to 2% of deaths in developed countries. These disorders are associated with mutations genes that affect vascular smooth muscle cell differentiation and contractility extracellular matrix composition assembly. However, as many 75% patients a family history aortic aneurysms do not have an identified genetic syndrome. Objective: To determine the role protease MMP17/MT4-MMP arterial wall its possible relevance human...
Human mesenchymal stem cells (MSC) have been on the focus of intense clinical-oriented research due to their multilineage differentiation potential and immunomodulatory properties. However, reach clinically meaningful cell numbers for cellular therapy tissue engineering applications, MSC ex-vivo expansion is mandatory but sequential passaging results in loss proliferative, clonogenic potential. To get clues into molecular mechanisms underlying senescence resulting from extended cultivation...
Abstract Obesity is a main global health issue and an outstanding cause of morbidity mortality predisposing to type 2 diabetes (T2DM) cardiovascular diseases. Huge research efforts focused on gene expression, cellular signalling metabolism in obesity have improved our understanding these disorders; nevertheless, bridge the gap between regulation expression changes signalling/metabolism, protein levels must be assessed. We extensively analysed visceral adipose tissue from age-, T2DM-...
Human age-related diseases, including obesity and type 2 diabetes (T2DM), have long been associated to mitochondrial dysfunction; however, the role for adipose tissue mitochondria in these conditions remains unknown. We tackled impact of aging T2DM on adipocyte from obese patients by quantitating not only corresponding abundance changes proteins, but also redox alterations undergone Cys residues thereof. For that, we resorted a high-throughput proteomic approach based isobaric labeling,...
Background: Hutchinson-Gilford progeria syndrome (HGPS) is a rare disorder characterized by premature aging and death mainly because of myocardial infarction, stroke, or heart failure. The disease provoked progerin, variant lamin A expressed in most differentiated cells. Patients look healthy at birth, symptoms typically emerge the first second year life. Assessing reversibility progerin-induced damage relative contribution specific cell types critical to determining potential benefits late...
Novel algorithms based on ultratolerant database searching have paved the way for comprehensive analysis of all possible post-translational modifications (PTM) that can be detected by mass spectrometry-based proteomics, obviating their prior knowledge. These tools together with novel quantitative statistical models allow hypothesis-free approaches to study role and impact PTM biological systems. However, interpretation this information from a pathophysiological perspective is challenging due...
A cocktail sandwich ELISA based on the employ of two monoclonal antibodies (MAbs) as coating and a third MAb conjugated to horseradish peroxidase has been developed for analysis gluten in foods. Given that each displays wide specificity spectrum wheat, barley, rye oats prolamins, their combination ensures high crossreactivity with most potentially toxic gliadin, hordein, secalin avenin protein family. One unprecedented features is it permits first time barley hordeins foods, which...
Botrytis cinerea is a phytopathogenic fungi causing disease in number of important crops. It considered very complex species which different populations seem to be adapted hosts. In order characterize fungal virulence factors, proteomic research was started. A protocol for protein extraction from mycelium tissue, with separation by 2-DE and MS analysis, optimised as first approach defining the B. proteome. Around 400 spots were detected CBB-stained gels, covering 5.4-7.7 pH 14-85 kDa ranges....