A5090328708- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Microbial Metabolites in Food Biotechnology
- Gut microbiota and health
- Microbial Inactivation Methods
- Animal Behavior and Reproduction
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- Bird parasitology and diseases
- Plant and animal studies
- Bacterial Genetics and Biotechnology
- Identification and Quantification in Food
- Protein Hydrolysis and Bioactive Peptides
- Avian ecology and behavior
- Enzyme Production and Characterization
- Bacterial biofilms and quorum sensing
- Meat and Animal Product Quality
- Listeria monocytogenes in Food Safety
- Vector-borne infectious diseases
- Genomics and Phylogenetic Studies
- Insect and Pesticide Research
- Animal Nutrition and Physiology
- Aquaculture disease management and microbiota
- Insect symbiosis and bacterial influences
Universidad de Granada
2015-2024
Pfizer-University of Granada-Junta de Andalucía Centre for Genomics and Oncological Research
2019
University of Liège
1994-1998
Summary Animals live in a bacterial world, and detecting exploring adaptations favouring mutualistic relationships with antibiotic‐producing bacteria as strategy to fight pathogens are of prime importance for evolutionary ecologists. Uropygial secretion European hoopoes ( Upupa epops, Linnaeus) contains antimicrobials from that may be used prevent embryo infection. Here, we investigated the microscopic structure hoopoe eggshells looking special features adhesion antimicrobial uropygial...
The structural gene of the enterococcal peptide antibiotic AS-48 (as-48) has been identified and cloned by using two degenerate 17-mer DNA oligonucleotides on basis amino acid sequences peptides obtained digestion with Glu-C endoproteinase. That as-48 codes for a 105-amino-acid prepeptide, giving rise to 70-amino-acid mature protein. Comparative analysis demonstrated that 16-amino-acid sequence one peptides, designated V8-5, was composed 12-amino-acid corresponding C-terminal end (from...
Peptide AS-48 induces ion permeation, which is accompanied by the collapse of cytoplasmic membrane potential, in sensitive bacteria. Active transport vesicles also impaired AS-48. At low concentrations, this peptide causes permeability liposomes to low-molecular-weight compounds without a requirement for potential. Higher antibiotic concentrations induce severe disorganization, visualized under electron microscopy as aggregation and formation multilamellar structures. Electrical measurements...
1 It has been recently showed that one bacterial strain isolated from the uropygial gland of a nestling hoopoe Upupa epops produced antimicrobial peptides active against broad spectrum pathogenic bacteria. These bacteria might thus mediate properties secretions as consequence symbiotic association with hoopoes. 2 We study white (from males and non-breeding females) brown nestlings breeding hoopoes epops, well presence living inside their gland. 3 found brown, but not contained activity...
ABSTRACT The uropygial gland (preen gland) is a holocrine secretory situated at the base of tail in birds which produces hydrophobic fatty secretion. In certain birds, such as hoopoe, Upupa epops , composition this secretion influenced by both seasonal and sexual factors, becoming darker more malodorous females their nestlings during nesting phase. spread throughout plumage when bird preens itself, leaving its feathers flexible waterproof. It also thought to play role defending against...
SUMMARY Among potential agents that might damage bird feathers are certain microorganisms which secrete enzymes digest keratin, as is the case of ubiquitous bacterium Bacillus licheniformis, present in both and skin wild birds. It therefore a good candidate for testing effects defences against feather-degrading microorganisms. One these oil secreted by uropygial gland, birds use to protect their parasites. In previous studies we have shown how Enterococcus faecalis strains isolated from...
The uropygial glands of birds serve multiple functions, and there is great interspecific variability in the composition properties their secretions. A special case secretion hoopoes Upupa epops , green woodhoopoes Phoeniculus purpureus which, contrary to commonly white odourless secretions, are dark with pungent odour. Recently, bacteria have been isolated from both here we test hypothesis that responsible some secretions this group birds. We explore natural seasonal changes intersexual...
Abstract Potentially, pathogenic bacteria are one of the main infective agents against which a battery chemical and physical barriers has evolved in animals. Among these secretions by exocrine uropygial gland birds. The antimicrobial properties may prevent colonization growth microorganisms on feathers, skin eggshells. However, also favour proliferation feather mites that feed living feathers would otherwise reach eggshells during incubation if not consumed mites. Therefore, at interspecific...
ABSTRACT We report the feasibility of enterocin AS-48, a circular cationic peptide produced by Enterococcus faecalis , as new leishmanicidal agent. AS-48 is lethal to Leishmania promastigotes well axenic and intracellular amastigotes at low micromolar concentrations, with scarce cytotoxicity macrophages. induced fast bioenergetic collapse L. donovani but only partial permeation their plasma membrane limited entrance vital dyes, even concentrations beyond its full lethality. Fluoresceinated...
The in vitro antimicrobial potency of the bacteriocin AS-48 is well documented, but its clinical application requires investigation, as toxicity could be different (haemolytic and antibacterial activity blood cytotoxicity towards normal human cell lines) vivo (e.g. mice zebrafish embryos) models. Overall, results obtained are promising. They reveal negligible propensity to cause death or impede growth at therapeutic concentrations (up 27 μM) support suitability this peptide a potential agent...
The complete primary structure of the peptide antibiotic AS-48 produced by Enterococcus faecalis has been determined chemical degradation analysis. cyclic nature this 70 residues containing was demonstrated plasma desorption mass analysis generated peptides and electrospray ionisation native polypeptide. As far as we know, is first example an protein cyclised a tail-head bond formation not branching polypeptide side chains.
A region of 7.8 kb the plasmid pMB2 from Enterococcus faecalis S‐48 carrying information necessary for production and immunity peptide antibiotic AS‐48 has been cloned sequenced. It contains as‐48A structural gene plus five open reading frames ( as‐48B , as‐48C as‐48C1 as‐48D as‐48D1 ). Besides As‐48D, all predicted products are basic hydrophobic proteins with potential membrane‐spanning domains (MSDs). None them shows any homology protein sequences stored in databanks, except which...