- Probiotics and Fermented Foods
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Microbial Metabolites in Food Biotechnology
- Bacterial biofilms and quorum sensing
- Antimicrobial agents and applications
- Antimicrobial Resistance in Staphylococcus
- Meat and Animal Product Quality
- Essential Oils and Antimicrobial Activity
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Antimicrobial Peptides and Activities
- Antibiotic Resistance in Bacteria
- Biochemical and Structural Characterization
- Bacteriophages and microbial interactions
- Pharmaceutical and Antibiotic Environmental Impacts
- Microbial Metabolism and Applications
- Enzyme Production and Characterization
- Identification and Quantification in Food
- Biochemical Analysis and Sensing Techniques
- Food Safety and Hygiene
- Phytochemicals and Antioxidant Activities
- Food composition and properties
- Nanocomposite Films for Food Packaging
- Polyamine Metabolism and Applications
Universidad de Jaén
2015-2024
University of Abou Bekr Belkaïd
2019-2020
Departamento de Salud
2011
Universidad de Granada
1989-2001
Consejo Superior de Investigaciones Científicas
2000
Instituto de Estructura de la Materia
2000
University of Liège
1994
Merck & Co., Inc., Rahway, NJ, USA (United States)
1990-1991
An inhibitor of the high conductance, Ca2(+)-activated K+ channel (PK,Ca) has been purified to homogeneity from venom scorpion Buthus tamulus by a combination ion exchange and reversed-phase chromatography. This peptide, which named iberiotoxin (IbTX), is one two minor components crude blocks PK,Ca. IbTX consists single 4.3-kDa polypeptide chain, as determined polyacrylamide gel electrophoresis, analysis amino acid composition, Edman degradation. Its complete sequence defined. displays 68%...
Streptococcus faecalis S-48 produces a broad spectrum antibiotic, active against Gram-positive and Gram-negative bacteria. This substance is produced in solid liquid media also defined basal medium. It sensitive to protease, pronase, or trypsin, heating at 70 °C, alkaline pH, but resistant treatment with lipase, lysozyme, phosphatase, DNAase, RNAase, acidic neutral pHs, lower temperatures (60 °C). Several organic solvents cause precipitation, not inactivation. antibiotic has been partially...
Despite the use of several Weissella (W.) strains for biotechnological and probiotic purposes, certain species this genus were found to act as opportunistic pathogens, while W. ceti recognized be pathogenic farmed rainbow trout. Herein, we investigated potential weissellas based on in silico analyses 13 whole genome sequences available date NCBI database. Our screening allowed us find virulence determinants such collagen adhesins, aggregation substances, mucus-binding proteins, hemolysins...
Multi-drug resistant bacteria (particularly those producing extended-spectrum β-lactamases) have become a major health concern. The continued exposure to antibiotics, biocides, chemical preservatives and metals in different settings such as the food chain or environment may result development of multiple resistance co-resistance. aim present study was determine resistances (biocides, preservatives, phenolic compounds metals) bacterial isolates from seafoods. A 75.86% 87 studied were at least...
The solution structure of bacteriocin AS-48, a 70-residue cyclic polypeptide from Enterococcus faecalis, consists globular arrangement five alpha-helices enclosing compact hydrophobic core. head-to-tail union lies in the middle helix 5, fact that is shown to have pronounced effect on stability three-dimensional structure. Positive charges side chains residues 4 and turn linking 5 form cluster most probably determine its antibacterial activity by promoting pore formation cell membranes. A...
The structural gene of the enterococcal peptide antibiotic AS-48 (as-48) has been identified and cloned by using two degenerate 17-mer DNA oligonucleotides on basis amino acid sequences peptides obtained digestion with Glu-C endoproteinase. That as-48 codes for a 105-amino-acid prepeptide, giving rise to 70-amino-acid mature protein. Comparative analysis demonstrated that 16-amino-acid sequence one peptides, designated V8-5, was composed 12-amino-acid corresponding C-terminal end (from...