A5039448373- Estrogen and related hormone effects
- Hormonal and reproductive studies
- Fatty Acid Research and Health
- Immune Response and Inflammation
- NF-κB Signaling Pathways
- Prostate Cancer Treatment and Research
- Sexual Differentiation and Disorders
- Adipose Tissue and Metabolism
- Plant Reproductive Biology
- Genetic Neurodegenerative Diseases
- Hormonal Regulation and Hypertension
- Genomics, phytochemicals, and oxidative stress
- Diet, Metabolism, and Disease
- Infant Nutrition and Health
- Adrenal Hormones and Disorders
- Steroid Chemistry and Biochemistry
- Heat shock proteins research
- Plant Molecular Biology Research
- Mass Spectrometry Techniques and Applications
- Protein Tyrosine Phosphatases
- Cytokine Signaling Pathways and Interactions
- Birth, Development, and Health
- Genomics and Chromatin Dynamics
Institut de Biomedicina de la Universitat de Barcelona
2021-2024
Universitat de Barcelona
2021-2024
Mutations of the androgen receptor (AR) associated with prostate cancer and insensitivity syndrome may profoundly influence its structure, protein interaction network, binding to chromatin, resulting in altered transcription signatures drug responses. Current structural information fails explain effect pathological mutations on AR structure-function relationship. Here, we have thoroughly studied effects selected that span complete dimer interface ligand-binding domain (AR-LBD) using x-ray...
Abstract The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that controls metabolic and homeostatic processes essential for life. Although numerous crystal structures of the GR ligand-binding domain (GR-LBD) have been reported, functional oligomeric state full-length receptor, which its transcriptional activity, remains disputed. Here we present five new agonist-bound GR-LBD, along with thorough analysis previous structural work. We identify four distinct...
Glucocorticoids (GCs) exert potent antiproliferative and anti-inflammatory properties, explaining their therapeutic efficacy for skin diseases. GCs act by binding to the GC receptor (GR) mineralocorticoid (MR), co-expressed in classical non-classical targets including keratinocytes. Using knockout mice, we previously demonstrated that GR MR essential nonoverlapping functions homeostasis. These closely related receptors may homo- or heterodimerize regulate transcription, theoretically bind...
The glucocorticoid receptor (GR) is a leading drug target due to its anti-inflammatory and immunosuppressive roles. functional oligomeric conformation of full-length GR (FL-GR), which key for biological activity, remains disputed. Here we present new crystal structure agonist-bound ligand-binding domain (GR-LBD) comprising eight copies non-canonical dimer. relevance this dimer multimerization in living cells has been verified by studying single- double-point mutants FL-GR fluorescence...
SUMMARY While the cohesin complex is a key player in genome architecture, how it localizes to specific chromatin sites not understood. Recently, we and others have proposed that direct interactions with transcription factors lead localization of cohesin-loader (NIPBL/MAU2) within enhancers. Here, identify two clusters LxxLL motifs NIPBL sequence regulate dynamics, interactome, NIPBL-dependent transcriptional programs. One these interacts MAU2 necessary for maintenance NIPBL-MAU2 heterodimer....
Abstract Spinal bulbar muscular atrophy (SBMA) is caused by a polyglutamine expansion (pQe) in the N-terminal transactivation domain of human androgen receptor (AR-NTD), resulting combination toxic gain- and loss-of-function mechanisms. The structural basis these processes has not been resolved due to disordered nature NTD, which hinders experimental analyses its detailed conformations. Here, using extensive computational modelling, we show that AR-NTD forms dynamic compact regions, upon pQe...
Abstract The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that controls metabolic and homeostatic processes essential for life. Although numerous crystal structures of the GR ligand-binding domain (GR-LBD) have been reported, functional oligomeric state full-length receptor, which its transcriptional activity, remains disputed. Here we present five new agonist-bound GR-LBD, along with thorough analysis previous structural work. Biologically relevant...