A5101922249- Cancer, Hypoxia, and Metabolism
- Congenital heart defects research
- RNA modifications and cancer
- Mitochondrial Function and Pathology
- Congenital Heart Disease Studies
- Cardiovascular Function and Risk Factors
- Renal and related cancers
- Pulmonary Hypertension Research and Treatments
- Pancreatic function and diabetes
- Kawasaki Disease and Coronary Complications
- Renal cell carcinoma treatment
- Epigenetics and DNA Methylation
- Coronary Artery Anomalies
- Birth, Development, and Health
Spanish National Centre for Cardiovascular Research
2016-2025
Spanish National Cancer Research Centre
2007
BACKGROUND: Hypoxia is associated with the onset of cardiovascular diseases including cardiac hypertrophy and pulmonary hypertension. HIF2 (hypoxia-inducible factor 2) signaling in endothelium mediates arterial remodeling subsequent elevation right ventricular systolic pressure during chronic hypoxia. Thus, novel therapeutic opportunities for hypertension based on specific inhibitors have been proposed. Nevertheless, relevance beyond or adaptation to hypoxia remains elusive. Wt1 (Wilms tumor...
Clear cell renal carcinoma (ccRCC) is the most common malignant neoplasm of kidney. The majority hereditary and sporadic ccRCC cases are associated with germline somatic mutations in Von Hippel-Lindau gene (VHL), respectively. Gross deletions at VHL locus can result either or a mild clinical phenotype, absence development. Our goal this study was to identify molecular basis responsible for these differences behavior order predict patients' phenotype. Using multiplex ligation-dependent...
HIF1-alpha expression defines metabolic compartments in the developing heart, promoting glycolytic program compact myocardium and mitochondrial enrichment trabeculae. Nonetheless, its role cardiogenesis is debated. To assess importance of during heart development influence glycolysis ventricular chamber formation, herein we generated conditional knockout models Hif1a Nkx2.5 cardiac progenitors cardiomyocytes. Deletion impairs embryonic without influencing cardiomyocyte proliferation results...
Background: global deletion of Vhl leads to vascular defects and early lethality, precluding the study VHL/HIF signaling during coronary formation homeostasis. Hypoxia pathway has been associated with cardiovascular diseases involving inflammation remodeling like atherosclerosis, but its role in Kawasaki Disease (KD) remains unknown. Coronary dilatation vessel rupture are most serious complications KD, while molecular mechanisms underlying these cardiac events remain poorly understood. Here...
Cardiac function and morphology by mouse fetal echocardiography can be assessed scanning the uterus extracted from abdominal cavity (trans-uterine ultrasound) or womb (trans-abdominal ultrasound). Advantages of trans-abdominal ultrasound include (1) non-invasive longitudinal analysis at different stages, reducing animal use; (2) maintenance natural environment, diminishing perturbations on functional parameters, which are more frequent in trans-uterine conditions. Here we describe both...
Abstract Rationale Hypoxia is an important environmental cue implicated in several physiopathological processes, including heart development. Several mouse models of activation or inhibition hypoxia have been previously described. While gain function extensively characterized and indicate that HIF1 signaling needs to be tightly regulated ensure a proper cardiac development, there lack consensus the field about functional outcomes HIF1α loss. Objective In this study, we aim assess...
ABSTRACT Hypoxia is an important environmental cue in heart development. Despite of extensive characterization gain and loss function models, there disagreement about the impact HIF1α elimination cardiac tissue. Here, we used a new conditional knock out Hif1a NKX2.5 progenitors to assess morphological functional consequences developing heart. By combining histology, electron microscopy high-throughout genomics, proteomics metabolomics, found that deletion leads impaired embryonic glycolysis...