A5025299242- Kruppel-like factors research
- Neuroendocrine regulation and behavior
- Genomics, phytochemicals, and oxidative stress
- Plant Molecular Biology Research
- Receptor Mechanisms and Signaling
- RNA Research and Splicing
- Evolutionary Psychology and Human Behavior
- Genetic Syndromes and Imprinting
- Epigenetics and DNA Methylation
- Circadian rhythm and melatonin
- Genomics and Chromatin Dynamics
- Evolutionary Game Theory and Cooperation
- Thyroid Disorders and Treatments
- Reproductive System and Pregnancy
- Birth, Development, and Health
- Attachment and Relationship Dynamics
- Neurobiology and Insect Physiology Research
- Hormonal Regulation and Hypertension
- Hypothalamic control of reproductive hormones
- Animal Behavior and Reproduction
- Sex and Gender in Healthcare
- Sexual Differentiation and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- FOXO transcription factor regulation
- Neural dynamics and brain function
Stanford University
2018-2023
University of Michigan
2014-2021
Michigan United
2019
Male sexual behavior is innate and rewarding. Despite its centrality to reproduction, a molecularly specified neural circuit governing male reward remains be characterized. We have discovered developmentally wired necessary sufficient for mating. This connects chemosensory input BNSTprTac1 neurons, which innervate POATacr1 neurons that project centers regulating motor output reward. Epistasis studies demonstrate are upstream of BNSTprTac1-released substance P following mate recognition...
Cooperative, synergistic gene regulation by nuclear hormone receptors can increase sensitivity and amplify cellular responses to hormones. We investigated thyroid (TH) glucocorticoid (GC) synergy on the Krüppel-like factor 9 (Klf9) gene, which codes for a zinc finger transcription involved in development homeostasis of diverse tissues. identified regions Xenopus mouse Klf9 genes 5-6 kb upstream start sites that supported transactivation TH plus GC. Within these regions, we found an...
Summary Prairie voles are among a small group of mammals that display long-term social attachment between mating partners. Many pharmacological studies show signaling via the oxytocin receptor (OxtR) is critical for monogamy in these animals. We used CRISPR-mutagenesis to independently generate three different OxtR null mutant prairie vole lines. mutants displayed such males and females showed behavioral preference their partners over stranger opposite sex when assayed using paradigms....
Krüppel-like factor 9 (Klf9) is a zinc finger transcription that functions in neural cell differentiation, but little known about its genomic targets or mechanism of action neurons. We used the mouse hippocampus-derived neuronal line HT22 to identify genes regulated by Klf9, and we validated our findings hippocampus. engineered cells express Klf9 transgene under control tetracycline repressor, RNA sequencing modulated Klf9. found 217 repressed 21 induced also co-express biotin ligase fusion...
Abstract Thyroid hormone (TH) receptor (TR)-β (trb) is induced by TH (autoinduced) in Xenopus tadpoles during metamorphosis. We previously showed that Krüppel-like factor 9 (Klf9) rapidly the tadpole brain, associates chromatin with trb upstream region a developmental stage and TH-dependent manner, forced expression of Klf9 laevis cell line XTC-2 accelerates enhances autoinduction. Here we investigated whether can promote autoinduction brain vivo. Using electroporation-mediated gene...
An intricate transcription-translation feedback loop (TTFL) governs cellular circadian rhythms in mammals. Here, we report that the zinc finger transcription factor Krüppel-like 9 (KLF9) is regulated by this TTFL, it associates chromatin at core clock and clock-output genes, acts to modulate of gene Dbp. Our earlier genome-wide analysis mouse hippocampus-derived cell line HT22 showed KLF9 with Per1, Per3, Dbp, Tef, Bhlhe40, Bhlhe41, Nr1d1, Nr1d2. Of 3514 peaks identified cells, 1028 contain...
The hippocampus is a well-known target of thyroid hormone (TH; e.g., 3,5,3'-triiodothyronine-T3) and glucocorticoid (GC; corticosterone-CORT) action. Despite evidence that TH GC play critical roles in neural development function, few studies have identified genes patterns gene regulation influenced by the interaction these hormones at genome-wide scale. In this study we investigated T3, CORT, T3 + CORT mouse hippocampus-derived cell line HT-22. We treated cells with or for 4 hr before...
Abstract The clock protein period 1 (PER1) is a central component of the core transcription-translation feedback loop governing cell-autonomous circadian rhythms in animals. Transcription Per1 directly regulated by glucocorticoid (GC) receptor (GR), and mRNA induced stressors or injection GC. Circulating GCs may synchronize peripheral clocks with pacemaker located suprachiasmatic nucleus brain. Krüppel-like factor 9 (KLF9) zinc finger transcription that, like Per1, liganded GR, it associates...