A5045140641- Memory and Neural Mechanisms
- Single-cell and spatial transcriptomics
- Neuroscience and Neuropharmacology Research
- Neuroendocrine regulation and behavior
- Advanced Fluorescence Microscopy Techniques
- Cell Image Analysis Techniques
- Circadian rhythm and melatonin
- Neural dynamics and brain function
- RNA regulation and disease
- Congenital heart defects research
- Genomic variations and chromosomal abnormalities
- Light effects on plants
- Sleep and Wakefulness Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Cell death mechanisms and regulation
- Gene Regulatory Network Analysis
- Stress Responses and Cortisol
- Receptor Mechanisms and Signaling
- Neural and Behavioral Psychology Studies
- Plant Molecular Biology Research
- Endoplasmic Reticulum Stress and Disease
- Cardiomyopathy and Myosin Studies
- Genetics and Neurodevelopmental Disorders
Stanford University
2018-2022
Rockefeller University
2016-2019
Rationale: The cardiac conduction system (CCS) consists of distinct components including the sinoatrial node, atrioventricular His bundle, bundle branches, and Purkinje fibers. Despite an essential role for CCS in heart development function, has remained challenging to interrogate because inherent obstacles small cell numbers, large cell-type heterogeneity, complex anatomy, difficulty isolation. Single-cell RNA-sequencing allows genome-wide analysis gene expression at single-cell resolution....
The projection targets of a neuronal population are key feature its anatomical characteristics. Historically, tissue sectioning, confocal microscopy, and manual scoring specific regions interest have been used to generate coarse summaries mesoscale projectomes. We present here TrailMap, three-dimensional (3D) convolutional network for extracting axonal projections from intact cleared mouse brains imaged by light-sheet microscopy. TrailMap allows region-based quantification total axon content...
The molecular clock relies on a delayed negative feedback loop of transcriptional regulation to generate oscillating gene expression. Although the principal components are present in all circadian neurons, different neuronal clusters have varying effects rhythmic behavior, suggesting that clocks they house differently regulated. Combining biochemical and genetic techniques Drosophila, we identify phosphorylation program native master pacemaker neurons regulates timing nuclear accumulation...
Hyperexcitability of brain circuits is a common feature autism spectrum disorders (ASDs). Genetic deletion chromatin-binding protein, retinoic acid induced 1 ( RAI1 ), causes Smith–Magenis syndrome (SMS). SMS syndromic ASD associated with intellectual disability, autistic features, maladaptive behaviors, overt seizures, and abnormal electroencephalogram (EEG) patterns. The molecular neural mechanisms underlying activity in remain unclear. Here we show that panneural Rai1 deletions mice...
Studies of amnesic patients and animal models support a systems consolidation model, which posits that explicit memories formed in hippocampus are transferred to cortex over time 1–6 . Prelimbic (PL), subregion the medial prefrontal cortex, is required for expression learned fear from hours after learning until weeks later 7–12 While some studies suggested cortical neurons active during memory retrieval 13–15 , others provided evidence ongoing circuit reorganization 10,16,17 It has been...
Abstract The projection targets of a neuronal population are key feature its anatomical characterization. Historically, tissue sectioning, confocal microscopy, and manual scoring specific regions interest have been used to generate coarse summaries mesoscale projectomes. We present here TrailMap, 3D convolutional network for extracting axonal projections from intact cleared mouse brains imaged by light-sheet microscopy. TrailMap allows region-based quantification total axon content in large...