A5022817844- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cancer-related gene regulation
- Genetic factors in colorectal cancer
- Cancer Research and Treatments
- Acute Myeloid Leukemia Research
- Computational Drug Discovery Methods
- Phase-change materials and chalcogenides
- Chalcogenide Semiconductor Thin Films
- Folate and B Vitamins Research
- Conducting polymers and applications
- Genomics and Chromatin Dynamics
- Colorectal Cancer Screening and Detection
- Cancer Genomics and Diagnostics
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Gut microbiota and health
- Ferroptosis and cancer prognosis
- Acute Lymphoblastic Leukemia research
- Genetic Syndromes and Imprinting
- Spectroscopy and Laser Applications
- Medical Imaging and Pathology Studies
- Atmospheric chemistry and aerosols
- Advanced Thermoelectric Materials and Devices
- Atmospheric Ozone and Climate
Children's Nutrition Research Center at Baylor College of Medicine
2014-2025
Dalian University of Technology
2024
Baylor College of Medicine
2010-2023
The University of Texas MD Anderson Cancer Center
2004-2023
Ningbo Academy of Agricultural Sciences
2022-2023
Shenyang Pharmaceutical University
2021-2022
Jiangxi Science and Technology Normal University
2018-2020
Hefei University of Technology
2019
Chinese Academy of Sciences
2015-2018
Jilin University
2015-2018
Abstract BACKGROUND Aberrant DNA methylation, which results in leukemogenesis, is frequent patients with myelodysplastic syndromes (MDS) and a potential target for pharmacologic therapy. Decitabine indirectly depletes methylcytosine causes hypomethylation of gene promoters. METHODS A total 170 MDS were randomized to receive either decitabine at dose 15 mg/m 2 given intravenously over 3 hours every 8 days (at 135 per course) repeated 6 weeks, or best supportive care. Response was assessed...
Colon cancer has been viewed as the result of progressive accumulation genetic and epigenetic abnormalities. However, this view does not fully reflect molecular heterogeneity disease. We have analyzed both (mutations BRAF, KRAS, p53 microsatellite instability) alterations (DNA methylation 27 CpG island promoter regions) in 97 primary colorectal patients. Two clustering analyses on basis either profiling or a combination were performed to identify subclasses with distinct signatures....
Aberrant methylation of promoter CpG islands in cancer is associated with silencing tumor-suppressor genes, and age-dependent hypermethylation normal appearing mucosa may be a risk factor for human colon cancer. It not known whether this age-related DNA phenomenon specific to tissues. We performed comprehensive profiling regions aging mouse intestine using methylated island amplification combination microarray analysis. By comparing C57BL/6 mice at 3-mo-old versus 35-mo-old 3627 detectable...
Sporadic colorectal cancers often arise from a region of cells characterized by "field defect" that has not been well defined molecularly. DNA methylation proposed as candidate mediator this field defect. The repair gene O6-methylguanine-DNA methyltransferase (MGMT) is frequently methylated in cancer. We hypothesized MGMT could be one the mediators cancerization colon mucosa.We studied promoter three different bisulfite-based techniques tumor, adjacent mucosa, and non-adjacent mucosa 95...
Throughout most of the mammalian genome, genetically regulated developmental programming establishes diverse yet predictable epigenetic states across differentiated cells and tissues. At metastable epialleles (MEs), conversely, epigenotype is established stochastically in early embryo then maintained lineages, resulting dramatic systemic interindividual variation regulation. In mouse, maternal nutrition affects this process, with permanent phenotypic consequences for offspring. MEs have not...
The role of CpG island methylation in normal development and cell differentiation is keen interest, but remains poorly understood. We performed comprehensive DNA profiling promoter regions peripheral blood by methylated amplification combination with microarrays. This technique allowed us to simultaneously determine the status 6,177 genes, 92% which include dense islands. Among these 5,549 autosomal genes promoters, we have identified 4.0% that are nearly completely blood, providing another...
Purpose The current classification systems of myelodysplastic syndromes (MDS), including the International Prognostic Scoring System (IPSS), do not fully reflect molecular heterogeneity disease. Molecular characterization may predict clinical outcome and help stratify patients for targeted therapies. Epigenetic therapy using decitabine, a DNA hypomethylating agent, is clinically effective treatment MDS. Therefore, we investigated association between methylation in Patients Methods We...
The mechanism of DNA hypermethylation-associated tumor suppressor gene silencing in cancer remains incompletely understood. Here, we show by chromatin immunoprecipitation that for three genes (P16, MLH1, and the O(6)-methylguanine-DNA methyltransferase gene, MGMT), histone H3 Lys-9 methylation directly correlates acetylation inversely with neoplastic cell lines. Treatment deacetylase inhibitor trichostatin A (TSA) resulted moderately increased at silenced loci no effect on minimal effects...
Abstract 5-Aza-2′-deoxycytidine (decitabine) is postulated to have clinical activity in myeloid leukemias via its ability inhibit DNA methylation. To study this, we examined methylation patients with leukemia treated decitabine. Five days after the treatment, total genomic 5-methylcytosine/cytosine decreased on average by 14% (from 4.3% 3.7%), whereas of repetitive elements showed a mean decrease 9% and 16% for Alu long interspersed nucleotide elements, respectively. Methylation linearly...
Abstract Our laboratory has shown that vascular endothelial growth factor receptor-1 (VEGFR-1) expression on human pancreatic cancer cell lines mediates migration and invasion. Because epithelial to mesenchymal transition (EMT) also plays a role in motility by altering the phenotype morphology, we hypothesized VEGFR-1 activation induces molecular alterations mediate EMT. treatment of line L3.6pl with ligands VEGF-A VEGF-B led morphologic changes characteristic EMT, including loss polarity,...
BackgroundAlterations in DNA methylation cancer include global hypomethylation and gene-specific hypermethylation. It is not clear whether these two epigenetic errors are mechanistically linked or occur independently. This study was performed to determine the relationship between hypomethylation, hypermethylation microsatellite instability cancer.Methodology/Principal FindingsWe examined 61 cell lines 60 colorectal carcinomas their adjacent tissues using LINE-1 bisulfite-PCR as a surrogate...
Background: Hypermethylation of a CpG-rich promoter (CpG island) blocks expression the corresponding gene. The CpG island methylator phenotype (CIMP), defined as variable pattern hypermethylation islands in tumor suppressor genes, may be associated with carcinogenesis. To determine whether CIMP is development hepatocellular carcinoma (HCC) and exposure to environmental agents, we examined methylation status HCCs from countries various HCC risks. Methods: We 12 (eight for known genes) 85...
Modifications of the histone amino-terminal tails affect access regulatory factors and complexes to chromatin thereby influence biological processes. Cancer cells are characterized by prominent epigenetic dysregulation, including modifications. However, functional roles methyltransferases (HMT) in cancer remain unclear.We studied RNAi-based inhibition (knockdown, KD) 2 different H3K9 HMTs, SUV39H1 G9a. Knockdown HMTs PC3 cell line markedly inhibited growth caused profound morphological...
Abstract Purpose: Prostate cancer is a leading cause of death among the aging male population but mechanism underlying this association unclear. Aberrant methylation promoter CpG islands associated with silencing genes and age-dependent several has been proposed as risk factor for sporadic cancer. We examined extent gene in pathologically normal human prostate function age. Experimental Design: used pyrosequencing to quantitatively analyze status nine tissue DNA from 45 organ donors patients...
The aim of the present study was to examine DNA methylation and histone modification changes in hepatocellular carcinomas (HCC).DNA P16, RASSF1a, progesterone receptor (PGR) estrogen alpha (ERalpha) promoters determined by quantitative bisulfite-pyrosequencing technique HCC patients. Histone H3-lysine (K) 4, H3-K9 H3-K27 modifications all these four genes were examined chromatin immunoprecipitation (ChIP) assay cell lines. Expression two methyltransferases (DNMT1 DNMT3b) three (SUV39H1, G9a...
Abstract Purpose: There are no good genomic markers of survival in patients with advanced colorectal cancer. The CpG island methylator phenotype (CIMP) marks a distinctive pathway We sought to determine the prognostic significance CIMP cancer treated 5-fluorouracil (5-FU) an Eastern Cooperative Oncology Group clinical trial. Experimental Design: studied 188 enrolled on protocol E2290, five-arm trial comparing 5-FU, 5-FU combination N-phosphonoacetyl-l-aspartic acid, oral leucovorin, i.v. or...
Studies of embryonic stem cells (ESCs) reveal that these cell lines can be derived from differing stages development. We analyzed common changes in the expression microRNAs (miRNAs) and mRNAs 9 different human ESC (hESC) during early commitment further examined key ESCenriched miRNAs earlier developmental states several species. show previously defined hESC-enriched miRNA groups (the miR-302, -17, -515 families, miR-371-373 cluster) other are down-regulated rapidly response to...
An abnormal pattern of DNA methylation occurs at specific genes in almost all neoplasms. The lack high-throughput methods with high specificity and sensitivity to detect changes has limited its application for clinical profiling. Here we overcome this limitation present an improved method identify methylated genome-wide by hybridizing a CpG island microarray amplicons obtained the amplification technique (MCAM). We validated three cancer cell lines 15 primary colorectal tumors, resulting...
Abstract Introduction Aberrant DNA methylation has been found frequently in human breast cancers, associated with the loss of expression a number regulatory genes for growth and correlated to clinical outcomes. The present study was undertaken determine whether set growth-suppressor would correlate estrogen receptors (ERs) progesterone (PRs). Methods We used pyrosequencing analysis 12 known 90 pairs malignant/normal tissues. also examined ERs PRs those specimens by immunohistochemistry....
Abstract Background: Global loss of methylated cytosines in DNA, thought to predispose chromosomal instability and aneuploidy, has been associated with an increased risk colorectal neoplasia. Little is known about the relationships between global hypomethylation lifestyle, demographics, dietary measures, genetic factors. Methods: Our data were collected as part a randomized clinical trial testing efficacy aspirin folic acid for prevention adenomas. At surveillance colonoscopy ∼3 years after...