A5006728046- Inflammatory Bowel Disease
- Microscopic Colitis
- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Gastrointestinal motility and disorders
- Immunodeficiency and Autoimmune Disorders
- Helicobacter pylori-related gastroenterology studies
- Epigenetics and DNA Methylation
- Eosinophilic Esophagitis
- Celiac Disease Research and Management
- Genetic and rare skin diseases.
- Digestive system and related health
- IL-33, ST2, and ILC Pathways
- Adolescent and Pediatric Healthcare
- Pediatric Hepatobiliary Diseases and Treatments
- Autoimmune and Inflammatory Disorders
- Infant Nutrition and Health
- Gastrointestinal disorders and treatments
- Immune responses and vaccinations
- Hedgehog Signaling Pathway Studies
- Cancer and Skin Lesions
- Autoimmune and Inflammatory Disorders Research
- Liver Diseases and Immunity
- Viral gastroenteritis research and epidemiology
- Genetic Syndromes and Imprinting
Children's Nutrition Research Center at Baylor College of Medicine
2016-2025
Baylor College of Medicine
2016-2025
Texas Children's Hospital
2016-2025
Arkansas Children's Nutrition Center
2010-2024
Agricultural Research Service
2023-2024
United States Department of Agriculture
2018-2024
Primary Children's Hospital
2023
University of Utah
2023
Emory University
2018
Vanderbilt University Medical Center
2018
Throughout most of the mammalian genome, genetically regulated developmental programming establishes diverse yet predictable epigenetic states across differentiated cells and tissues. At metastable epialleles (MEs), conversely, epigenotype is established stochastically in early embryo then maintained lineages, resulting dramatic systemic interindividual variation regulation. In mouse, maternal nutrition affects this process, with permanent phenotypic consequences for offspring. MEs have not...
Developmental epigenetic changes, such as DNA methylation, have been recognized potential pathogenic factors in inflammatory bowel diseases, the hallmark of which is an exaggerated immune response against luminal microbes. A methyl-donor (MD) diet can modify methylation at select murine genomic loci during early development. The components MDs are routinely incorporated into prenatal human supplements. Therefore, we studied effects maternal MD supplementation on offspring colitis...
The connection between intestinal micro-biota and host physiology is increasingly becoming recognized. details of this dynamic interaction, however, remain to be explored. Toll-like receptor 2 (Tlr2) important for its role in bacterial recognition, inflammation, obesity-related metabolic changes. Therefore, we sought determine the epigenomic metagenomic consequences Tlr2 deficiency colonic mucosa mice gain insights into biological pathways that shape interface gut mammalian host. Colonic...
Inflammatory bowel diseases (IBDs) are heterogeneous disorders with complex aetiology. Quantitative genetic studies suggest that only a small proportion of the disease variance observed in IBD is accounted for by variation, indicating potential role differential epigenetic regulation The aim this study was to assess genome-wide DNA methylation changes specifically associated ulcerative colitis (UC), Crohn's (CD) and activity.DNA quantified peripheral blood mononuclear cells (PBMCs) from 149...
ABSTRACT Fecal microbiota transplantation (FMT) is becoming part of the treatment algorithms against recurrent Clostridium difficile infection (rCDI) both in adult and pediatric gastroenterology practice. With our increasing recognition critical role microbiome plays human health disease, FMT also being considered as a potential therapy for other disorders, including inflammatory bowel disease (Crohn ulcerative colitis), graft versus host neuropsychiatric diseases, metabolic syndrome....
ABSTRACT Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from single healthy human donor to three pediatric ulcerative colitis patients, each whom received course 22 30 treatments. Viral particles were purified and recipient stool samples sequenced; the reads then assembled into contigs corresponding viral...
Inflammatory bowel diseases (IBD) are chronic intestinal inflammatory disorders characterized by a complex disruption of the physiologic interaction between host immune system and microbes precipitated environmental factors. Numerous observations indicate altered composition function microbiome patients with ulcerative colitis (UC), subtype IBD. The accuracy these results may be limited confounding factors, such as concurrent medication use. To address limitations, we examined colonic...
Background and aims: Bacteriotherapy aimed at addressing dysbiosis may be therapeutic for Inflammatory Bowel Diseases (IBDs). We sought to determine if defined Bacteroides-based bacteriotherapy could an effective consistent alternative fecal microbiota transplantation (FMT) in a murine model of IBD. Methods: induced experimental colitis 8- 12-week-old C57BL/6 mice using 2-3% dextran sodium sulfate. Mice were simultaneously treated by oral gavage with triple-Bacteroides cocktail, individual...
It is well established that the vacuole plays an important role in cellular adaptation to growth presence of elevated extracellular Ca 2+ concentrations Saccharomyces cerevisiae . The ATPase Pmc1p and /H + exchanger Vcx1p/Hum1p have been shown facilitate sequestration into vacuole. However, distinct physiological roles these two vacuolar transporters remain uncertain. Here we show Vcx1p can rapidly sequester a sudden pulse cytosolic vacuole, while carries out this function much less...
Crohn's disease (CD) and ulcerative colitis (UC) are common forms of inflammatory bowel (IBD). Monozygotic (MZ) twin discordance rates epidemiologic data implicate that environmental changes epigenetic factors may play a pathogenic role in IBD. DNA methylation (the cytosines within CpG dinucleotides) is an modification, which can respond to influences. We investigated whether might be connected with IBD peripheral blood leukocyte (PBL) by utilizing genome-wide microarrays.Two different...
Inflammatory bowel diseases (IBD) are emerging globally, indicating that environmental factors may be important in their pathogenesis. Colonic mucosal epigenetic changes, such as DNA methylation, can occur response to the environment and have been implicated IBD pathology. However, methylation has not examined treatment-naïve patients. We studied untreated, left sided colonic biopsy specimens using Infinium HumanMethylation450 BeadChip array. analyzed 22 control (C) patients, 15 untreated...
Brooke–Spiegler syndrome (BSS), familial cylindromatosis (FC), and multiple trichoepithelioma (MFT), originally described as distinct inherited disorders, are characterized by a variety of skin appendage neoplasms. Mutations in the CYLD gene found individuals with these syndromes. We describe single family affected members exhibiting either FC or MFT phenotypes associated mutation gene. These findings support notion that BSS, FC, represent phenotypic variation defect. Of interest, one this...
Metastable epialleles (MEs) are mammalian genomic loci where epigenetic patterning occurs before gastrulation in a stochastic fashion leading to systematic interindividual variation within one species. Importantly, periconceptual nutritional influences may modulate the establishment of changes, such as DNA methylation at MEs. Based on these characteristics, we exploited Infinium HumanMethylation450 BeadChip kits 2-tissue parallel screen peripheral blood leukocyte and colonic mucosal from 10...