A5071741783- Animal Genetics and Reproduction
- HIV Research and Treatment
- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- HIV/AIDS drug development and treatment
- Plant Virus Research Studies
- Cancer therapeutics and mechanisms
- Pancreatic function and diabetes
- Biochemical and Molecular Research
- Cannabis and Cannabinoid Research
- Renal and related cancers
- Cell Adhesion Molecules Research
- NF-κB Signaling Pathways
- Cellular Mechanics and Interactions
- Natural product bioactivities and synthesis
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Protease and Inhibitor Mechanisms
- Biosensors and Analytical Detection
- Polyomavirus and related diseases
- Phytochemistry and Bioactive Compounds
- Bone health and treatments
- Phytochemical Studies and Bioactivities
Eulji University
2015-2025
Ewha Womans University
2025
University of Pennsylvania
2014-2024
California University of Pennsylvania
2024
University of California, Los Angeles
2023
Philadelphia University
2017-2022
Center for Cancer Research
2022
California Institute for Regenerative Medicine
2020-2022
Harvard University
2022
Cornell University
2022
Six distinct peroxiredoxin (Prx) proteins (Prx I-VI) from genes have been identified in mammalian tissues. Prxs are members of a group peroxidases that conserved reactive cysteine residue(s) the active site(s). An immediate physiological electron donor for peroxidase catalysis five Prx I-V) has as thioredoxin (Trx), but VI (1-Cys Prx) is still unclear. To identify an and binding protein VI, we performed overlay assay. A 20-kDa was by assay with flow-through fractions High-Q column rat lung...
Whole-genome expression profiling in postmortem brain tissue has recently provided insight into the pathophysiology of schizophrenia. Previous microarray and RNA-Seq studies identified several biological processes including synaptic function, mitochondrial function immune/inflammation response as altered cortex subjects with Now using data from hippocampus, we have 144 differentially expressed genes schizophrenia cases compared unaffected controls. Immune/inflammation was main process...
The genomic DNAs of tailed bacteriophages are commonly modified by the attachment chemical groups. Some forms DNA modification known to protect phage from cleavage restriction enzymes, but others unknown function. Recently, CRISPR-Cas nuclease complexes were shown mediate bacterial adaptive immunity RNA-guided target recognition, raising question whether modifications may also block attack CRISPR-Cas9. We investigated T4 as a model system, where cytosine is replaced with...
Abstract Establishment of spermatogonia throughout the fetal and postnatal period is essential for production spermatozoa male fertility. Here, we establish a protocol in vitro reconstitution human prospermatogonial specification whereby primordial germ cell (PGC)-like cells differentiated from induced pluripotent stem are further into M-prospermatogonia-like T1 prospermatogonia-like (T1LCs) using long-term cultured xenogeneic reconstituted testes. Single RNA-sequencing used to delineate...
ABSTRACT Fecal microbiota transplantation (FMT) is a highly effective treatment for refractory Clostridium difficile infections. However, concerns persist about unwanted cotransfer of pathogenic microbes such as viruses. Here we studed FMT from single healthy human donor to three pediatric ulcerative colitis patients, each whom received course 22 30 treatments. Viral particles were purified and recipient stool samples sequenced; the reads then assembled into contigs corresponding viral...
Abstract Background Rapid spread of SARS-CoV-2 has led to a global pandemic, resulting in the need for rapid assays allow diagnosis and prevention transmission. Reverse transcription-polymerase chain reaction (RT-PCR) provides gold standard assay RNA, but instrument costs are high supply chains potentially fragile, motivating interest additional methods. transcription loop-mediated isothermal amplification (RT-LAMP) an alternative that uses orthogonal often less expensive reagents without...
MicroRNAs (miRNAs) play a critical role in determining the differentiation fate of pluripotent stem cells and germ mammals. However, mechanism(s) miRNA-mediated posttranscriptional regulation with regard to lineage specification chick development require further investigation. Therefore, we conducted miRNA expression profiling explore specific signatures undifferentiated blastoderm primordial (PGCs). We identified seven miRNAs that are highly expressed 10 PGCs. In this study, miR-302a...
Rationale: The etiology of sarcoidosis is unknown, but microbial agents are suspected as triggers.Objectives: We sought to identify bacterial, fungal, or viral lineages in specimens from patients with enriched relative control subjects using metagenomic DNA sequencing. Because environmental contamination contributes disproportionately samples low authentic content, we developed improved methods for filtering contamination.Methods: analyzed (n = 93), without 72), and various controls 150)....
The allosteric inhibitors of integrase (termed ALLINIs) interfere with HIV replication by binding to the viral-encoded (IN) protein. Surprisingly, ALLINIs not DNA integration but viral particle assembly late during replication. To investigate ALLINI inhibitory mechanism, we crystallized full-length HIV-1 IN bound GSK1264 and determined structure complex at 4.4 Å resolution. shows buried between C-terminal domain (CTD) catalytic core domain. In crystal lattice, interacting domains are...
At present no antiviral agents are available for treatment of infection by the pathogenic poxvirus molluscum contagiosum virus (MCV). Here we report identification and characterization an inhibitor active against virus-encoded type-1 topoisomerase, enzyme likely to be required MCV replication. We screened a library marine extracts natural products from microorganisms using topoisomerase assays in vitro. The cyclic depsipeptide sansalvamide A was found inhibit topoisomerase-catalyzed DNA...
Induction of matrix metalloproteinase (MMP)-9 is particularly important for the invasiveness breast cancers. We investigated inhibitory effect kalopanaxsaponin A (KPS-A) on cell invasion and MMP-9 activation in phorbol 12-myristate 13-acetate (PMA)-treated MCF-7 human cancer cells. KPS-A inhibited PMA-induced proliferation invasion. was blocked presence a primary antibody MMP-9, suppressed increased expression and/or secretion tissue inhibitor (TIMP)-1. Using specific inhibitors, we...
Abstract The choroid plexus (CP) has a key role in maintaining brain homeostasis by producing cerebrospinal fluid (CSF), mediating transport of nutrients and removing metabolic products from the central nervous system responding to peripheral inflammatory signals. Although abnormal markers immune response inflammation are apparent individuals with schizophrenia, CP these not been characterized. We therefore sequenced mRNA two independent collections schizophrenia unaffected controls. Genes...
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) have facilitated the production of genome-edited animals for use as models. Because their unique developmental system, avian species offer many advantages model vertebrates. Here, we report development novel chicken models using CRISPR/Cas9-mediated nonhomologous end joining repair pathway in primordial germ cells (PGCs). Through introduction a donor plasmid containing guide RNA...
Melanogenesis is the biological process which skin pigment melanin synthesized to protect against ultraviolet irradiation and other external stresses. Abnormal biology of melanocytes closely associated with depigmented disorders such as vitiligo. In this study, we examined effects maclurin on melanogenesis cytoprotection. Maclurin enhanced cellular tyrosinase activity well levels. We found that treatment increased expression microphthalmia-associated transcription factor (MITF),...
Development of the adrenal cortex, a vital endocrine organ, originates in adrenogonadal primordium, common progenitor for both adrenocortical and gonadal lineages rodents. In contrast, we find that humans cynomolgus monkeys, lineage temporally spatially distinct fashion from lineage, arising earlier more anteriorly within coelomic epithelium. The primordium arises adrenogenic epithelium via an epithelial-to-mesenchymal transition, which then progresses into steroidogenic fetal zone direct...