A5025239705- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- HIV Research and Treatment
- Microbial infections and disease research
- Clostridium difficile and Clostridium perfringens research
- Rabies epidemiology and control
- Virology and Viral Diseases
- Vector-borne infectious diseases
- Microscopic Colitis
- Wildlife Ecology and Conservation
- Turtle Biology and Conservation
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Immunology Research
- Gastrointestinal motility and disorders
- Gut microbiota and health
- T-cell and Retrovirus Studies
- Infection Control and Ventilation
- Helicobacter pylori-related gastroenterology studies
- Molecular Biology Techniques and Applications
International Rett syndrome Foundation
2024
Rome Foundation
2024
University of Tennessee at Knoxville
2010-2023
Center for Veterinary Medicine
2023
University of Leeds
2023
Children's Hospital of Philadelphia
2018-2021
Knoxville College
1994-2020
University of Sadat City
2014
United States Army
2014
University of Tennessee System
2012
A highly fatal hemorrhagic disease has been identified in 10 young Asian and African elephants at North American zoos. In the affected animals there was ultrastructural evidence for herpesvirus-like particles endothelial cells of heart, liver, tongue. Consensus primer polymerase chain reaction combined with sequencing yielded molecular that confirmed presence two novel but related herpesviruses associated disease, one another elephants. Otherwise healthy external herpetic lesions herpesvirus...
Rett syndrome (RTT) is rare neurodevelopmental disorder caused by mutations in the
The physiological decline that occurs in aging is thought to result, part, from accumulation of oxidative damage generated by reactive oxygen species during normal metabolic processes. Elevated levels antioxidative enzymes liver tissues are present the Ames dwarf, a growth hormone (GH)-deficient mouse lives more than 1 year longer wild-type mice same line. In contrast, transgenic overexpress GH exhibit depressed hepatic catalase and have significantly shortened life spans. this study, we...
Ranaviruses (genus Ranavirus) have been observed in disease epidemics and mass mortality events free-ranging amphibian, turtle, tortoise populations worldwide. Infection is highly fatal turtles, the potential impact on endangered could be devastating. Our objectives were to determine prevalence of ranavirus DNA blood oral swabs, report associated clinical signs infection, spatial distribution infected turtles. Blood swabs taken from 140 eastern box turtles (Terrapene carolina carolina) that...
Abstract Cellular differentiation is directly determined by concentration gradients of morphogens. As a central model for gradient formation during development, Hedgehog (Hh) morphogens spread away from their source to direct growth and pattern in Drosophila wing eye discs. What not known how extracellular Hh achieved it translates into precise gradients. Here we show that two separate binding areas located on opposite sides the molecule can interact simultaneously with heparan sulfate (HS)...
Kidney, heart, liver, and, to a lesser extent, pancreas and lung transplants have become routine treatments for end‐stage organ failure. The short‐term success rate is high. Graft loss from allograft rejection, the traditional enemy of transplantation, has been greatly reduced. These improved results yielded patient survival rates at 1 3 years that exceed 90%.
Virucidal efficacy was evaluated for four recently available disinfectants: chlorine dioxide, potassium peroxymonosulfate, a quaternary ammonium compound, and citricidal (grapefruit extract). Sodium hypochlorite (3%) tap water were used as positive negative controls respectively. Feline herpesvirus, feline calicivirus, parvovirus exposed to the manufacturers’ recommended dilutions of disinfectants. Both dioxide peroxymonosulfate completely inactivated three viruses in this study. These...
Canine distemper virus (CDV) remains a common cause of infectious disease in dogs, particularly high-density housing situations such as shelters. Vaccination all dogs against CDV is recommended at the time admission to animal shelters and many use modified live (MLV) vaccine. From diagnostic standpoint for with suspected infection, this problematic because highly sensitive real-time reverse transcription polymerase chain reaction (RT-PCR) tests are able detect MLV clinical samples. Real-time...
In late 2019, a novel coronavirus began circulating within humans in central China. It was designated SARS-CoV-2 because of its genetic similarities to the 2003 SARS (SARS-CoV). Now that has spread worldwide, there is risk it establishing new animal reservoirs and recombination with native coronaviruses. To screen local populations United States for exposure SARS-like coronaviruses, we developed serological assay using receptor binding domain (RBD) from SARS-CoV-2. SARS-CoV-2's RBD...
The virucidal activity of several disinfectants containing newer generation quaternary ammonium compounds (QACs) as their active ingredients was evaluated. Disinfectants were used at the manufacturers' recommended dilutions with isolates feline herpesvirus, calicivirus, and canine parvovirus, a contact time 10 minutes room temperature. Detoxification virus/disinfectant solutions done by dialysis prior to virus assay in cell cultures. Two four completely inactivated two significantly reduced...
Abstract Objective —To measure antibody titers against bovine coronavirus (BCV), determine frequency of BCV in nasal swab specimens, and compare calves treated for respiratory tract disease (BRD) between those given an intranasally administered vaccine control calves. Design —Randomized clinical trial. Animals —414 heifer Procedure —Intranasal antigen concentration titer were measured on entry to a feedlot. Calves randomly assigned receive 3.0 mL modified-live virus enteric rotavirus or...
Feline calicivirus (FCV) is 1 of the most common causes upper respiratory tract disease in cats. Other syndromes associated with FCV infection have been reported. Recently, a hemorrhagic-like leading to significant mortality cats has The clinical signs are similar those observed rabbit hemorrhagic disease. This study characterized 2 isolates Nucleotide sequencing complete genome done for these as well 4 additional representing other syndromes. Previously reported sequence data entire...
Children with inflammatory bowel disease [IBD] are disproportionally affected by recurrent Clostridioides difficile infection [rCDI]. Although faecal microbiota transplantation [FMT] has been used good efficacy in adults IBD, little is known about outcomes associated FMT paediatric IBD.We performed a retrospective review of at 20 centres the USA from March 2012 to 2020. and without IBD were compared determined differences for rCDI. In addition, children successful outcome predictors success....
Feline coronavirus (FCoV) is an important pathogen of domestic and nondomestic Felidae. Investigation into the prevalence FCoV in exotic Felidae has relied primarily on serology. The usefulness genetic detection using reverse transcription nested polymerase chain reaction (RT/nPCR) for viral screening was investigated. Seventy-five biologic samples, feces, from captive felids 11 institutions were tested PCR. Serum samples collected all but 12 these animals antibodies to type I II by indirect...
Blood samples were analyzed from 30 domestic cats (Felis domesticus) the Petén region of Guatemala to determine seroprevalence common pathogens that may pose a potential risk native wild felids. Eight had been vaccinated previously; however, owners unable fully describe type vaccine and date administration. In addition, blood obtained two captive margays (Leopardus wiedii). Samples tested for antibodies feline immunodeficiency virus, Dirofilaria immitis, panleukopenia herpesvirus,...
Abstract A colony of domestic rabbits in Tennessee, USA, experienced a high-mortality (~90%) outbreak enterocolitis. The clinical characteristics were one to six days lethargy, bloating, and diarrhea, followed by death. Heavy intestinal coccidial load was consistent finding as mucoid enteropathy with cecal impaction. Preliminary analysis electron microscopy revealed the presence virus-like particles stool affected rabbits. Analysis using Virochip, viral detection microarray, suggested an...