A5001228618- Antimicrobial Resistance in Staphylococcus
- Microbial infections and disease research
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Virus-based gene therapy research
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Viral gastroenteritis research and epidemiology
- Veterinary Oncology Research
- Dermatological diseases and infestations
- Vector-borne infectious diseases
- Infectious Diseases and Mycology
- HIV Research and Treatment
- Respiratory viral infections research
- SARS-CoV-2 and COVID-19 Research
- Cervical Cancer and HPV Research
- Biochemical and Structural Characterization
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Helminth infection and control
- Antimicrobial Peptides and Activities
- Infective Endocarditis Diagnosis and Management
- Nail Diseases and Treatments
- Dermatology and Skin Diseases
University of Tennessee at Knoxville
2015-2024
Knoxville College
2013-2024
Medical Diagnostic Laboratories (United States)
2019
University of San Diego
2017
University of Bern
2016
University of Sadat City
2014
Hendrix College
2013
Colorado State University
2013
Tufts University
2013
IDEXX Laboratories (United States)
2011
The aim of this study was to determine the phenotypic and genotypic resistance profiles methicillin-resistant Staphylococcus pseudintermedius (MRSP) examine clonal distribution in Europe North America. A total 103 MRSP isolates from dogs isolated several countries Europe, USA Canada were characterized. Isolates identified by PCR–restriction fragment length polymorphism (RFLP), antimicrobial susceptibility determined broth dilution or gradient diffusion, genes detected using a microarray....
Abstract Objective —To determine whether resistance to oxacillin and other antimicrobials in 3 Staphylococcus spp commonly isolated from dogs increased 2001 2005. Design —Retrospective case series. Sample Population —1,772 clinical samples of various types obtained examined at the University Tennessee Veterinary Teaching Hospital or regional veterinary hospitals submitted bacteriology mycology laboratories associated with teaching hospital. Procedures —Samples were by attending veterinarians...
ABSTRACT Staphylococcus pseudintermedius is an opportunistic pathogen in dogs. Four housekeeping genes with allelic polymorphisms were identified and used to develop expanded multilocus sequence typing (MLST) scheme. The new seven-locus technique shows S. have greater genetic diversity than previous methods discriminates more isolates based upon host origin.
The Hurler syndrome (alpha-L-iduronidase deficiency disease) is a severe lysosomal storage disorder that potentially amenable to enzyme-replacement therapy. Availability of canine model the disease and sufficient supply corrective enzyme have permitted therapeutic trial lasting 3 mo. Recombinant human alpha-L-iduronidase, purified apparent homogeneity from secretions stably transfected Chinese hamster ovary cell line, was administered i.v. homozygous affected animals in doses approximately 1...
Clinical and Laboratory Standards Institute interpretive breakpoints for in vitro susceptibility tests that predict mecA-mediated oxacillin resistance Staphylococcus pseudintermedius isolates from animals have been changed twice the past decade. Moreover, there are no counterpart recommendations human of S. pseudintermedius. Individual medical veterinary laboratories variably use identical to those recommended with aureus or coagulase-negative staphylococci. The purpose current study was...
This study evaluated the exposure of dogs to three different Ehrlichia spp. in south and central regions United States where vector-borne disease prevalence has been previously difficult ascertain, particularly beyond metropolitan areas.
Multidrug- and methicillin-resistant staphylococci are both veterinary public health concerns due to their zoonotic potential. Therefore, the objective of this study was investigate patterns antimicrobial, multidrug, methicillin resistance among four Staphylococcus spp. commonly isolated from canine clinical specimens submitted Clinical Bacteriology Laboratory at University Tennessee College Veterinary Medicine (UTCVM).Results antimicrobial susceptibility testing mecA polymerase chain...
Abstract Objective —To determine the methicillin-resistant profile of staphylococcal isolates from skin dogs with pyoderma. Animals —90 Procedure —Staphylococci isolated pyoderma were tested for susceptibility to methicillin by use a standard disk diffusion test oxacillin disks. The DNA extracted was mecA gene that encodes penicillinbinding protein 2a (PBP2a) polymerase chain reaction (PCR) assay. expression PBP2a determined commercial latex agglutination Species identified morphologic,...
Abstract Objective —To determine frequency with which Staphylococcus schleiferi could be isolated from dogs pyoderma and antimicrobial susceptibility patterns of isolates that were obtained. Design —Prospective study. Animals —54 a first (n = 14) or recurrent (40) episode pyoderma. Procedure —Specimens obtained submitted for bacterial culture. Isolates identified as S on the basis growth biochemical characteristics. Two DNA sequencing to confirm identification. Methicillin was determined by...
Journal Article Cross-Reactivity of Rabbit Antibodies to Lipopolysaccharides Escherichia coli J5 and Other Gram-Negative Bacteria Get access George R. Siber, Siber Search for other works by this author on: Oxford Academic PubMed Google Scholar Stephen A. Kania, Kania H. Shaw Warren The Infectious Diseases, Volume 152, Issue 5, November 1985, Pages 954–964, https://doi.org/10.1093/infdis/152.5.954 Published: 01 1985 history Received: 23 January Revision received: 07 May
Abstract To determine the zoonotic risk from meticillin‐resistant staphylococcal species or transfer of resistance genes between dogs with pyoderma and their owners, 25 dog–owner pairs were studied. Cultures obtained dog’s lesions owner’s nasal cavity on initial visit. Staphylococcus isolates identified antimicrobial susceptibility tests performed by Kirby‐Bauer disk diffusion method. Presence mecA gene was determined PCR. Staphylococcal Cassette Chromosome (SCC mec ) typing multiplex...
The ability of convalescent serum to passively protect calves against Haemophilus somnus-induced pneumonia was studied. Preimmune and were obtained from before or after recovery experimental chronic H. somnus pneumonia. Passive protection assessed in another group by intrabronchial inoculation that had been incubated with preimmune serum. Each calf inoculated each treatment alternating caudal lung lobes. Twenty-four hours almost no present lungs bacteria serum, whereas severe Quantitation...
Polymyxin nonapeptides, proteolytic derivatives of polymyxin antibiotics, are less toxic than their parent compounds but retain some antibacterial activities. To confirm and expand observations that nonapeptides have anti-endotoxin activity, we studied the ability colistin nonapeptide to bind bacterial lipopolysaccharide (LPS) inhibit effects LPS on Limulus amoebocyte lysate lymphocyte mitogenicity. Colistin was purified by high-pressure liquid chromatography demonstrated equilibrium...
To quantify the neutralization of bacterial lipopolysaccharide (LPS) by human plasma, dilutions Escherichia coli O113 LPS were incubated with followed addition Limulus amebocyte lysate (LAL). The reaction between and LAL was monitored spectrophotometrically, concentration resulting in 50% response (LR50) determined. Analysis 145 outdated plasma samples yielded a range LR50 6 1,500 ng/ml. Pools high low prepared. pool neutralized 166-fold more E. 0113 LPS, 190-fold 0111B4 42-fold Klebsiella...
Squamous cell carcinoma (SCC) is the most common malignant cutaneous and oral neoplasm of cats. Papillomavirus (PV) DNA has been identified in a proportion feline Bowenoid situ carcinomas (BISCs), SCCs single SCC, but its exact role pathogenesis remains unknown. In humans, it suggested that ultraviolet (UV) light human PV (HPV) may act as cofactors SCC carcinogenesis. Little known about influence UV on prevalence lesions, including actinic keratosis (AK). Additionally, noncutaneous largely...
Free-ranging caribou and moose populations in some regions of Alaska undergo periodic declines numbers. Caribou are managed by the state as valuable resources for not only sustenance subsistence, but also cultural heritage. Incidence prevalence diseases that may impact herd health recruitment from year to relevant management decisions aimed protect long-term viability these herds. Neospora caninum Toxoplasma gondii two apicomplexan parasites can cause neurologic disease abortions their...
Squamous cell carcinoma (SCC) is the most common tumor arising in periocular and penile areas of horses. Both ultraviolet radiation papillomaviruses have been implicated pathogenesis SCC various species, including horse. This retrospective study used polymerase chain reaction (PCR) to detect papillomavirus DNA archival biopsy samples from equine 3 different geographic (northeast, southeast, central United States). Forty-two SCCs were tested; none contained DNA. Twenty-two tested, was...
Foods that contain antibiotic residues have potential adverse health effects on consumers and provide selective pressure for the threat of antimicrobial resistance (AMR). This study's objective was to measure tetracycline, sulfonamide, erythromycin in beef, eggs, honey sold as "antibiotic-free" at farmers' markets East Tennessee (East TN) United States (U.S.). Between July September 2020, 36 food products (9 18 egg, 9 products) were purchased from TN tested using competitive enzyme-linked...