A5089307549- Antimicrobial Resistance in Staphylococcus
- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Bacterial biofilms and quorum sensing
- Genomics and Phylogenetic Studies
- Dermatology and Skin Diseases
- Bacterial Identification and Susceptibility Testing
- Viral Infections and Outbreaks Research
- Biochemical and Structural Characterization
- Viral Infections and Vectors
- Gut microbiota and health
- Bacillus and Francisella bacterial research
- SARS-CoV-2 and COVID-19 Research
- Microbial Metabolism and Applications
- vaccines and immunoinformatics approaches
- Probiotics and Fermented Foods
- Exercise and Physiological Responses
- Streptococcal Infections and Treatments
- Allergic Rhinitis and Sensitization
- Identification and Quantification in Food
- Biological Research and Disease Studies
- Advancements in Transdermal Drug Delivery
- Healthcare and Environmental Waste Management
- SARS-CoV-2 detection and testing
- Antibiotic Resistance in Bacteria
Suez Canal University
2016-2025
Sinai University
2021-2025
American Pharmacists Association
2019
Nahda University
2019
University of Tennessee at Knoxville
2009-2017
Knoxville College
2012
ABSTRACT Staphylococcus pseudintermedius is an opportunistic pathogen in dogs. Four housekeeping genes with allelic polymorphisms were identified and used to develop expanded multilocus sequence typing (MLST) scheme. The new seven-locus technique shows S. have greater genetic diversity than previous methods discriminates more isolates based upon host origin.
Marine invertebrates-associated microorganisms were considered to be important sources of marine bioactive products. This study aims isolate invertebrates associated bacteria with antimicrobial activity from the Red Sea and test their biosynthetic potential through detection PKS NRPS gene clusters involved production secondary metabolites. In this respect, fifty bacterial strains isolated eight different screened for against standard pathogenic (
Abstract SARS-CoV-2 caused a global panic among populations. Rapid diagnostic procedures for the virus are crucial disease control. Thus, designed signature probe from highly conserved region of was chemically immobilized onto nanostructured-AuNPs/WO 3 -screen printed electrodes. Different concentrations matched oligonucleotides were spiked to test specificity hybridization affinity whereas electrochemical impedance spectroscopy used tracking performance. After full assay optimization,...
Extraction of bacterial DNA is a critical step in obtaining high-quality DNA. The overall quality, yield, and integrity the genomic isolated affects success any downstream applications. Many factors can affect quality yield obtained. By understanding some key steps underlying chemical physical properties, methodology extraction be modified to deal with specific sample types for particular research purposes. This narrative review aimed present comprehensive overview protocols by summarizing...
An effective vaccine against Staphylococcus aureus infection is a major planetary heath priority, particularly with increasing antibiotic resistance worldwide. Previous efforts for highly S. were largely unsuccessful, in part, because the designs have tended to target mainly B cell immunity and development of opsonic antibodies. In contrast, recent observations suggest that mediated may be critical protection aureus. addition, surface proteins are among key immunodominant antigens they first...
Staphylococcus pseudintermedius is the primary cause of canine pyoderma and has been associated with diseases in other animals, including human beings. A high prevalence methicillin multidrug resistance reported this bacterium some geographic regions United States. Multilocus sequence type (MLST) 68 was implicated, initially, as major clonal genotype based on a limited number samples. The objectives study were to determine population genetics S. isolated from cross-section States using...
Atopic dermatitis (AD) is a complex, multifactorial, chronic pruritic inflammatory skin disease. We report the first microbiome study and new insights on relationship between microbiota variation AD susceptibility in population sample from Egypt. characterized 75 patients with 20 healthy controls using Illumina MiSeq sequencing of 16S rRNA gene. Overall, bacterial diversity was less than those subjects. Genus level analysis revealed significant abundance variations by age, disease severity,...
Microbial communities residing in the nose play important roles human health and disease. We report marked differences nasal microbiota between a rural community an industrial setting located near major urban city. Nasal samples were collected from 19 healthy male subjects: 9 persons living village, 10 ceramic factory workers Egyptian The sample had higher distinct diversity compared with exposed to pollution daily. Taxonomic analysis of sequences revealed five phyla; among these phyla...
Measles virus considers an important cause of child morbidity and mortality in some areas as Africa. Ribavirin's activity a nucleoside analog can disclose the surprisingly broad spectrum action against several RNA viruses under laboratory cell culture conditions. The Current study aimed to investigate antiviral ribavirin Nano gold particles (AuNPs) measles on vero line. Ribavirin- AuNPs was prepared, characterization cytotoxicity ribavirin, -AuNPs were tested cells using MTT assay. Antiviral...
Pseudomonas aeruginosa (P. aeruginosa) is considered as the foremost cause of hospital-acquired infections due to its innate and plasmid-mediated resistance multiple antibiotics making it a multi-drug resistant (MDR) pathogen. This study aimed determine biofilm formation ability presence different virulence factors genes (pslA, pelA, exoS, toxA algD) among biofilm-forming strains P. clinical isolates from burn units in Ismailia Hospitals, Egypt. In our cross-sectional study, one hundred...
Objective: The main purpose of this work was to prepare tolnaftate (TOL) loaded nanostructured lipid carriers (NLCs), Evaluate its characteristics and in vitro release study.
 Methods: Tolnaftate Nanostructured were prepared by the high shear homogenization method using different liquid lipids types (DERMAROL DCO® DERMAROL CCT®) concentrations, concentration ratios tween80® span20® speeds. All formulated nanoparticles subjected particle size (PS), zeta potential (ZP), polydispersity...
Detection of biosurfactant activity have been proposed Previously by several studies either quantitatively or qualitatively. Quantitative methods give precise measurement the surface tension using accurate devices while qualitative measurements are considered screening for detection with lower precision. Due to high cost direct and in some cases unavailability devices, remain most common used tests surface-active properties. The need an accurate, cheap method was reason this trial developing...
The Red Sea is a promising habitat for the discovery of new bioactive marine natural products. Sponges associated microorganisms represent wealthy source compounds with unique chemical structures and diverse biological activities. Metagenomics an important omics-based culture-independent technique that used as effective tool to get genomic functional information on sponge symbionts. In this study, we metagenomic analysis two Egyptian sponges Hyrtios erectus Phorbas topsenti microbiomes study...
Atopic dermatitis (AD) is a chronic complex inflammatory skin disorder. AD characterized by multiple contributing factors, such as impairment in barrier integrity, participation of either innate or adaptive immunity arms, addition to the pivotal roles cutaneous microbiome. The analysis microbiota was performed using shotgun metagenomic sequencing on an Illumina HiSeq platform parallel with 16s rRNA genes MiSeq sequencing. Two samples for each technique were collected from one child and adult...