Urinary catheters are commonly associated with urinary tract infections. This study aims to inhibit bacterial colonisation and biofilm of catheters. Silicon catheter pieces were varnished green silver nanoparticles (AgNPs) using Pistacia lentiscus mastic prevent colonisation. Pomegranate rind extract was used synthesize AgNPs. AgNPs characterized by UV-Vis spectroscopy, X-ray crystallography, transmission electron microscopy (TEM). Results obtained revealed that the size most ranged between...

Inappropriate use of antibiotics in clinical settings is thought to have led the global emergence and spread multidrug-resistant pathogens. The goal this study was investigate prevalence genes encoding aminoglycoside resistance plasmid-mediated quinolone among isolates Klebsiella pneumoniae . All K. were phenotypically identified using API 20E then confirmed genotypically through amplification specific phoE gene. genotyped by enterobacterial repetitive intergenic consensus polymerase chain...

The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because the pan drug-resistant strains. objective this study was investigate existence 12 carbapenemase genes among CRKP clinical isolates.Ninety-six Klebsiella spp. isolates were collected. identified phenotypically genotypically. These screened for susceptibility 24 different antibiotics. modified Hodge test (MHT) Carba Nordmann/Poirel (NP) used screen strains production. Phenotypic...

The use of plants and their derived substances increases day by for the discovery new therapeutic agents owing to versatile applications. Conocarpus erectus L. (family Combretaceae) is one two species in genus widely spread Taif other parts Kingdom Saudi Arabia. It used some countries as folk remedy anemia, catarrh, conjunctivitis, diabetes, diarrhea fever. To our knowledge from literatures there little work about phytochemical contents biological activities C. which encouraged authors carry...

Abstract Background Methicillin-resistant Staphylococcus aureus is linked to both nosocomial and community infections. One of the key virulence factors S. Panton-Valentine leukocidin ( PVL ). The genes are mostly associated with community-acquired MRSA (CA-MRSA). This study evaluates prevalence as a marker for CA-MRSA at tertiary hospitals in Mansoura, Dakahlia, Egypt. was isolated from clinical specimens obtained different departments hospitals, outpatient clinics, hospital healthcare...

The extensive use of β-lactam antibiotics has led to emergence and spread extended-spectrum β-lactamases (ESBLs). This study was conducted investigate the prevalence 7 different ESBL genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES, blaOXA-10) O25b-ST131 high-risk clone among 61 clinical isolates Escherichia coli. Also, one broad-spectrum β-lactamase (blaOXA-1) investigated. also constructed evaluate iodometric overlay method in detection production. Phenotypic identification E. coli...

Background: Quinolones are among the most effective antibiotics against Pseudomonas spp. Several chromosomal and/or plasmid-mediated quinolone-resistance mechanisms have been found in . Plasmid-mediated (PMQR) is mediated by (QNR) proteins, modifying enzymes or efflux pumps. Only a few previous studies examined prevalence of Kingdom Saudi Arabia (KSA) and showed it increasing. Mechanisms KSA; herein; not extensively studied. Methods: Ninety-two isolates were collected their resistance to...

Recently, the residues or by-products of agriculture industry take attention for their valuable source natural antioxidants. Taif rose (Rosa damascena trigintipetala Dieck) is considered one most important economic products Taif, Saudi Arabia. In this study, antioxidant and total phenolic contents defatted flowers fresh were investigated. The activity was chemically determined using three methods; 1,1-diphenyl picrylhydrazyl (DPPH.) free radical scavenging activity, phosphomolybdenum method...

Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen, which developed multi-drug-resistance to different classes of antibiotics including carbapenems. This study examined ten carbapenemase genes among 32 carbapenem-resistant A. clinical isolates recovered from Taif, Saudi Arabia.Isolates were phenotypically identified the genus level by Vitek®2 and API 20NE®. The species was confirmed amplification blaOXA-51. susceptibility for 21 performed Vitek 2 modified Kirby-Bauer...

Abstract Objective This study was conducted to evaluate the antimicrobial efficacy and mechanical properties of two experimental self-disinfecting alginate preparations utilising different agents; povidone-iodine silver nanoparticles. Methods Alginate moulds were assigned into three groups. Additives-free control group, povidone-iodine-containing nanoparticle-containing group. Discs from each group obtained assessed for their activities by disc diffusion method against Staphylococcus aureus...

SUMMARY: Bacillus subtilis mutants with altered penicillin-binding proteins (PBPs), or expression of PBPs, were isolated by screening for changes in susceptibility to β-lactam antibiotics. Mutations affecting only PBPs 2a, 2b and 3 isolated. Cell shape peptidoglycan metabolism examined representative mutants. Cells a PBP 2a mutant (UB8521) usually twisted whereas (UB8524) (UB8525) produced helices, particularly after growth at 41 °C. The had higher synthetic activity than its parent strain...

Frankia strains isolated from Saudi Arabia, reported for the first time, were identified based on morphological and molecular tools compared to those Egypt. All displayed typical characterization of represented by branched hyphae, production vesicles sporangia. The phylogenetic analysis relationships among investigated comparing 16S rRNA gene sequences. revealed three genetic groups which formed two clusters. cluster was composed eight subdivided into (one group containing five strains; CgIT...

Rose is one of the most economically important ornamental crops in world. In this study, we analyzed nine macro- and microelements soils petals R. damascena Mill. cultivated Shafa Hada mountains. The amounts investigated varied from soil or petal sample to another they were generally higher samples compared Hada. On other hand, levels elements not dependent on their samples. While water extracts farms slightly alkaline (pH 7.69), moderately 8.04) farms. oil produced by rose relatively larger...

The composition of membranes from whole cells and minicells Bacillus subtilis strain BD474 was examined. Two-dimensional electrophoresis showed that the content several individual polypeptides differed in two membrane types. Whole cell appeared to contain more penicillin binding protein (PBP) 2a, glycerol-3-phosphate dehydrogenase, succinate dehydrogenase NADH than minicell membranes, but less PBP2b, lactate malate dehydrogenase. Glycerol-3-phosphate were unstable, other enzymes PBPs stable...

<title>Abstract</title> OBJECTIVE: This study was carried out to evaluate the antimicrobial efficacy and physical properties of two experimental self-disinfecting alginate preparations. METHODS: The molds were divided into three groups: control one without any additives, others contained either povidone-iodine (PV-I) or silver nanoparticles (AgNPs). Samples from each group assessed for their activity by disc diffusion method against <italic>Staphylococcus aureus</italic> (Gram-positive),...

18Feb 2017 THE EFFECT OF ALKALOIDS, SAPONINSAND THYMOQUINONE NIGELLA SATIVA SEEDS ON BIOFILM PRODUCTION, MOTILITY, OUTER MEMBRANE PROTEINS AND LIPOPOLYSACCHARIDE SOME BACTERIA. Mohamed Shohayeb and Eman Halawani. Taif University, Faculty of pharmacy, Department microbiology, Taif, Saudi Arabia. Science, Biology ,