Fungal keratitis (FK) is a serious pathogenic condition usually associated with significant ocular morbidity. Natamycin (NAT) the first-line and only medication approved by Food Drug Administration for treatment of FK. However, NAT suffers from poor corneal penetration, which limits its efficacy treating deep keratitis.The objective this work was to prepare solid lipid nanoparticles (NAT-SLNs) achieve sustained drug release increased penetration.NAT-SLNs were prepared using...

Inappropriate use of antibiotics in clinical settings is thought to have led the global emergence and spread multidrug-resistant pathogens. The goal this study was investigate prevalence genes encoding aminoglycoside resistance plasmid-mediated quinolone among isolates Klebsiella pneumoniae . All K. were phenotypically identified using API 20E then confirmed genotypically through amplification specific phoE gene. genotyped by enterobacterial repetitive intergenic consensus polymerase chain...

The emergence of carbapenem-resistant K. pneumoniae (CRKP) continues to escalate and is alarming because the pan drug-resistant strains. objective this study was investigate existence 12 carbapenemase genes among CRKP clinical isolates.Ninety-six Klebsiella spp. isolates were collected. identified phenotypically genotypically. These screened for susceptibility 24 different antibiotics. modified Hodge test (MHT) Carba Nordmann/Poirel (NP) used screen strains production. Phenotypic...

The extensive use of β-lactam antibiotics has led to emergence and spread extended-spectrum β-lactamases (ESBLs). This study was conducted investigate the prevalence 7 different ESBL genes (blaTEM, blaSHV, blaCTX-M, blaVEB, blaPER, blaGES, blaOXA-10) O25b-ST131 high-risk clone among 61 clinical isolates Escherichia coli. Also, one broad-spectrum β-lactamase (blaOXA-1) investigated. also constructed evaluate iodometric overlay method in detection production. Phenotypic identification E. coli...

Background: Quinolones are among the most effective antibiotics against Pseudomonas spp. Several chromosomal and/or plasmid-mediated quinolone-resistance mechanisms have been found in . Plasmid-mediated (PMQR) is mediated by (QNR) proteins, modifying enzymes or efflux pumps. Only a few previous studies examined prevalence of Kingdom Saudi Arabia (KSA) and showed it increasing. Mechanisms KSA; herein; not extensively studied. Methods: Ninety-two isolates were collected their resistance to...

Acinetobacter baumannii (A. baumannii) is a common nosocomial pathogen, which developed multi-drug-resistance to different classes of antibiotics including carbapenems. This study examined ten carbapenemase genes among 32 carbapenem-resistant A. clinical isolates recovered from Taif, Saudi Arabia.Isolates were phenotypically identified the genus level by Vitek®2 and API 20NE®. The species was confirmed amplification blaOXA-51. susceptibility for 21 performed Vitek 2 modified Kirby-Bauer...

The recent pandemic caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has opened the door to potential threats of system. discovery drugs from natural sources is one most important strategies for treating upper tract. In this study, we investigated selected formulated EOs activities against Gram-negative (E. coli, K. pneumonia, and P. aeruginosa) Gram-positive (S. aureus, E. fecalis) bacteria SARS-CoV-2 virus, with mode action as anti-SARS-CoV-2. Cinnamomum zeylanicum...

Schistosomiasis comes after malaria as the second most important fatal parasitic disease. In Saudi Arabia Schistosoma mansoni is endemic in Jazan, Bishah, Aseer, Madina, Al-Bahah and Taif. The methods used for diagnosis of schistosomiasis are complex, time-consuming, require extra instruments and/or need skillful persons; therefore, there a development sensitive easy method. this study, first time, novel screen-printed immunosensor detection S. antibodies (ABs) was developed. Soluble worm...

Frankia strains isolated from Saudi Arabia, reported for the first time, were identified based on morphological and molecular tools compared to those Egypt. All displayed typical characterization of represented by branched hyphae, production vesicles sporangia. The phylogenetic analysis relationships among investigated comparing 16S rRNA gene sequences. revealed three genetic groups which formed two clusters. cluster was composed eight subdivided into (one group containing five strains; CgIT...

Background: Acinetobacter baumannii ( A. ) is one of the most important nosocomial pathogens responsible for a wide range infections. Aim: This study aimed to investigate existence plasmidic genes encoding aminoglycoside modifying enzymes (AMEs), 16S rRNA methyltransferases (RMT), and altered dihydropetroate synthase (DHPS) encoded by sul1 gene among clinical isolates collected from Taif, Kingdom Saudi Arabia (KSA). The mutations in aac(6ʹ)-Ib were also investigated. Methods: Forty...

A selected set of new N 1- (6-fluoro-4-oxoquinolin-8-yl)amidrazone-3-carboxylates (8a_e) incorporating Npiperazines and related congeners has been synthesized by reacting the hydrazonoyl chloride, derived from 8-amino-6- fluoro-4-oxoquinloine-3-carboxylate, with appropriate sec-cyclic amine. Their suggested structures are supported elemental analysis, 1H NMR, 13C NMR ESI-HRMS spectral data. These novel compounds were screened for their antitumor antibacterial activity. While no significant...

Up to day, the role of ultraviolet rays in producing cancers like melanoma has been considered extensively. However, quantum effects on evolutions discussed minorly. In this research, we propose a model for replication DNAs within skin cells. We show that prevent imaging information genes parent cells child and make some genetic errors produced during replications. compare with experimental data obtain growth rate tumour skin.

Reports examine quinolone resistance mechanisms among

Discovery of novel pyridazinone derivatives with dual antimicrobial and anticancer activities.

Background and Objectives: Alzheimer’s disease (AD) is the most common neurodegenerative disorder in world. Due to failure of traditional drugs produce a complete cure for AD, search new safe effective lines therapy has attracted attention ongoing research. Canagliflozin an anti-diabetic agent with proven efficacy treatment neurological disorders which mitochondrial dysfunction, oxidative stress, apoptosis, autophagy play pathophysiological role. Elucidation potential effects different doses...

2-pyridyl [3H]-quinazolin-4-one derivatives fused or substituted with different oxygen nitrogen heterocycle moieties as potential anti-tumor and anti-microbial agents were prepared, characterized biologically screened. The synthesis process started from 5-bromo-2-[pyridin-4-ylcarbonyl]amino]benzoic acid which was converted to the crucial building block 6-Bromo-2-(pyridin-4-yl)quinazolin-4(3H)-one via two alternative routes. Compound 3 underwent halogenation reaction POCl3 PCl5 afford...

Schistosomiasis ranks second behind malaria in terms of overall morbidity and mortality. We evaluated the lethal effect Punica granatum ellagitannins, extracted from fruit rind, placenta barks root stem, on adult worms Schistosoma mansoni (S. mansoni). All four ellagitannins were to S. worms. However, while rind most potent, placental least. Rind capable killing 40% at a concentration 25µg/mL after 5 days. The 100% was achievable by 50µg/mL LD50S 96h 120h 41.25 µg/mL 28.73 respectively....

DNA could emit some waves and absorb or repel drugs. Thus, have the main role in drug's effects on cells. Recently, scientists shown that probability for survival patients who taken nivolumab plus ipilimumab is more than obtained alone. We show reason these events drug molecules which charges be absorbed by within propose a physical model calculating attractive forces between drugs In this model, formed from charged particles its motions along blood vessels, electrical currents are emerged....

Seventy clinical isolates of Escherichia coli were obtained from two tertiary care hospitals in Taif Governorate, Saudi Arabia and investigated for their susceptibility to 15 antibiotics different classes using antibiotic disc diffusion method.Amikacin imipenem the most active while, ampicillin was least active.Multiple drug resistance detected 52.9% which resistant 3-6 three or more classes.All tested ability produce β-lactamases general extended spectrum β-lactamase particular by...