A5065501799- Neuroscience and Neuropharmacology Research
- Memory and Neural Mechanisms
- Neural dynamics and brain function
- Neurogenesis and neuroplasticity mechanisms
- CRISPR and Genetic Engineering
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- Sleep and Wakefulness Research
- Nicotinic Acetylcholine Receptors Study
- Cellular Automata and Applications
- Receptor Mechanisms and Signaling
- RNA Interference and Gene Delivery
- Ubiquitin and proteasome pathways
- Neuroscience and Neural Engineering
- Planarian Biology and Electrostimulation
- Alzheimer's disease research and treatments
- Amyotrophic Lateral Sclerosis Research
- Neonatal Respiratory Health Research
- Neonatal and fetal brain pathology
- Advanced Memory and Neural Computing
- Glioma Diagnosis and Treatment
- Neuroscience, Education and Cognitive Function
- Genetics and Neurodevelopmental Disorders
- Pluripotent Stem Cells Research
- Retinal Development and Disorders
Norwegian University of Science and Technology
2018-2024
Kavli Institute for Systems Neuroscience
2019-2022
Rajiv Gandhi Centre for Biotechnology
2018-2020
University of Kerala
2018-2020
University of Bergen
2009-2017
Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CLCX), this has yet be fully accomplished. The CLCX is generally considered comprise two major subdivisions, (CL) and dorsal endopiriform nucleus (DEn), regional boundaries these areas debated. To address this, we conducted multifaceted analysis of fiber- cytoarchitecture, genetic marker expression, connectivity using mice both sexes, create comprehensive...
Activity-regulatedcytoskeleton-associated protein (Arc) is implicated as a master regulator of long-term forms synaptic plasticity and memory formation, but the mechanisms controlling Arc function are little known. Post-translation modification by small ubiquitin-like modifier (SUMO) proteins has emerged major mechanism for regulating protein-protein interactions function. We first show in cell lines that ectopically expressed undergoes mono-SUMOylation. The covalent addition single SUMO1...
Although a variety of remarkable molecular tools for studying neural circuits have recently been developed, the ability to deploy them in particular neuronal subtypes is limited by fact that native promoters are almost never specific enough. We showed one can generate transgenic mice with anatomical specificity surpassing combining enhancers uniquely active brain regions heterologous minimal promoter, an approach we call EDGE (Enhancer-Driven Gene Expression). Here extend this strategy...
Fan cells in layer II of the lateral entorhinal cortex (LEC) form a main component projection to dentate gyrus, CA3 and CA2 hippocampal formation. This has counterpart originating from stellate medial (MEC). Available evidence suggests that two pathways carry different information, exemplified by difference spatial tuning LEC MEC. The grid cell, prominent position-modulated cell type present MEC, been postulated derive its characteristic hexagonal firing pattern dominant disynaptic...
Projection neurons in the anteriolateral part of entorhinal cortex layer II are predominant cortical site for hyper-phosphorylation tau and formation neurofibrillary tangles prodromal Alzheimer's disease. A majority projection unique among excitatory by expressing protein reelin. In disease, these reelin-expressing prone to accumulate intracellular amyloid-β, which is mimicked a rat model that replicates spatio-temporal cascade Two important findings relation this reelin-signalling...
The entorhinal cortex, in particular neurons layer V, allegedly mediate transfer of information from the hippocampus to neocortex, underlying long-term memory. Recently, this circuit has been shown comprise a hippocampal output recipient Vb and cortical projecting Va. With use vitro electrophysiology transgenic mice specific for Vb, we assessed presence thus necessary connection Vb-to-Va functionally distinct medial (MEC) lateral (LEC) subdivisions; MEC, particularly its dorsal part,...
Abstract GABAergic neurons represent 10–15% of the neuronal population cortex but exert a powerful control over information flow in cortical circuits. The largest class neocortex is represented by parvalbumin‐expressing fast‐spiking neurons, which provide somatic inhibition to their postsynaptic targets. Recently, density parvalbumin interneurons has been shown be lower associative areas mouse as compared with sensory and motor areas. Modelling work based on these quantifications linked...
Abstract The NMDA receptors are a type of glutamate receptors, which is involved in neuronal function, plasticity and development the mammalian brain. However, how contribute to adult neurogenesis dentate gyrus unclear. In this study, we investigate question by examining region-specific knock-out mouse line that lacks NR1 gene, encodes essential subunit granule cells (DG-NR1KO mice). We found survival newly-generated cells, cell proliferation size layer significantly reduced dorsal DG-NR1KO...
The wide diversity of cortical inhibitory neuron types populating the cortex allows assembly diverse microcircuits and endows these circuits with different computational properties. Thus, characterizing neuronal is fundamental to describe building blocks probe their function. To this purpose, mouse has emerged as a powerful tool genetically label manipulate specific cell-types in mammalian brain. Among cell-types, parvalbumin-expressing interneuron type (PV-INs) perhaps most characterized....
Accurate anatomical characterizations are necessary to investigate neural circuitry on a fine scale, but for the rodent claustrum complex (CC) this has yet be fully accomplished. The CC is generally considered comprise two major subdivisions, (CL) and dorsal endopiriform nucleus (DEn), regional boundaries these areas highly debated. To address this, we conducted multifaceted analysis of fiber- cyto-architecture, genetic marker expression, connectivity using mice both sexes, create...
Activity-regulated cytoskeleton-associated protein, Arc, is a major regulator of long-term synaptic plasticity and memory formation. Here we reveal novel interaction partner resident endoplasmic reticulum transmembrane calnexin. We show an between recombinantly-expressed GST-tagged Arc endogenous calnexin in HEK293, SH-SY5Y neuroblastoma PC12 cells. The was dependent on the central linker region protein that also required for endocytosis AMPA-type glutamate receptors. High-resolution...
Current preclinical models of neurodegenerative disease, such as amyotrophic lateral sclerosis (ALS), can significantly benefit from in vitro neuroengineering approaches that enable the selective study and manipulation neurons, networks, functional units interest. Custom-designed compartmentalized microfluidic culture systems co-culture different relevant cell types interconnected but fluidically isolated microenvironments. Such thus be applied for ALS disease modeling, they recapitulation...
Summary Understanding brain function requires understanding neural circuits at the level of specificity which they operate. While recent years have seen development a variety remarkable molecular tools for study circuits, their utility is currently limited by inability to deploy them in specific elements native i.e. particular neuronal subtypes. One can obtain degree with neuron-specific promoters, but promoters are almost never sufficiently restricting this approach transgenic animals. We...
Accurate investigations of neural circuitry require specific genetic access to individual circuit elements, i.e., the myriad neuronal cell-types in brain. However, native promoters cannot achieve this because while most genes are expressed brain, few a single cell-type. We recently used enhancers, subcomponents transcriptional apparatus which tell when and where express, combined with heterologous minimal increase specificity transgene expression, an approach we call Enhancer-Driven Gene...
While a variety of remarkable molecular tools for studying neural circuits have recently been developed, the ability to deploy them in particular neuronal subtypes is limited by fact that native promoters are almost never specific enough. We showed one can generate transgenic mice with anatomical specificity surpassing combining enhancers uniquely active brain regions heterologous minimal promoter, an approach we call EDGE (Enhancer-Driven Gene Expression). Here extend this strategy...
Summary The entorhinal cortex, in particular neurons layer V, allegedly mediate transfer of information from the hippocampus to neocortex, underlying long-term memory. Recently, this circuit has been shown comprise a hippocampal output recipient Vb and cortical projecting Va. With use vitro electrophysiology transgenic mice specific for Vb, we assessed presence thus necessary connection Vb-to-Va functionally distinct medial (MEC) lateral (LEC) subdivisions; MEC, particularly its dorsal part,...
Abstract GABAergic neurons represent 10-15% of the neuronal population cortex but exert a powerful control over information flow in cortical circuits. show an extraordinary diversity their morphology, physiology, molecular markers and connectivity. This allows to participate wide variety microcircuit motifs. The has been shown be conserved across regions. can broadly divided three major classes parvalbumin, somatostatin 5HT3aR groups. largest class is represented by parvalbumin-expressing...
Neuronal firing patterns are the result of inputs converging onto single cells. Identifying these inputs, anatomically and functionally, is essential to understand how neurons integrate information. Single-cell electroporation helper genes subsequent local injection recombinant rabies viruses enable precise mapping individual cells in superficial layers intact cortex. However, access deeper structures requires more invasive procedures, including removal overlying tissue. We developed a...