A5068556181- Neuroscience and Neuropharmacology Research
- Photoreceptor and optogenetics research
- Neuroinflammation and Neurodegeneration Mechanisms
- Receptor Mechanisms and Signaling
- Neural dynamics and brain function
- Lipid Membrane Structure and Behavior
- Cellular transport and secretion
- Neurogenesis and neuroplasticity mechanisms
- Mitochondrial Function and Pathology
- Birth, Development, and Health
- Memory and Neural Mechanisms
- Diet and metabolism studies
- Photochromic and Fluorescence Chemistry
- Analytical Chemistry and Sensors
- Sleep and Wakefulness Research
- Neuroscience and Neural Engineering
- Electrochemical sensors and biosensors
- Advanced biosensing and bioanalysis techniques
- Cell Image Analysis Techniques
- Advanced Fluorescence Microscopy Techniques
- Retinal Development and Disorders
- Circadian rhythm and melatonin
- Advanced Chemical Sensor Technologies
- Metabolomics and Mass Spectrometry Studies
- Tryptophan and brain disorders
University of California, San Francisco
2016-2025
Arcadia Science
2023
Arcadia
2023
Chan Zuckerberg Initiative (United States)
2022
Kavli Institute for Theoretical Sciences
2018-2020
Northeastern University
2020
Columbia University
2014-2016
Howard Hughes Medical Institute
2007-2011
Icahn School of Medicine at Mount Sinai
2003-2004
The role of astrocytes in neuronal function has received increasing recognition, but disagreement remains about their at the circuit level. Here we use vivo two-photon calcium imaging neocortical while monitoring activity state local electrophysiologically and optically. We find that astrocytic precedes spontaneous shifts to slow-oscillation-dominated state, a rhythm characterized by synchronized firing important for sleep memory. Further, show optogenetic activation switches this...
Non-rapid eye movement (NREM) sleep, characterized by slow-wave electrophysiological activity, underlies several critical functions, including learning and memory. However, NREM sleep is heterogeneous, varying in duration, depth, spatially across the cortex. While these features are thought to be largely independently regulated, there also evidence that they mechanistically coupled. To investigate how cortical controlled, we examined astrocytic network, comprising a cortex-wide syncytium...
Abstract Cortical state, defined by population-level neuronal activity patterns, determines sensory perception. While arousal-associated neuromodulators—including norepinephrine (NE)—reduce cortical synchrony, how the cortex resynchronizes remains unknown. Furthermore, general mechanisms regulating synchrony in wake state are poorly understood. Using vivo imaging and electrophysiology mouse visual cortex, we describe a critical role for astrocytes circuit resynchronization. We characterize...
Abstract Astrocytes, the most abundant non-neuronal cell type in mammalian brain, are crucial circuit components that respond to and modulate neuronal activity through calcium (Ca 2+ ) signalling 1–7 . Astrocyte Ca is highly heterogeneous occurs across multiple spatiotemporal scales—from fast, subcellular 3,4 slow, synchronized connected astrocyte networks 8–10 —to influence many processes 5,7,11 However, inputs drive network dynamics remain unclear. Here we used ex vivo two-photon imaging...
The synchronization of neuronal assemblies during cortical UP states has been implicated in computational and homeostatic processes, but the mechanisms by which this occurs remain unknown. To investigate potential roles astrocytes synchronizing circuits, we electrically activated while monitoring activity surrounding network with electrophysiological recordings calcium imaging. Stimulating a single astrocyte activates other local circuit can trigger state synchronizations neighboring...
Synthetic photochromic compounds can be designed to control a variety of proteins and their biochemical functions in living cells, but the high spatiotemporal precision tissue penetration two-photon stimulation have never been investigated these molecules. Here we demonstrate excitation azobenzene-based protein switches versatile strategies enhance photochemical responses. This enables new applications activation neurons astrocytes with cellular subcellular resolution.
The prefrontal cortex (PFC) is a hub for cognitive control, and dopamine profoundly influences its functions. In other brain regions, astrocytes sense diverse neurotransmitters neuromodulators and, in turn, orchestrate regulation of neuroactive substances. However, basic physiology PFC astrocytes, including which neuromodulatory signals they respond to how contribute function, unclear. Here, we characterize divergent signaling signatures mouse the primary sensory cortex, show differential...
SUMMARY Optical recording of intricate molecular dynamics is becoming an indispensable technique for biological studies, accelerated by the development new or improved biosensors and microscopy technology. This creates major computational challenges to extract quantify biologically meaningful spatiotemporal patterns embedded within complex rich data sources, many which cannot be captured with existing methods. Here, we introduce Activity Quantification Analysis (AQuA2), a fast, accurate,...
Serotonin, or 5-hydroxytryptamine (5HT), is an important neurotransmitter in the nervous system of both vertebrates and invertebrates. Deficits 5HT signaling are responsible for many disabling psychiatric conditions, its molecular machinery target pharmaceuticals. We present a new phototrigger, compound [Ru(bpy)2(PMe3)(5HT)]2+, where PMe3 trimethylphosphine. As with other ruthenium-bipyridyl based caged compounds, [Ru(bpy)2(PMe3)(5HT)]2+ presents activity visible region spectrum....
Optical recording of intricate molecular dynamics is becoming an indispensable technique for biological studies, accelerated by the development new or improved biosensors and microscopy technology. This creates major computational challenges to extract quantify biologically meaningful spatiotemporal patterns embedded within complex rich data sources, many which cannot be captured with existing methods. Here, we introduce activity quantification analysis (AQuA2), a fast, accurate, versatile...
Thalamocortical axons are precisely targeted to cortical layer IV, but the identity of specific molecules that govern establishment laminar specificity in thalamocortical projection has been elusive. In this study, we test role N-cadherin, a homophilic cell adhesion molecule, targeting using cocultured thalamic and slice explants exposed N-cadherin function-blocking antibodies or inhibitory peptides. untreated cocultures, labeled normally grow stop forming terminal-like arbors....
We present a structured matrix factorization approach to analyzing calcium imaging recordings of large neuronal ensembles. Our goal is simultaneously identify the locations neurons, demix spatially overlapping components, and denoise deconvolve spiking activity each neuron from slow dynamics indicator. The relies on observation that spatiotemporal fluorescence can be expressed as product two matrices: spatial encodes location in optical field temporal characterizes concentration over time....
Optical nanoparticle (NP)-based sensors have been widely implemented as tools for detection of targeted ions and biomolecules. The NP sensing platform offer a modular design that can incorporate different components greater target specificity the ability to tune dynamic range, well encapsulation multiple dyes generate ratiometric signal with varying spectra. Despite these advantages, demonstrating quantitative ion imaging intracellular measurement still possess major challenge. Here, we...