A5050067141- Monoclonal and Polyclonal Antibodies Research
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
- Lymphoma Diagnosis and Treatment
- Mycobacterium research and diagnosis
- Cell Adhesion Molecules Research
- Leprosy Research and Treatment
- Microtubule and mitosis dynamics
- Neuroscience and Neuropharmacology Research
- Cancer, Hypoxia, and Metabolism
- Erythrocyte Function and Pathophysiology
- Immune Cell Function and Interaction
- HER2/EGFR in Cancer Research
- Glycosylation and Glycoproteins Research
- Cutaneous lymphoproliferative disorders research
- Immunotherapy and Immune Responses
- Blood groups and transfusion
- Digestive system and related health
- Receptor Mechanisms and Signaling
- Infant Nutrition and Health
- Immune Response and Inflammation
- Electrochemical sensors and biosensors
- Neuroinflammation and Neurodegeneration Mechanisms
- Radiopharmaceutical Chemistry and Applications
- Immunotoxicology and immune responses
University of California, San Francisco
2022-2024
University of Oregon
2016-2022
University of Oxford
1973-2022
MRC Weatherall Institute of Molecular Medicine
2016-2022
University of California, Berkeley
2020
John Radcliffe Hospital
1985-2017
University of Southern California
1978-2006
Georgetown University
1999
U-M Rogel Cancer Center
1998
Southern California University for Professional Studies
1995-1997
The distribution of muramidase (lysozyme) in normal and pathological human tissues has been studied, using an immunohistological technique. enzyme was demonstrated a variety healthy tissues, including serous salivary acinar cells, lactating mammary tissue, Paneth renal tubular myeloid cells (including eosinophils), histiocytic cells. In the most striking positivity encountered reactive granulomatous conditions such as tuberculosis Crohn's disease. finding this study are related to previous...
A method is described for the demonstration of specific immunoglobulin in plasma cells and other lymphoid sections taken from routine surgical histology specimens which have been formalin fixed paraffin embedded. An indirect sandwich technique was employed using rabbit antihuman antisera (anti-K, L, G, A, M) a swine antirabbit serum Ig conjugated with horseradish peroxidase. The presence revealed by staining tissue-bound peroxidase-labelled antibody, having previously stained endogenous...
Thrombospondin-1 (TSP) is a 430-kd glycoprotein that an important component of the extracellular matrix and known to be potent inhibitor angiogenesis (i.e., formation new blood vessels) both in vitro vivo. Several reports suggest TSP possesses tumor suppressor function, possibly through its ability inhibit neovascularization. It has recently been shown expression enhanced by product p53 gene (also as TP53).We examined role recurrence overall survival patients with invasive bladder cancer. We...
Antigen retrieval (AR) incorporating high-temperature microwave (MW) heating of tissue sections before immunostaining is a revolutionary technique that can unmask the antigens in formalin-fixed sections, thus making them available for immunohistochemical staining. Although high temperature believed to be primary mechanism antigens, variety chemical solutions have been tested define an optimal AR solution. We hypothesis pH solution may influence quality by using seven different buffer at...
An immunoperoxidase staining technique was used for detecting three major iron-binding proteins (transferrin, ferritin, and lactoferrin) in routine histological paraffin sections of human tissue. Transferrin found mainly hepatocytes, a variety epithelial myoepithelial cells, renal tubular histiocytes. Ferritin most readily histiocytes liver with weaker reactions seen cells. Lactoferrin lactating breast tissue, bronchial glands, polymorphs, gastric duodenal The is potentially valuable...
The prefrontal cortex (PFC) is a hub for cognitive control, and dopamine profoundly influences its functions. In other brain regions, astrocytes sense diverse neurotransmitters neuromodulators and, in turn, orchestrate regulation of neuroactive substances. However, basic physiology PFC astrocytes, including which neuromodulatory signals they respond to how contribute function, unclear. Here, we characterize divergent signaling signatures mouse the primary sensory cortex, show differential...
The application of immunohistochemistry to routinely decalcified, celloidin-embedded human temporal bone sections has been hampered because antigen loss during processing the specimens. To our knowledge, there no published report date describing immunohistochemical staining such tissues suitable for examination by light microscopy. Here we a novel retrieval technique which can be successfully used stain variety antigens in formalin-fixed, trichloroacetic acid-decalcified, sections. new...
Leprosy is a chronic granulomatous disease with an immunologic spectrum in which lepromatous leprosy patients have defective cell-mediated immune responses, comparison to tuberculoid patients. Immunoregulatory aspects of this were investigated by using monoclonal antibodies interleukin 2 (IL 2), IL receptors (Tac), and T lymphocyte subpopulations immunoperoxidase techniques on frozen sections skin biopsy specimens from 10 A 2+ cells revealed markedly fewer (lep. 0.028% +/- 0.02 vs tub. 0.46%...
SUMMARY Optical recording of intricate molecular dynamics is becoming an indispensable technique for biological studies, accelerated by the development new or improved biosensors and microscopy technology. This creates major computational challenges to extract quantify biologically meaningful spatiotemporal patterns embedded within complex rich data sources, many which cannot be captured with existing methods. Here, we introduce Activity Quantification Analysis (AQuA2), a fast, accurate,...
Abstract Though used widely in cancer therapy, paclitaxel only elicits a response fraction of patients. A strong determinant tumor is the state microtubule dynamic instability. However, whether manipulation this physiological process can be controlled to enhance has not been tested. Here, we show previously unrecognized role microtubule-associated protein CRMP2 inducing bundling through its carboxy terminus. This activity significantly decreased when FER tyrosine kinase phosphorylates at...
The mechanism by which precursor and pre-B cells undergo differentiation is unclear; however, it known that growth factors play an important role in this maturation process. lymphokine, IL-4 has been shown to increase expression of class II Ag on B induce cell proliferation. In the murine system, induced into cells. order analyze development we have established vitro culture system for human bone marrow We found presence IL-4, normal can be differentiate absence proliferation with four days...
Molecular morphology seeks to integrate the traditional morphologic criteria of surgical pathology with immunohistochemical and in situ hybridization techniques that allow demonstration a variety molecules, proteins, RNA DNA tissue section. While immunohistochemistry has proven be successful for demonstrating lineage related biomarkers value diagnosis classification tumors, concerns have been raised periodically about validation reagents, overall reproducibility staining method,...
Background: To understand the basis of nervous system development, we must learn how multipotent progenitors generate diverse neuronal and glial lineages. We addressed this issue in zebrafish enteric (ENS), a complex network that regulates essential intestinal functions. Little is currently known about ENS progenitor subpopulations diversity. Results: identified temporally spatially dependent based on coexpression three genes for normal development: phox2bb, sox10 , ret . Our data suggest...
Application of an immunoperoxidase method to a study the central nervous system: preliminary findings in human formalin‐fixed material Central system matcrial from 40 patients has been studied using technique on paraffin‐embedded tissues, examine distribution immunoglobulins, transferrin, and lysozyme. In various pathological conditions there was evidence deposition immunoglobulins other serum proteins or myelin sheaths, neurons. dying without neurological disease similar faint staining...
Taxanes represent some of the most commonly used chemotherapeutic agents for ovarian cancer treatment. However, they are only effective in approximately 40% patients. Novel therapeutic strategies required to potentiate their effect and improve patient outcome. A hallmark many cancers is constitutive activation PI3K/AKT pathway, which drives cell survival metabolism. We discovered a striking decrease AKT activity coupled with significant reduction glucose 6-phosphate ATP levels during mitotic...