A5037552101- Spinal Cord Injury Research
- Microtubule and mitosis dynamics
- Plant Reproductive Biology
- Ovarian cancer diagnosis and treatment
- Cellular Mechanics and Interactions
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Nerve injury and regeneration
- Nerve Injury and Rehabilitation
- Neurogenesis and neuroplasticity mechanisms
- Stroke Rehabilitation and Recovery
- Cancer, Lipids, and Metabolism
- Tuberous Sclerosis Complex Research
- Kruppel-like factors research
- Cancer Genomics and Diagnostics
- CRISPR and Genetic Engineering
- Tissue Engineering and Regenerative Medicine
- Pluripotent Stem Cells Research
- Glycosylation and Glycoproteins Research
- Polysaccharides and Plant Cell Walls
- Cancer, Hypoxia, and Metabolism
- Spinal Dysraphism and Malformations
- Photosynthetic Processes and Mechanisms
- Force Microscopy Techniques and Applications
- Cervical and Thoracic Myelopathy
Fujian Medical University
2025
Shanghai Pulmonary Hospital
2025
Tongji University
2025
Florida International University
2022-2023
MRC Weatherall Institute of Molecular Medicine
2014-2022
University of Oxford
2014-2022
Fudan University
2021-2022
Zhongshan Hospital
2021-2022
Brown Foundation
2017-2022
Hasanuddin University
2021
Promotion of remyelination is an important therapeutic strategy to facilitate functional recovery after traumatic spinal cord injury (SCI). Transplantation neural stem cells (NSCs) or oligodendrocyte precursor (OPCs) has been used enhance SCI. However, the microenvironment in injured inhibitory for (OL) differentiation NSCs OPCs. Identifying signaling pathways that inhibit OL could lead new strategies and In present study, we show reactive astrocytes from rat their conditioned media adult...
Abstract A dynamic actin cytoskeleton is essential for pollen germination and tube growth. However, the molecular mechanisms underlying organization turnover of in remain poorly understood. Villin plays a key role formation higher-order structures from filaments regulation dynamics eukaryotic cells. It belongs to villin/gelsolin/fragmin superfamily binding proteins composed six gelsolin-homology domains at its core villin headpiece domain C terminus. Recently, several family members plants...
Abstract Cytoplasmic actin cables are the most prominent structures in plant cells, but molecular mechanism underlying their formation is unknown. The function of these cables, which proposed to modulate cytoplasmic streaming and intracellular movement many organelles plants, has not been studied by genetic means. Here, we show that Arabidopsis thaliana formin3 (AFH3) an nucleation factor responsible for longitudinal pollen tubes. AFH3 gene encodes a 785–amino acid polypeptide, contains...
Actin filaments are often arranged into higher-order structures, such as the longitudinal actin cables that generate reverse fountain cytoplasmic streaming pattern present in pollen tubes. While several binding proteins have been implicated generation of these cables, mechanisms regulate their dynamic turnover remain largely unknown. Here, we show Arabidopsis thaliana actin-depolymerizing factor7 (ADF7) is required for cables. In vitro biochemical analyses revealed ADF7 a typical ADF prefers...
Abstract The actin cytoskeleton is an important regulator of cell expansion and morphogenesis in plants. However, the molecular mechanisms linking to these processes remain largely unknown. Here, we report functional analysis rice (Oryza sativa) FH5 /BENT UPPERMOST INTERNODE1 (BUI1), which encodes a formin-type nucleation factor affects plant rice. bui1 mutant displayed pleiotropic phenotypes, including bent uppermost internode, dwarfism, wavy panicle rachis, enhanced gravitropic response....
Spinal cord injury (SCI) is a devastating neurological disease without effective treatment. To generate comprehensive view of the mechanisms involved in SCI pathology, we applied RNA-Sequencing (RNA-Seq) technology to characterize temporal changes global gene expression after contusive mice. We sequenced tissue samples from acute and subacute phases (2 days 7 injury) systematically characterized transcriptomes with goal identifying pathways genes critical pathology. The top enriched...
Cotton fiber development at the stages of elongation and secondary wall synthesis determines traits length strength. To date, mechanisms controlling progression these two phases remain elusive. In this work, function a fiber-preferential actin-binding protein (GhPFN2) was characterized by cytological molecular studies on fibers transgenic green-colored cotton (Gossypium hirsutum) through three successive generations. Overexpression GhPFN2 caused pre-terminated cell elongation, resulting in...
Abstract Though used widely in cancer therapy, paclitaxel only elicits a response fraction of patients. A strong determinant tumor is the state microtubule dynamic instability. However, whether manipulation this physiological process can be controlled to enhance has not been tested. Here, we show previously unrecognized role microtubule-associated protein CRMP2 inducing bundling through its carboxy terminus. This activity significantly decreased when FER tyrosine kinase phosphorylates at...
Abstract Spinal cord injury (SCI) remains one of the most debilitating neurological disorders and majority SCI patients are in chronic phase. Previous studies have usually focused on few genes pathways at a time. In particular, biological roles long non-coding RNAs (lncRNAs) never been characterized SCI. Our study is first to comprehensively investigate alterations expression both coding sub-chronic stages using RNA-Sequencing. Through pathway analysis network construction, functions...
Contusive spinal cord injury (SCI) is the most common type of seen clinically. Several rat contusion SCI models have been described, and all strengths weaknesses with respect to sensitivity, reproducibility, clinical relevance. We developed Louisville Injury System Apparatus (LISA), which contains a novel spine-stabilizing device that enables precise stable spine fixation, based on tissue displacement determine severity injury. Injuries graded from mild moderately severe were produced using...
Abstract In the design and optimization of pneumatic feeding equipment for industrialized recirculating aquaculture systems (RAS), it is essential to simulate separating air-blowing process floating feed pellets through engineering discrete element model (EDEM). To improve accuracy (DEM), both computational simulations physical tests were performed adjust relevant values. A DEM was created by sampling measuring their inherent characteristics (density, Poisson ratio, shear modulus) along with...
Aumolertinib, a third-generation epidermal growth factor receptor tyrosine kinase inhibitor (EGFR-TKI), is widely utilized for advanced EGFR-mutant non-small cell lung cancer patients (NSCLCm). This single-arm, phase II trial (NCT04685070) assessed the feasibility of neoadjuvant Aumolertinib unresectable stage III NSCLCm. Fifty-six were enrolled, with 51 participants receiving (110 mg/day, orally) and forming intention-to-treat population. The primary endpoint was objective response rate...
As a potentially unlimited autologous cell source, patient induced pluripotent stem cells (iPSCs) provide great capability for tissue regeneration, particularly in spinal cord injury (SCI). However, despite significant progress made translation of iPSC-derived neural progenitor (NPCs) to clinical settings, few hurdles remain. Among them, non-invasive approach obtain source timely manner, safer integration-free delivery reprogramming factors, and purification NPCs before transplantation are...
The transplantation of neural stem/progenitor cells is a promising therapeutic strategy for spinal cord injury (SCI).In this study, we tested whether combination neurotrophic factors and glial-restricted precursor (GRPs)-derived astrocytes (GDAs) could decrease the promote functional recovery after SCI.We developed protocol to quickly produce sufficiently large, homogenous population young from GRPs, earliest arising progenitor cell restricted generation glia.GDAs expressed axonal...
Summary Rapid actin turnover is essential for numerous actin‐based processes. However, how it precisely regulated remains poorly understood. Actin‐interacting protein 1 ( AIP 1) has been shown to be an important factor by acting coordinately with actin‐depolymerizing ADF )/cofilin in promoting depolymerization, the rate‐limiting turnover. molecular mechanism which promotes largely unknown plants. Here, we provide a demonstration that turnover, required optimal growth of rice Specific...
Spinal cord injury (SCI) is one of the most devastating neural injuries without effective therapeutic solutions. Astrocytes are predominant component scar. Understanding complex contributions reactive astrocytes to SCI pathophysiologies fundamentally important for developing strategies. We have studied molecular changes in environment and astrocyte-specific responses by astrocyte purification from injured spinal cords acute chronic stages. In addition protein-coding genes, we systematically...