A5018307906- Spinal Cord Injury Research
- Nerve injury and regeneration
- Neurogenesis and neuroplasticity mechanisms
- Nerve Injury and Rehabilitation
- Traumatic Brain Injury and Neurovascular Disturbances
- Axon Guidance and Neuronal Signaling
- Spinal Dysraphism and Malformations
- Traumatic Brain Injury Research
- Advanced Algorithms and Applications
- Advanced Control Systems Design
- Pluripotent Stem Cells Research
- Fault Detection and Control Systems
- Mesenchymal stem cell research
- Stability and Control of Uncertain Systems
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Control Systems Optimization
- Photoreceptor and optogenetics research
- Neural Networks and Applications
- Mobile Ad Hoc Networks
- Neonatal Respiratory Health Research
- Face and Expression Recognition
- Iterative Learning Control Systems
- Liver Disease Diagnosis and Treatment
- MicroRNA in disease regulation
- Zebrafish Biomedical Research Applications
Wenzhou Medical University
2009-2025
Chongqing Medical University
2015-2025
The Affiliated Yongchuan Hospital of Chongqing Medical University
2025
Indiana University School of Medicine
2015-2024
Neurological Surgery
2015-2024
Indiana University – Purdue University Indianapolis
2015-2024
Affiliated Hospital of Jining Medical University
2024
Gansu Provincial Hospital
2024
Minhang District Central Hospital
2024
Zhejiang Chinese Medical University
2014-2024
Cell death was examined by studying the spinal cords of rats subjected to traumatic insults mild moderate severity. Within minutes after weight drop impact (a 10 gm falling 6.25 mm), neurons in immediate area showed a loss cytoplasmic Nissl substances. Over next 7 d, this lesion expanded and cavitated. Terminal deoxynucleotidyl transferase (TdT)-mediated deoxyuridine triphosphate–biotin nick end labeling (TUNEL)-positive were noted primarily restricted gross 4–24 hr injury, with maximum...
Abstract Schwann cells (SC) have been shown to promote regeneration in both the peripheral and central nervous systems. In this study we tested ability of SC enhance axonal adult rat spinal cord by grafting SC‐seeded guidance channels into transected cords. were purified culture from inbred sciatic nerves, suspended Matrigel, seeded semipermeable PAN/PVC (2.6 mm I.D. X 10 long) at a final density 120 × 6 cells/ml. Channels filled with Matrigel alone served as controls. Adult isologous cords...
We previously demonstrated that Schwann cells (SCs) in semipermeable guidance channels promote axonal regeneration adult rat spinal cord transected at the mid-thoracic level. Propriospinal but not supraspinal axons grew into these channels. Here, we tested ability of exogenous brain-derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) to this novel model. The two neurotrophins were delivered simultaneously channel by an Alzet minipump a rate 12 μg/day for each neurotrophin 14 30 days...
Axonal regeneration in the central nervous system (CNS) is a highly energy-demanding process. Extrinsic insults and intrinsic restrictions lead to an energy crisis injured axons, raising question of whether recovering deficits facilitates regeneration. Here, we reveal that enhancing axonal mitochondrial transport by deleting syntaphilin (Snph) recovers injury-induced depolarization. Using three CNS injury mouse models, demonstrate Snph−/− mice display enhanced corticospinal tract (CST)...
The pharmacological action of salicylate cannot be explained by its inhibition cyclooxygenase (COX) activity. In this report, the effects aspirin and sodium on COX-2 expressions in human umbilical vein endothelial cells foreskin fibroblasts were evaluated. Aspirin at therapeutic concentrations equipotently blocked mRNA protein levels induced interleukin-1β phorbol 12-myristate 13-acetate. suppressing effect was more pronounced cultured deprived fetal bovine serum for 24 h, suggesting that it...
Demyelination contributes to the physiological and behavioral deficits after contusive spinal cord injury (SCI). Therefore, remyelination may be an important strategy facilitate repair SCI. We show here that rat embryonic day 14 cord-derived glial-restricted precursor cells (GRPs), which differentiate into both oligodendrocytes astrocytes, formed normal-appearing central myelin around axons of cultured DRG neurons had enhanced proliferation survival in presence neurotrophin 3 (NT3)...
Abstract Schwann cells (SCs) have been shown to be a key element in promoting axonal regeneration after being grafted into the central nervous system (CNS). In present study, SC‐supported regrowth was tested an adult rat spinal cord implantation model. This model is characterized by right hemisection at eighth thoracic segment, of SC‐containing mini‐channel and restoration cerebrospinal fluid circulation suturing dura. We demonstrate that tissue cable containing SCs formed effective bridge...
To promote axonal regeneration in the injured adult spinal cord, a two-phase repair strategy was employed to (i) bridge cord hemilesion cavity with grafted Schwann cell (SC)-seeded mini-channel, and (ii) re-entry into distal by infusing two neurotrophins, BDNF and/or NT-3, directly parenchyma. Here we report that infusion of delivered alone or combination, effectively promotes outgrowth from SC-seeded mini-channels host cord. When an anterogradely transported marker, PHA-L BDA, injected 3 mm...
Demyelination contributes to the dysfunction after traumatic spinal cord injury (SCI). We explored whether combination of neurotrophic factors and transplantation adult rat oligodendrocyte precursor cells (OPCs) could enhance remyelination functional recovery SCI. Ciliary factor (CNTF) was most effective promote (OL) differentiation survival OPCs in vitro . were infected with retroviruses expressing enhanced green fluorescent protein (EGFP) or CNTF transplanted into contused thoracic 9 d...
Grafting of Schwann cell-seeded channels into hemisected adult rat thoracic spinal cords has been tested as a strategy to bridge the injured cord. Despite success in guiding axonal growth graft, regeneration across distal graft-host interface host cord was limited. We hypothesized that chondroitin sulfate (CS) glycoforms deposited at gliotic front constitute molecular barrier Because CS by purified astrocytes vitro were removable digestion with chondroitinase ABC, we attempted achieve...
Schwann cell (SC) grafts support the regeneration of axons numerous spinal cord neurons when placed into transected adult rat midthoracic cord. Clinically, methylprednisolone (MP) has been shown to be neuroprotective if administered within 8 h after injury. We investigated whether axonal regrowth SC is enhanced MP at time transection and implantation. SCs from sciatic nerves were purified in culture, suspended Matrigel, drawn semipermeable polymeric channels. (30 mg/kg) or vehicle (control)...
Failure of axon regeneration in the mammalian CNS is attributable part to presence various inhibitory molecules, including myelin-associated proteins and proteoglycans enriched glial scars. Here, we evaluate whether guidance molecules also regulate regenerative growth after injury adulthood. Wnts are a large family that can attract ascending axons repel descending along length developing spinal cord. Their expression (all 19 ) not detectable normal adult cord by situ hybridization. However,...
Abstract We previously demonstrated that coadministration of glial cell line‐derived neurotrophic factor (GDNF) with grafts Schwann cells (SCs) enhanced axonal regeneration and remyelination following spinal cord injury (SCI). However, the cellular target through which GDNF mediates such actions was unclear. Here, we report both number caliber regenerated axons in vivo increased neurite outgrowth dorsal root ganglion neurons (DRGN) vitro , suggesting has a direct effect on neurons. In...
Percolation theory is an approach to study the vulnerability of a system. We develop analytical framework and analyze percolation properties network composed interdependent networks (NetONet). Typically, single shows that damage in due failure continuous function size failure, i.e., fraction failed nodes. In sharp contrast, NetONet, cascading failures, transition may be discontinuous even node lead abrupt collapse demonstrate our general for NetONet n classic Erdős-Rényi (ER) networks, where...