A5101604914- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- Neurogenesis and neuroplasticity mechanisms
- MicroRNA in disease regulation
- Animal Genetics and Reproduction
- Spine and Intervertebral Disc Pathology
- Bone fractures and treatments
- Bone Metabolism and Diseases
- Orthopedic Surgery and Rehabilitation
- Circular RNAs in diseases
- RNA Research and Splicing
- Tissue Engineering and Regenerative Medicine
- Genetics and Neurodevelopmental Disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Congenital heart defects research
- Pelvic and Acetabular Injuries
- Cancer, Lipids, and Metabolism
- Ferroptosis and cancer prognosis
- Pancreatic function and diabetes
- Tendon Structure and Treatment
- Spinal Fractures and Fixation Techniques
- Cancer-related molecular mechanisms research
- Blood transfusion and management
- Bone and Joint Diseases
- Extracellular vesicles in disease
Affiliated Hospital of Shandong University of Traditional Chinese Medicine
2020-2025
General Hospital of Shenyang Military Region
2016-2024
Brown Foundation
2015-2024
Florida International University
2022-2024
Shandong University of Traditional Chinese Medicine
2020-2024
Yuncheng University
2024
Shanxi Medical University
2024
The University of Texas Health Science Center at Houston
2013-2020
Scripps Research Institute
2011-2020
University of California, San Diego
2011-2020
Glioblastoma (GBM) is one of the most deadly brain tumors. The convenient access to Cancer Genome Atlas (TCGA) database allows for large-scale global gene expression profiling and mining potential correlation between genes overall survival a variety malignancies including GBM. Previous reports have shown that tumor microenvironment cells extent infiltrating immune stromal in tumors contribute significantly prognosis. Immune scores calculated based on ESTIMATE algorithm could facilitate...
Down's syndrome (DS), caused by trisomy of human chromosome 21, is the most common genetic cause intellectual disability. Here we use induced pluripotent stem cells (iPSCs) derived from DS patients to identify a role for astrocytes in pathogenesis. astroglia exhibit higher levels reactive oxygen species and lower synaptogenic molecules. Astrocyte-conditioned medium collected causes toxicity neurons, fails promote neuronal ion channel maturation synapse formation. Transplantation studies show...
Abstract Lineally related multipotent neuroepithelial cells (NEP), neuronal restricted precursors (NRP), and glial (GRP) have been identified in the spinal cord. To determine sequence of differentiation identify lineage stage‐specific markers, we examined spatiotemporal expression established markers during rodent embryonic development within fetal cell culture. In this report, show that proliferating stem developing neural tube do not express any at E10.5. By E11, however, begun to...
It has previously been shown that the phage-derived phiC31 integrase can efficiently target native pseudo-attachment sites in genome of various species cultured cells, as well vivo. To demonstrate its utility human embryonic stem cells (hESC), we have created hESC-derived clones containing expression constructs. Variant cell lines BG01v and SA002 were used to derive expressing a green fluorescent protein (GFP) marker under control either Oct4 promoter or EF1alpha promoter. Stable selected by...
Abstract Background In order to compare the gene expression profiles of human embryonic stem cell (hESC) lines and their differentiated progeny monitor feeder contaminations, we have examined in seven hESC fibroblast cells using Illumina ® bead arrays that contain probes for 24,131 transcript probes. Results A total 48 different samples (including duplicates) grown multiple laboratories under conditions were analyzed pairwise comparisons performed all groups. Hierarchical clustering showed...
The mechanisms by which neural and glial progenitor cells in the adult brain respond to tissue injury are unknown. We studied responses of these stab wound rats two transgenic mouse models Y/GFP is driven either Sox2 (a stem cell marker) or Talpha-1 (which marks newly born neurons). response progenitors was low all nonneurogenic regions, no neurogenesis occurred at site. Glial expressing Olig2 NG2 showed greatest response. appearance preceded reactive astrocytes. Surprisingly, we found...
The focus of most neurodegenerative disease studies has been on neuronal death in particular subpopulations the central nervous system. associated response glial populations ascribed term "reactive astrocytosis." This defined as proliferation astrocytes accompanied by cellular hypertrophy and changes gene expression following injury to Yet significance that course is debated. In both human ALS SOD1G93A mouse model ALS, reactive astrocytosis a hallmark disease--particularly at endstage. brain...
Abstract In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, human embryonic stem cell (hESC) line BG01 by homologous recombination. One allele of Olig2 locus was replaced green fluorescent protein (GFP) cassette with targeting efficiency 5.7%. Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, normal parental karyotype 46,XY. Most importantly,...
It has been reported that metformin acts synergistically with temozolomide (TMZ) to inhibit proliferation of glioma cells including glioblastoma multiforme (GBM). However, the molecular mechanism underlying how exerts its anti-cancer effects remains elusive. We used a combined experimental and bioinformatics approach identify genes complex regulatory/signal transduction networks are involved in restoring TMZ sensitivity GBM after treatment. First, we established resistant cell lines found...
Abstract To access and compare gene expression in fetal neuroepithelial cells (NEPs) progenitor cells, we have used microarrays containing approximately 500 known genes related to cell cycle regulation, apoptosis, growth differentiation. We identified 152 that are expressed NEPs 209 by cells. The majority of (141) detected also present populations. There 68 specifically progenitors with little or no NEPs, a few appear be exclusively NEPs. Using sorting, RT–PCR, situ hybridization...
Abstract Glutamate is the major neurotransmitter of brain, whose extracellular levels are tightly controlled by glutamate transporters. Five transporters in human brain (EAAT1–5) present on both astroglia and neurons. We characterize profile three different astroglial progenitors vitro: glial restricted precursors (HGRP), astrocyte (HAPC), early‐differentiated astrocytes. EAAT 1, EAAT3, EAAT4 all expressed GRPs with a subsequent upregulation EAAT1 following differentiation into GRP‐derived...
The differentiation of human pluripotent stem cells (hPSCs) to insulin-expressing beta islet-like is a promising in vitro model system for studying the molecular signaling pathways underlying cell differentiation, as well potential source treatment type 1 diabetes. MicroRNAs (miRNAs) are class small non-coding RNAs that regulate many biological processes, including cellular differentiation. We studied miRNA and mRNA expression profiles hPSCs at five stages along pancreatic lineage...
As a potentially unlimited autologous cell source, patient induced pluripotent stem cells (iPSCs) provide great capability for tissue regeneration, particularly in spinal cord injury (SCI). However, despite significant progress made translation of iPSC-derived neural progenitor (NPCs) to clinical settings, few hurdles remain. Among them, non-invasive approach obtain source timely manner, safer integration-free delivery reprogramming factors, and purification NPCs before transplantation are...
Neural cell fate is determined by a tightly controlled transcription regulatory network during development. The ability to manipulate the expression of multiple factors simultaneously required delineate complex picture neural Because limited carrying capacity commonly used viral vectors, such as lentiviral or retroviral it often challenging perform perturbation experiments on factors. Here we have developed piggyBac (PB) transposon-based CRISPR activation (CRISPRa) all-in-one system, which...
Wiskott-Aldrich syndrome (WAS) is a severe X-linked primary immunodeficiency resulting from diversity of mutations distributed across all 12 exons the WAS gene. encodes hematopoietic-specific and developmentally regulated cytoplasmic protein (WASp). The objective this study was to develop gene correction strategy potentially applicable most patients by employing nuclease-mediated, site-specific integration corrective sequence into endogenous chromosomal locus. In study, we demonstrate...
SUMMARY While challenging, identifying individuals displaying resilience to Alzheimer’s disease (AD) and understanding the underlying mechanism holds great promise for development of new therapeutic interventions effectively treat AD. Down syndrome (DS), or trisomy 21, is most common genetic cause Interestingly, some people with DS, despite developing AD neuropathology, show cognitive decline. Furthermore, DS are at an increased risk myeloid leukemia due somatic mutations in hematopoietic...
Lineage reporters of human embryonic stem cell (hESC) lines are useful for differentiation studies and drug screening. Previously, we created reporter driven by an elongation factor 1 alpha (EF1α) promoter at a chromosome 13q32.3 locus in the hESC line WA09 abnormal BG01V site-specific manner. Expression these was maintained long-term culture undifferentiated state. However, when cells were differentiated into specific lineages, reduction expression observed, indicating transgene silencing....
Human iPS cells hold great promise for disease modeling and treatment of degenerative disorders including muscular dystrophies. Although a few research groups have used them skeletal muscle differentiation, most were based on gene over-expression or long-term mesenchymal differentiation retrospective identification myogenic cells. Therefore, this study was aimed to generate knock-in reporter human cell line MYF5, as an early specification gene, allow prospective purification progenitors from...
Directed differentiation of iPS cells toward various tissue progenitors has been the focus recent research. Therefore, generation tissue-specific reporter cell lines provides better understanding developmental stages in cells. This technical report describes an efficient strategy for and validation knock-in human using Cas9-nickase system. Here, we have generated a line early myogenic lineage specification gene PAX7. By introduction site-specific double-stranded breaks (DSB) genomic locus...