A5069486760- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Virus-based gene therapy research
- Selenium in Biological Systems
- Trace Elements in Health
- RNA Interference and Gene Delivery
- RNA modifications and cancer
- Animal Disease Management and Epidemiology
- Advanced biosensing and bioanalysis techniques
- RNA Research and Splicing
- Vector-Borne Animal Diseases
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- RNA and protein synthesis mechanisms
- Renal and related cancers
- HER2/EGFR in Cancer Research
- Cancer Treatment and Pharmacology
- RNA regulation and disease
- Heavy Metal Exposure and Toxicity
- Genetic Syndromes and Imprinting
- Monoclonal and Polyclonal Antibodies Research
- Developmental Biology and Gene Regulation
- Axon Guidance and Neuronal Signaling
China Agricultural University
2016-2025
Sanya University
2021-2025
Qinghai University
2023
Ministry of Agriculture and Rural Affairs
2023
Yantai University
2021-2022
Yangzhou University
2022
Chinese Academy of Sciences
2020
University of Minnesota
2014
University of Utah
2004-2014
Howard Hughes Medical Institute
1997-2009
Olig gene expression is proposed to mark the common progenitors of motoneurons and oligodendrocytes. In an attempt further dissect in vivo lineage relationships between oligodendrocytes, we used a conditional cell-ablation approach kill Olig-expressing cells. Although differentiated oligodendrocytes were eliminated, our ablation study revealed continuous generation subsequent death their precursors. Most remarkably, normal number oligodendrocyte precursors are formed at day 12 mouse...
Although CRISPR-Cas based genome editing has made significant strides over the past decade, achieving simultaneous homozygous gene of multiple targets in primary cells remains a challenge. In this study, we optimized coselection strategy to enhance rates genomes porcine fetal fibroblasts (PFFs). The utilizes expression surrogate reporter (eGFP) select for with highest expression, thereby improving efficiency. When applied multigene editing, targeted most challenging site selection, while...
Significance Because genome-wide CRISPR/Cas9 libraries are mostly constructed in lentiviral vectors, direct vivo screening has not been possible as a result of low efficiency delivery. Here, we examined the piggyBac (PB) transposon an alternative vehicle to deliver guide RNA (gRNA) library for screening. Through hydrodynamic tail vein injections, delivered PB-CRISPR into mouse liver. Rapid tumor formation could be observed less than 2 mo. By sequencing analysis PB-mediated gRNA insertions,...
To date no authentic embryonic stem cell (ESC) line or germline-competent-induced pluripotent (iPSC) has been established for large animals. Despite this fact, there is an impression in the field that animal ESCs iPSCs are as good mouse counterparts. Clarification of issue important a healthy advancement field. Elucidation causes failure obtaining high quality iPSCs/ESCs may offer essential clues eventual establishment animals including humans. end, we first generated porcine using...
The African swine fever virus (ASFV) has caused a devastating pandemic in domestic and wild swine, causing economic losses to the global industry. Recombinant live attenuated vaccines are an attractive option for ASFV treatment. However, safe effective against still scarce, more high-quality experimental vaccine strains need be developed. In this study, we revealed that deletion of genes DP148R, DP71L, DP96R from highly virulent isolate CN/GS/2018 (ASFV-GS) substantially virulence swine....
Abstract In this study, we targeted Olig2, a basic helix-loop-helix transcription factor that plays an important role in motoneuron and oligodendrocyte development, human embryonic stem cell (hESC) line BG01 by homologous recombination. One allele of Olig2 locus was replaced green fluorescent protein (GFP) cassette with targeting efficiency 5.7%. Targeted clone R-Olig2 (like the other clones) retained pluripotency, typical hESC morphology, normal parental karyotype 46,XY. Most importantly,...
Chicken egg lysozyme (cLY) is an enzyme with 129 amino acid (AA) residue enzyme. This present not only in chicken white but also mucosal secretions such as saliva and tears. The antibacterial properties of can be attributed to the presence lysozyme, which used anti-cancer drug for treatment human immunodeficiency virus (HIV) infection. In this study, we constructed a lentiviral vector containing synthetic cLY signal peptide 447 bp (hLY) cDNA sequence driven by oviduct-specific ovalbumin...
In Caenorhabditis elegans, homodimeric [kinesin family (KIF) 17, osmotic avoidance abnormal-3 (OSM-3)] and heterotrimeric (KIF3) kinesin-2 motors are required to establish sensory cilia by intraflagellar transport (IFT) where KIF3 KIF17 cooperate build the axoneme core builds distal segments. However, function of in vertebrates is unresolved. We expressed full-length motorless constructs mouse rod photoreceptors using adeno-associated virus Xenopus laevis a transgene ciliated IMCD3 cells....
Selenoprotein V (SELENOV) is a new and the least conserved member of selenoprotein family. Herein we generated Selenov knockout (KO) mice to determine its in vivo function. The KO led 16-19% increases (P < 0.05) body weight that were largely due 54% higher fat mass accumulation, compared with wild-type (WT) controls. extra accumulation was mediated by up-regulations genes proteins involved lipogenesis (Acc, Fas, Dgat, Lpl; up 40%-1.1-fold) down-regulations lipolysis (Atgl, Hsl, Ces1d, Cpt1a;...
Abstract During early embryonic development, particularly in the transition from totipotency to pluripotency, energy metabolism is closely linked cell fate. However, essential regulators of this remain unclear. In study, we reveal that Tcl1 influences metabolic characteristics and regulates totipotency–pluripotency transition. Our findings demonstrate absence triggers upregulation genes reduces H3K4me3 modifications at glycolysis enzyme promoters, thereby suppressing glycolytic processes....
ABSTRACT African swine fever virus (ASFV) is the pathogen of (ASF), and its infection causes a lethal disease in pigs, with severe pathological lesions. These changes indicate excessive inflammatory responses infected which main cause death, but ASFV proteins worked this physiological process mechanisms underlying ASFV-induced inflammation remain unclear. Here, we identify that viral I177L works these responses. Mechanistically, facilitates TRAF6 ubiquitination enhances binding to TAK1,...
Transformer models leverage self-attention mechanisms to capture complex dependencies, demonstrating exceptional performance in various applications. However, the long-duration high-load computations required for model inference impose stringent reliability demands on computing platform, as soft errors that occur during execution can significantly degrade performance. Existing fault tolerance methods protect each operation separately using decoupled kernels, incurring substantial...
The interaction between foot-and-mouth disease virus (FMDV) and the host is extremely important for infection, but there are few researches on it, which not conducive to vaccine development FMD control. In this study, we designed a porcine genome-scale CRISPR/Cas9 knockout library containing 93,859 single guide RNAs targeting 16,886 protein-coding genes, 25 long ncRNAs, 463 microRNAs. Using library, several previously unreported genes required FMDV infection highly enriched post-FMDV...